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Quick identifying method for positive and negative food-borne pathogenic bacteria

A food-borne pathogenic bacteria-negative technology, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, to achieve the effects of simple preparation, fast detection speed, and easy operation

Inactive Publication Date: 2016-07-20
JIANGSU UNIV
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Problems solved by technology

[0003] At present, there is no report on the classification and identification of positive and negative foodborne pathogenic bacteria using graphite carbonized nitrogen / gold or graphite carbonized nitrogen / silver nanoparticle composites as an enhancing reagent combined with surface-enhanced Raman scattering technology and chemometric methods

Method used

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  • Quick identifying method for positive and negative food-borne pathogenic bacteria
  • Quick identifying method for positive and negative food-borne pathogenic bacteria

Examples

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Embodiment 1

[0024] In order to further verify the classification and identification effect of the method of the present invention on positive and negative foodborne pathogenic bacteria, examples of the present invention, with three kinds of positive pathogenic bacteria (staphylococcus aureus, mycobacterium smegmatis, listeria) Taking three negative pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium) as an example, the specific operation steps are as follows:

[0025] (1) Prepare slant culture medium of 6 kinds of bacteria, inoculate it, cultivate it at 37°C for 8-12 hours, and then fix it as a slant; inoculate 6 kinds of bacteria on the solid slant into 6 liquid culture medium, shake it under the condition of 37°C Rotate at 150-200r / min, cultivate for 12-16h.

[0026] (2) Gradiently dilute the bacterial solutions of 6 positive and negative food-borne pathogenic bacteria, select the appropriate gradient to spread on the plate, and cultivate in a 37°C incu...

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Abstract

The invention discloses a quick identifying method for positive and negative food-borne pathogenic bacteria.The method comprises the steps that a graphite carbonization nitrogen / gold or graphite carbonization nitrogen / silver nanoparticle composite is adopted as enhancing reagent, special structure differences of cell walls of the positive and negative food-borne pathogenic bacteria are used, and by means of a surface enhanced raman spectrum and a chemometrics method, successful quick identification is conducted on the positive and negative food-borne pathogenic bacteria.According to the method, sensitivity is high, reliability is strong, the detecting speed is high, and cost is low; the method can be used for large-scale on-line detection of the positive and negative food-borne pathogenic bacteria and is suitable for the technical fields of food safety, environment monitoring and the like.

Description

technical field [0001] The invention relates to a rapid identification method for positive and negative food-borne pathogenic bacteria, in particular to a rapid classification and identification method for positive and negative food-borne pathogenic bacteria in combination with surface-enhanced Raman spectroscopy and a chemometric method. Background technique [0002] In recent years, microbial contamination has penetrated into water and food, and diseases caused by specific microorganisms are increasing, so rapid microbial detection methods have attracted widespread attention. According to literature search, the commonly used microbial detection methods mainly include traditional microbial detection technology, molecular biology detection technology, instrumental analysis technology and immunological detection technology. Although these existing detection methods have their own advantages, they all have certain limitations, such as complex pre-processing steps, long time, h...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李欢欢陈全胜欧阳琴郭志明林颢胡薇薇张彬王名星
Owner JIANGSU UNIV
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