Tobacco additive prepared from fresh tobacco leaves as well as preparation method and application of tobacco additive
A technology of fresh tobacco leaves and additives, applied in the field of cigarette flavors and fragrances, can solve the problems of long purification cycle, occupying warehouse space and human resources, and inability to alcoholize tobacco leaves, and achieves the improvement of tobacco quality, wide range of raw material sources, and improved aroma and taste. Effect
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Embodiment 1
[0052] A preparation method of a tobacco additive derived from fresh tobacco leaves, comprising the following steps:
[0053] Picking the mature middle tobacco leaves, washing the surface of the tobacco leaves, removing the main veins of the tobacco leaves, and then mixing the tobacco leaves with the main veins removed with a phosphate buffer solution at a temperature of 6°C in a mass-volume ratio of 1:8 to obtain a mixture; the phosphoric acid The buffer solution has a pH value of 7 and a concentration of 0.03M;
[0054] Then the mixture is crushed by a crusher at 6°C until the diameter of the crushed residue is less than 2mm;
[0055] The crushed mixture was filtered through 5 layers of sterile gauze and the residue was discarded, and the filtrate was concentrated by filtration through a 0.22um hollow fiber column to 18% of the original volume;
[0056] Then the concentrate is naturally settled at 3°C for more than 10 hours until it is divided into 3 layers, and the middl...
Embodiment 2
[0060] A preparation method of a tobacco additive derived from fresh tobacco leaves, comprising the following steps:
[0061] Pick the mature middle tobacco leaves, wash the surface of the tobacco leaves and remove the main veins of the tobacco leaves, then mix the tobacco leaves with the main veins removed with a phosphate buffer solution at a temperature of 10°C in a mass-volume ratio of 1:8 to obtain a mixture; the phosphoric acid The buffer solution has a pH value of 7 and a concentration of 0.05M;
[0062] Then the mixture is crushed by a crusher at 10°C until the diameter of the crushed residue is less than 2mm;
[0063] The crushed mixture was filtered through 5 layers of sterile gauze and the residue was discarded, and the filtrate was concentrated by filtration through a 0.22um hollow fiber column to 22% of the original volume;
[0064] Then the concentrate is naturally settled at 8°C for more than 8 hours until it is divided into 3 layers. The middle layer material ...
Embodiment 3
[0068] A preparation method of a tobacco additive derived from fresh tobacco leaves, comprising the following steps:
[0069] Picking the mature middle tobacco leaves, washing the surface of the tobacco leaves, removing the main veins of the tobacco leaves, and then mixing the tobacco leaves with the main veins removed with a phosphate buffer solution at a temperature of 4°C in a mass-volume ratio of 1:12 to obtain a mixture; the phosphoric acid The buffer solution has a pH value of 7 and a concentration of 0.01M;
[0070] Then the mixture is crushed by a crusher at 4°C until the diameter of the crushed residue is less than 2mm;
[0071] The crushed mixture was filtered through sterile gauze and the residue was discarded, and the filtrate was concentrated by filtration through a 0.22um hollow fiber column to 20% of the original volume;
[0072] Then the concentrate was naturally settled at 4°C for more than 5 hours, and then divided into three layers, and the middle layer mat...
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