Unlock instant, AI-driven research and patent intelligence for your innovation.

Tissue culture method of hemerocallis middendorfii 'lace scarf'

A large-flowered Hemerocallis, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low seed setting rate of Hemerocallis, limitation of large-scale promotion of new varieties of Hemerocallis, low quantity and high price, and achieves easy growth. bud effect

Active Publication Date: 2016-07-20
宿迁市农业科学研究院
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seed setting rate of Hemerocallis is low, and the growth performance of seed seedlings varies greatly, and the variety stability is low. Generally, seeds are not used for propagation, but tillering is used for asexual reproduction.
However, due to its slow speed, generally one Hemerocallis grandiflorum can only reproduce 3 to 4 plants per year, and individual varieties can only reproduce 1 to 2 plants per year, so it is difficult to meet the needs of commercial production in the market, resulting in the current high-quality Hemerocallis varieties with low quantity and high price. current situation, which seriously limits the large-scale promotion of new daylilies in China

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The tissue culture method of Hemerocallis grandiflora "lace square scarf", the method comprises the following steps:

[0029] (1) The acquisition of sterile materials, the stem tip of Hemerocallis grandiflora "Lace Square" can be taken all year round, cleaned and cut into 3 small pieces of 1 cm, soaked in 4-5% Shannong No. 1 type I solution for 16 hours , add 1 / 4 salts of MS basic medium to the soaking solution, the MS basic medium adopts white sugar 20g / L, agar 5g / L, pH5.8, take it out and inoculate it on the callus induction medium, the callus induction medium is MS+ Shannong No. 1 Type II 0.1%+6—BA1.8mg / L+NAA0.3mg / L;

[0030] (2) Callus induction: under the conditions of 25±2°C, light intensity 1500Lx, and light 12h / d, obvious callus tissue can be seen at the shoot tip after 40 days, and cultured in dark for 20 days;

[0031] (3) Bud differentiation: connect the callus to the bud differentiation induction medium, the bud differentiation induction medium is MS+Shanno...

Embodiment 2

[0038] The tissue culture method of Hemerocallis grandiflora "lace square scarf", the method comprises the following steps:

[0039] (1) The acquisition of sterile materials, the stem tip of Hemerocallis grandiflora "Lace Square" can be taken all year round, cleaned and cut into 3 small pieces of 1 cm, soaked in 4-5% Shannong No. 1 type I solution for 16 hours , add 1 / 4 salts of MS basic medium to the soaking solution, the MS basic medium adopts white sugar 20g / L, agar 5g / L, pH5.8, take it out and inoculate it on the callus induction medium, the callus induction medium is MS+Shannong No. 1 Type II 0.1%+6—BA2.0mg / L+NAA0.5mg / L;

[0040] (2) Callus induction: under the conditions of 25±2°C, light intensity 2000Lx, and light 12h / d, obvious callus tissue can be seen at the shoot tip after 40 days, and cultured in dark for 25 days;

[0041] (3) Bud differentiation: connect the callus to the bud differentiation induction medium, the bud differentiation induction medium is MS+Shannon...

Embodiment 3

[0048] The tissue culture method of Hemerocallis grandiflora "lace square scarf", the method comprises the following steps:

[0049] (1) The acquisition of sterile materials, the stem tip of Hemerocallis grandiflora "Lace Square" can be taken all year round, cleaned and cut into 3 small pieces of 1 cm, soaked in 4-5% Shannong No. 1 type I solution for 16 hours , add 1 / 4 salts of MS basic medium to the soaking solution, the MS basic medium adopts white sugar 20g / L, agar 5g / L, pH5.8, take it out and inoculate it on the callus induction medium, the callus induction medium is MS+Shannong No. 1 Type II 0.1%+6—BA2.3mg / L+NAA0.6mg / L;

[0050] (2) Callus induction: under the conditions of 25±2°C, light intensity 2500Lx, and light 12h / d, obvious callus tissue can be seen at the shoot tip after 40 days, and cultured in dark for 30 days;

[0051] (3) Bud differentiation: connect the callus to the bud differentiation induction medium, the bud differentiation induction medium is MS+Shannon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture method of hemerocallis middendorfii 'lace scarf'. The method comprises the following steps: (1) obtaining sterile materials; (2) inducing calluses; (3) differentiating buds: (4) carrying out rejuvenation cultivation; (5) carrying out rooting culture; (6) hardening seedlings and transplanting. According to the invention, shoot tips of the hemerocallis middendorfii 'lace scarf' are served as explants, and the explants are used for disinfection and optimization of callus induction, bud differentiation, rejuvenation, a rooting medium formula and culture conditions of seedling hardening, so as to realize rapid propagation of the seedlings of the hemerocallis middendorfii 'lace scarf' through stages of callus induction, bud differentiation, rejuvenation, rooting culture and test-tube plantlet transplanting, the survival rate of tissue culture seedlings is 98% or above, and a foundation is laid for the rapid propagation and popularization and application of the hemerocallis middendorfii.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture of Hemerocallis grandiflora "lace scarf". Background technique [0002] Hemerocallis grandiflora is a perennial herb of Liliaceae Hemerocallis genus. The flowers of Hemerocallis grandiflora bloom in summer with few flowers. There are yellow, apricot yellow, orange, carmine and purple and other flower colors. It has a long flowering period and a large amount of flowers. Strong drought resistance, cold resistance, salinity resistance, disease and insect pest resistance, semi-shade resistance and other characteristics. Hemerocallis is an important garden plant in European and American countries, and it is one of the most abundant perennial flowers. The research and utilization of Hemerocallis in my country started relatively late, and currently only one species of Hemerocallis has been successfully promoted and applied. Most of the varieties of Hemerocallis ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 陈芬金倩罗桂杰蔡卫佳王昊刘博刘旭
Owner 宿迁市农业科学研究院