Method for improving high-temperature tolerance of pichia kudriavzevii

A yeast and high-temperature technology, applied in the field of biochemical industry, can solve the problems of high energy consumption and achieve the effects of convenient operation, simple method and reduced fermentation cost

Active Publication Date: 2016-07-13
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for improving the high temperature tolerance of Pichia kudori, which is beneficial to solve the problem of high energy consumption caused by the normal temperature fermentation of Pichia kudori and reduce the cost of fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Yeast activation: Inoculate the preserved Pichia kuderii on a fresh YEPD slant medium, and incubate at a constant temperature of 30°C for 24 hours. Inoculate the activated Pichia pichia prudori into YEPD liquid medium, and culture in a constant temperature shaker flask at 30° C. and 180 rpm for 24 hours.

[0021] (2) Yeast stress treatment: Inoculate the activated Pichia kurdori into YEPD liquid medium containing 6% sodium chloride for stress culture. The control culture conditions are: inoculum size 2%, constant temperature 30°C, The shaker rotates at 180rpm and cultivates for 24h.

[0022] (3) Yeast collection: Centrifuge the Pichia kurdori after the stress treatment at 4000rpm to collect the bacteria.

[0023] (4) Analysis of high temperature tolerance of yeast: Inoculate the obtained Pichia kurdori at a final concentration of 0.1 g / L (dry weight) into YEPD liquid medium, culture at a constant temperature of 45°C and a shaker speed of 180 rpm for 24 hours. The ...

Embodiment 2

[0025] (1) Yeast activation: inoculate the preserved Pichia kuderii on the fresh wort slant medium, and incubate at a constant temperature of 28°C for 30 hours. Inoculate the activated Pichia kurdori on the slant into the wort juice liquid medium, and culture it in a constant temperature shaker flask at 28° C. and 150 rpm for 36 hours.

[0026] (2) Yeast stress treatment: Inoculate the activated Pichia kurdori into the wort liquid medium containing 12% potassium chloride for stress culture. The control culture conditions are: inoculum size 6%, constant temperature 28°C , shaker speed 150rpm, cultivated for 48h.

[0027] (3) Collection of high-temperature-resistant yeast: Centrifuge the Pichia kurdori after the stress treatment at 3000 rpm to collect the bacteria.

[0028] (4) Analysis of high temperature tolerance of yeast: Inoculate the obtained Pichia kuderii into the wort liquid medium at a final concentration of 0.1g / L (dry weight), at a constant temperature of 45°C, at a...

Embodiment 3

[0030] (1) Yeast activation: inoculate the preserved Pichia kuderii on a fresh YEPD slant medium, and incubate at a constant temperature of 28°C for 18 hours. Inoculate the activated Pichia pedoriasis into the YEPD liquid medium, and culture it in a constant temperature shaker flask at 28° C. and 200 rpm for 18 hours.

[0031] (2) Yeast stress treatment: Inoculate the activated Pichia pichia into YEPD liquid medium containing 6% sodium nitrate for stress culture. The control culture conditions are: inoculum size 4%, constant temperature 28°C, shaking The bed rotation speed was 200 rpm, and cultured for 48 hours.

[0032] (3) Collection of high-temperature-resistant yeast: Centrifuge the Pichia kurdori after the stress treatment at 5000 rpm to collect the bacteria.

[0033] (4) Analysis of high temperature tolerance of yeast: Inoculate the obtained Pichia kuderii into YEPD liquid medium at a final concentration of 0.1 g / L (dry weight), keep the constant temperature at 45°C, sh...

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Abstract

The invention discloses a method for improving the high-temperature tolerance of pichia kudriavzevii.The method includes steps of (1), activating the pichia kudriavzevii, inoculating the activated pichia kudriavzevii in liquid cultivation media and cultivating the pichia kudriavzevii in the cultivation media; (2), carrying out stress treatment on the pichia kudriavzevii, to be more specific, inoculating the activated pichia kudriavzevii into liquid cultivation media with 0.1-20% of substances A and carrying out stress cultivation on the pichia kudriavzevii; (3), collecting high-temperature-tolerant pichia kudriavzevii, to be more specific, centrifugally collecting thalli from the pichia kudriavzevii after stress treatment on the pichia kudriavzevii is carried out and acquiring the high-temperature-tolerant pichia kudriavzevii.The substances A are salts.The method has the advantages that the high-temperature tolerance of the pichia kudriavzevii can be greatly improved, the method is favorable for solving the problem of high energy consumption due to normal-temperature fermentation of pichia kudriavzevii, the fermentation cost can be reduced, and important effects of promoting industrial application of the pichia kudriavzevii can be realized.

Description

technical field [0001] The invention relates to a method for improving the high-temperature tolerance of yeast, in particular to a method for improving the high-temperature tolerance of Pichia kurdori, belonging to the field of biochemical industry. Background technique [0002] The depletion of non-renewable resources such as oil and coal and the deterioration of the environment have become major obstacles to the sustainable development of human beings. The second-generation fuel ethanol has many advantages such as cleanness, wide range of raw material sources, and renewable. It is expected to replace traditional fossil energy and alleviate the increasingly serious energy crisis and environmental pollution faced by human society. Using microorganisms to convert sugarcane, corn, cassava and fiber waste into ethanol has become an ideal way to solve the world's energy crisis. [0003] Yeast is the most commonly used microorganism in ethanol fermentation production. The optimu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/16C12R1/84
CPCC12N1/16C12N1/38
Inventor 李春生杨贤庆李来好杨少玲赵永强马海霞吴燕燕戚勃胡晓邓建朝
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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