Culture method of vigorous test-tube seedlings of natural Vaccinium uliginosum in Greater Khingan range

A technology of bilberry and cultivation method, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult rooting and transplanting, slender test-tube seedlings, low survival rate, etc., to improve the effective utilization rate , the effect of reducing production waste and reducing production costs

Inactive Publication Date: 2016-07-27
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems that the test-tube seedlings of the existing Daxinganling natural lingonberry tissue culture are slender, weak in grow

Method used

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Experimental program
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specific Embodiment approach 1

[0012] Specific implementation mode one: the cultivation method of the natural Dusi lingonberry test tube strong seedlings in Daxing'an Mountains in this embodiment is specifically carried out according to the following steps:

[0013] 1. Induction culture: Using the axillary buds of the natural Vaccinium vaccinium in Daxing’an Mountains as explants, the axillary buds of Vaccinium vaccinium were sterilized by conventional tissue culture and then transferred to the induction medium in the ultra-clean workbench for induction culture for 18 days~ 22d, obtain the axillary bud after induction culture;

[0014] 2. Differentiation culture: transfer the axillary buds after the induction culture obtained in step 1 to the differentiation medium and carry out differentiation culture for 50d to 70d to obtain thin and weak seedlings of Vaccinium vaccinium; the average height of the vaccinium vaccinium seedlings is 6cm;

[0015] 3. Cultivation of strong seedlings: pour the strong seedling m...

specific Embodiment approach 2

[0017] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the conventional tissue culture disinfection described in step one is to wash the axillary buds of Vaccinium cranberry with running water for 10 minutes, and then add 0.1% HgCl in the ultra-clean workbench 2 Fully soak and disinfect for 2.5 minutes, then rinse with sterile water for 3 times, soak in sterile water for 40 minutes, take out, and use sterile filter paper to dry the surface moisture of the material. Others are the same as the first embodiment.

specific Embodiment approach 3

[0018] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the induction medium described in step one is: DY basic medium + zeatin 0.5 mg / L + sucrose 25 g / L ~ 35 g / L + strength of 1400g / cm 2 Agar 6.0g / L~7.0g / L, pH value 5.5~6.0;

[0019] KNO in the DY basic medium 3 The content is 180mg / L~200mg / L, NH 4 NO 3 The content is 400mg / L~410mg / L, MgSO 4 ·7H 2 O content is 365mg / L~380mg / L, KH 2 PO 4 The content is 160mg / L~180mg / L, Ca(NO 3 ) 2 4H 2 O content is 680mg / L~690mg / L, H 3 BO 3 The content is 6.3mg / L, MnSO 4 4H 2 O content is 22.4mg / L, ZnSO 4 ·7H 2 O content is 8.5mg / L, Na 2 MoO 4 2H 2 O content is 0.24mg / L, CuSO 4 ·5H 2 O content is 0.24mg / L, C 10 h 13 FeN 2 NaO 8 The content is 73mg / L~74mg / L, Na 2 The content of EDTA is 37mg / L~38mg / L, the content of thiamine hydrochloride is 0.05mg / L~0.15mg / L, the content of niacin is 0.45mg / L~0.55mg / L, the content of pyridoxine hydrochloride 0.5mg / L~0.6mg / L, the c...

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Abstract

The invention relates to a culture method of vigorous test-tube seedlings of natural Vaccinium uliginosum in the Greater Khingan range and aims to solve the problems that tissue cultured test-tube seedlings of the natural Vaccinium uliginosum in the Greater Khingan range are slender, the growth vigor is poor, rooting and transplanting are difficult and the survival rate is low. The culture method comprises steps as follows: step one, inducing culture; step two, differentiation culture; step three, vigorous seedling culture. With the adoption of the culture media and the culture method, the diameter can be increased by about 68.7% through 60-day rejuvenation culture, the transplanting survival rate of the tissue cultured seedlings is greatly increased, the production waste is reduced, and the production cost is reduced. The culture method is applied to vigorous seedling culture of the natural Vaccinium uliginosum in the Greater Khingan range.

Description

technical field [0001] The invention relates to a method for cultivating natural lingonberry test-tube seedlings in Daxing'an Mountains. Background technique [0002] Dustin lingonberry fruit is a delicious fruit rich in vitamins and minerals. At the same time, it has special effects such as removing free radicals in the body, delaying aging, anti-cancer, and enhancing human immunity. It is known as the "king of the third-generation fruit in the world". The natural Dusi lingonberries in the Greater Khingan Mountains have the characteristics of blue skin and blue flesh, higher anthocyanin content, and are more suitable for deep processing of products; and the local varieties are fully adapted to the local cold climate characteristics, and can survive the winter safely without additional heat preservation. It is very promising to reduce the loss of manpower and material resources. However, due to people's predatory picking, the natural huckleberry resources in the Daxing'anl...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 田新华李京张建瑛翁海龙卢慧颖李艳霞邢亚娟
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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