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Analytical test method for ractopamine

A technology for analysis and testing of ractopamine, applied in the direction of analyzing materials, measuring devices, material separation, etc., can solve the problem of low adsorption capacity

Active Publication Date: 2019-02-01
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods are easy to operate and can selectively extract β 2 - Adrenergic receptor agonist, but the adsorption capacity is not high

Method used

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  • Analytical test method for ractopamine
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  • Analytical test method for ractopamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] For the analytical test method of ractopamine, the steps are as follows:

[0045] A, aluminum oxide film is carried out ATRP initiator surface modification treatment;

[0046] B. Synthesize ractopamine by atom transfer radical polymerization on aluminum oxide film to prepare AAO-MIP nanotube film;

[0047] C, prepare pork sample;

[0048] D. Add 1.5 μg / g of ractopamine and 15 μg / g of clenbuterol to the pork sample;

[0049] E, add 10mL acetonitrile saturated n-hexane solution in pork sample;

[0050] F. Add 1 mL of 4mol / L potassium carbonate solution to the pork sample to denature the protein, and take out the fatty acid and water;

[0051] G, acetonitrile solution, potassium carbonate solution are mixed with pork sample, remove the fat in pork sample, make mixture;

[0052] H, the mixture was shaken vigorously, the shaking time was 5 minutes, and the acetonitrile layer was separated out;

[0053] 1. Add 20mL saturated NaCl solution to prevent emulsification of the...

Embodiment 2

[0060] For the analytical test method of ractopamine, the steps are as follows:

[0061] A, aluminum oxide film is carried out ATRP initiator surface modification treatment;

[0062]B. Synthesize ractopamine by atom transfer radical polymerization on aluminum oxide film to prepare AAO-MIP nanotube film;

[0063] C, prepare pork sample;

[0064] D, add the ractopamine of 1.8 μ g / g in the pork sample, add the terbutaline of 18 μ g / g;

[0065] E, add 15mL acetonitrile saturated n-hexane solution in pork sample;

[0066] F, add 2mL 4mol / L potassium carbonate solution in pork sample, make protein denaturation, take out fatty acid and water;

[0067] G, acetonitrile solution, potassium carbonate solution are mixed with pork sample, remove the fat in pork sample, make mixture;

[0068] H, the mixture was shaken vigorously, the shaking time was 8 minutes, and the acetonitrile layer was separated out;

[0069] 1. Add 22mL saturated NaCl solution to prevent emulsification of the mi...

Embodiment 3

[0076] For the analytical test method of ractopamine, the steps are as follows:

[0077] A, aluminum oxide film is carried out ATRP initiator surface modification treatment;

[0078] B. Synthesize ractopamine by atom transfer radical polymerization on aluminum oxide film to prepare AAO-MIP nanotube film;

[0079] C, prepare pork sample;

[0080] D, add the ractopamine of 2.0 μ g / g in pork sample, add the epinephrine of 20 μ g / g;

[0081] E, add 18mL acetonitrile saturated n-hexane solution in pork sample;

[0082] F, add 3mL 4mol / L potassium carbonate solution in pork sample, make protein denaturation, take out fatty acid and water;

[0083] G, acetonitrile solution, potassium carbonate solution are mixed with pork sample, remove the fat in pork sample, make mixture;

[0084] H, the mixture was shaken vigorously, the shaking time was 10 minutes, and the acetonitrile layer was separated out;

[0085] 1. Add 24mL saturated NaCl solution to prevent emulsification of the mixt...

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Abstract

The invention provides an analysis test method for ractopamine, an AAO-MIP nanotube membrane extraction and high performance liquid chromatography combined detection method is established, and a beta 2-adrenocepter agonist in complex samples is detected.

Description

【Technical field】 [0001] The invention relates to an analysis and test method for ractopamine. 【Background technique】 [0002] Ractopamine (RAC) belongs to β 2 -Adrenergic receptor agonist drugs can promote animal muscle growth and protein accumulation, but excessive use will lead to its accumulation in animal tissues, which may have a negative impact on human health. Therefore, it is particularly important to detect the residual amount of ractopamine (RAC) in the sample. [0003] The detection methods currently used for ractopamine include gas chromatography, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, molecularly imprinted polymer extraction-liquid chromatography and molecular Imprinted polymer electrochemical sensors, etc. Regardless of the analytical method used, steps of enrichment and elution are necessary to reduce detection limits and eliminate interferences. [0004] Molecular impr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈忻梁勇陈晓刚赵亮亮沈国权潘嘉慧
Owner FOSHAN UNIVERSITY
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