Primer and probe as well as kit used for avian influenza virus universal type direct RT-PCR detection

Abstract

The invention discloses a primer and a probe as well as a kit used for avian influenza virus universal type direct RT-PCR detection. The primer and the probe comprise a detecting primer and a detecting probe of the avian influenza virus as well as a probe of internal label RNA. With the adoption of the primer and the probe, a liquid sample can be directly added into a reaction system, so that release, reverse transcription and fluorescent quantitative PCR detection of the virus RNA are continuously and automatically realized in one PCR tube; the purposes of extracting the virus RNA and directly detecting the avian influenza virus in the extracted sample are realized, so that only 1.5 hours are needed for obtaining detected results from a to-be-detected sample; and the defect that RNA is needed to be extracted by conventional RT-PCR is effectively overcome, operation steps can be greatly reduced, the detection speed is greatly increased, the cost is reduced, and cross contamination is effectively avoided.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a primer, a probe and a kit for general direct RT-PCR detection of avian influenza virus. Background technique [0002] Avian influenza (Avian Influenza, AI), also known as European chicken plague or true chicken plague, is an acute and highly contagious infectious disease caused by type A influenza virus. It is an internationally recognized devastating disease and can be transmitted to humans by pigs or other animals. Therefore, it has very important ecological and public health significance, especially highly pathogenic avian influenza is listed as a class A infectious disease by the World Organization for Animal Health. [0003] In recent years, it has been found that avian influenza not only seriously harms poultry but also mammals, especially can infect and cause death of humans, which greatly threatens the safety of human public health. The avian influenza virus is...

AI Technical Summary

Problems solved by technology

Adopt primer and probe or test kit of the present invention, and in conjunction with other conventional reagent components, can realize in a PCR tube continuously, automatically carry out the release of virus RNA, reverse transcription and fluorescent quantitative PCR detection; From obtaining detection sample to It only takes 1.5 hours to obtain the test results; the sensitivity and accuracy are comparable to traditional RT-PCR; it overcomes the shortcomings of traditional RT-PCR that needs to extract RNA, which is a cumbersome step, reduces operating steps, improves detection speed, and saves costs. Effectively avoid cross-contamination and high-throughput detection of avian influenza virus, which is of great significance for the rapid detection of avian influenza and the timely formulation of prevention and control measures

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