Preparation of carboxymethyl cellulose-chitosan composite material and identification of tryptophan enantiomer by modified electrode of composite material through electrochemical process

A technology of carboxymethyl cellulose and tryptophan enantiomers, which is applied in the field of biotechnology and electrochemical research, can solve the problems of difficult online detection, high cost, and poor reproducibility of capillary electrophoresis, and achieves a simple preparation method The effect of environmental protection and good recognition ability

Active Publication Date: 2016-07-13
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, chromatography has been widely used in the separation and analysis of chiral compounds, and it has been proved to be an effective chiral analysis method, but the method is costly and takes a long time to analyze, and it is difficult to realize online detection
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Method used

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  • Preparation of carboxymethyl cellulose-chitosan composite material and identification of tryptophan enantiomer by modified electrode of composite material through electrochemical process
  • Preparation of carboxymethyl cellulose-chitosan composite material and identification of tryptophan enantiomer by modified electrode of composite material through electrochemical process
  • Preparation of carboxymethyl cellulose-chitosan composite material and identification of tryptophan enantiomer by modified electrode of composite material through electrochemical process

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Experimental program
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Effect test

Embodiment 1

[0029] Take sodium carboxymethyl cellulose powder and dissolve it in 50mL of 0.1M sodium dihydrogen phosphate (pH=7) solution, stir to dissolve it completely, configure it into a 2mg / mL sodium carboxymethyl cellulose solution, and then add it to the solution Add 50 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and 50 mg of N-hydroxysuccinimide, and stir for 2 h to activate the carboxyl groups in sodium carboxymethylcellulose. Get chitosan powder and dissolve in 50mL0.1M acetic acid solution, stir to make it dissolve completely, and configure 2mg / mL chitosan solution. The chitosan solution is added dropwise in the sodium carboxymethyl cellulose solution, and the stirring is continued during the dropping process. The precipitated white solid is a carboxymethyl cellulose-chitosan composite material, which is centrifuged, and the obtained composite material is Rinse 3 times with 0.1M acetic acid solution to remove unreacted chitosan, then wash the composite mate...

Embodiment 2

[0031] Preparation of carboxymethyl cellulose-chitosan composite material and its modified electrode The electrochemical method for identifying tryptophan enantiomers includes the following steps:

[0032] (1) Ultrasonic disperse the carboxymethyl cellulose-chitosan composite material in ultrapure water, pipette 5 μL (2 mg / mL) of the dispersion liquid onto the surface of the glassy carbon electrode, and dry at room temperature .

[0033] (2) After the prepared carboxymethylcellulose-chitosan composite modified electrode was placed in 25mL / D-tryptophan solution for 60s, the electrochemical window range of 0.4-1.2V (vs.SCE) Differential pulses were performed within each measurement, and the modified electrode was swept in 25mL of 0.1M sodium dihydrogen phosphate (pH=7) to restore the activity of the electrode after each measurement. The recognition effect of tryptophan enantiomers by carboxymethylcellulose-chitosan composite modified electrode is shown in the appendix figure ...

Embodiment 3

[0035] In order to study the differences in the ability of carboxymethylcellulose-chitosan composite modified electrodes to recognize tryptophan enantiomers at different temperatures. Therefore, the differences in the recognition efficiency of tryptophan enantiomers by the composite were examined at 6°C, 8°C, 10°C, 15°C, 20°C, 25°C, 30°C and 35°C, respectively. The effects of recognition of tryptophan enantiomers by carboxymethyl cellulose-chitosan composite modified electrodes at different temperatures are shown in the appendix image 3 , it can be seen that when the temperature is 25 ℃, the recognition effect of the carboxymethyl cellulose-chitosan composite modified electrode on the tryptophan enantiomer reaches the best.

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Abstract

The invention relates to preparation of a carboxymethyl cellulose-chitosan composite material and identification of a tryptophan enantiomer by a modified electrode of the composite material through an electrochemical process. The preparation comprises the following steps of: preparing the carboxymethyl cellulose-chitosan composite material; preparing the electrode modified by the carboxymethyl cellulose-chitosan composite material; and identifying the tryptophan enantiomer through the electrochemical process. The preparation and the identification, provided by the invention, have the beneficial effects that the preparation method of the carboxymethyl cellulose-chitosan composite material is simple and environmentally friendly; the electrode modified by the carboxymethyl cellulose-chitosan composite material has a relatively good identification capability on the tryptophan enantiomer. Carboxymethyl cellulose and chitosan have a selectively synergistic effect on the tryptophan enantiomer.

Description

technical field [0001] The invention relates to the preparation of a carboxymethyl cellulose-chitosan composite material and its modified electrode electrochemical method for identifying tryptophan enantiomers, belonging to the fields of biotechnology and electrochemical research. technical background [0002] Substances that constitute life activities, such as proteins, polysaccharides, nucleic acids, amino acids, etc., are almost all chiral. For chiral compounds, when there is no external chiral environment, the two enantiomers have exactly the same chemical and physical properties except for optical activity, as well as have the same infrared and nuclear magnetic resonance spectra, in gas and liquid chromatography also have the same retention time. However, when an external chiral environment exists, the two optical isomers exhibit different properties (such as pharmacodynamics and pharmacokinetics). Therefore, chiral recognition has important research significance. Cu...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 孔泳鲍丽平陶永新
Owner CHANGZHOU UNIV
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