Method for preparing molecularly imprinted monolithic column

A technology of molecular imprinting and monolithic column, which is applied in the field of molecular imprinting chromatography, can solve problems such as insoluble, expensive, and interference in the determination of trace analytes, and achieve good imprinting effect and high recognition effect

Inactive Publication Date: 2016-07-27
GUANGDONG ENG TECH INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the molecularly imprinted polymer monolithic column prepared by the template molecule method showed high selectivity, but due to the thicker polymer matrix, the recognition sites and residual template molecules were deeply buried in the polymer matrix, which was difficult to achieve. Very serious template leakage problem. The residual template molecules in the polymer leak during solid-phase extraction, which will greatly interfere with the determination of trace analytes; Difficult to dissolve in solution system

Method used

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  • Method for preparing molecularly imprinted monolithic column

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Embodiment 1

[0029] A method for preparing a molecularly imprinted monolithic column, the preparation steps are as follows:

[0030] A, choose 1.5mmol methacrylic acid as functional monomer, 0.125mmol sulfamethoxazole (SMO) as false template molecule, 13.5mmol ethylene glycol dimethacrylate as cross-linking agent, false template molecule, functional monomer Add the cross-linking agent to the porogen solution of dimethyl sulfoxide (DMSO)-n-dodecyl alcohol system / water, mix and stir, and ultrasonically induce solubilization for 5 minutes to form a mixed solution of imprinted molecules and functional monomers;

[0031] B, the above-mentioned mixed solution-made is deoxygenated by nitrogen, and the nitrogen time is 10 minutes;

[0032] C, add 23.8mg initiator azobisisobutyronitrile in mixed solution one after logical nitrogen deoxygenation, make mixed solution two;

[0033] D. Pour the mixed solution II into a 100mm×4.6mm stainless steel chromatographic column tube I and seal it, and react in...

Embodiment 2

[0038] A method for preparing a molecularly imprinted monolithic column, the preparation steps are as follows:

[0039] A, choose 1.32mmol methacrylic acid as functional monomer, 0.12mmol sulfamethoxazole (SMO) as pseudo-template molecule, 12.0mmol ethylene glycol dimethacrylate as cross-linking agent, the pseudo-template molecule, functional monomer Add the cross-linking agent to the porogen solution of dimethyl sulfoxide (DMSO)-n-dodecyl alcohol system / water, mix and stir, and ultrasonically induce solubilization for 5 minutes to form a mixed solution 1 of imprinted molecules and functional monomers;

[0040] B, the above-mentioned mixed solution-made is deoxygenated by nitrogen, and the nitrogen time is 10 minutes;

[0041] C, add 23.8mg initiator azobisisobutyronitrile in the mixed solution one after nitrogen deoxygenation, make the mixed solution two;

[0042] D. Pour the mixed solution 2 into the 100mm×4.6mm stainless steel chromatographic column tube 1 and seal it insi...

Embodiment 3

[0049] A method for preparing a molecularly imprinted monolithic column, the preparation steps are as follows:

[0050] A, choose 1.3mmol methacrylic acid as functional monomer, 0.13mmol sulfamethoxazole (SMO) as pseudo-template molecule, 13.65mmol ethylene glycol dimethacrylate as cross-linking agent, make pseudo-template molecule, functional monomer Add the cross-linking agent and dimethyl sulfoxide (DMSO)-n-dodecyl alcohol system / water porogen solution, mix and stir, and ultrasonically induce solubilization for 10 minutes to form a mixed solution 1 of imprinted molecules and functional monomers;

[0051] B, the above-mentioned mixed solution-made is deoxygenated by nitrogen, and the nitrogen time is 10 minutes;

[0052] C, add 23.8mg initiator azobisisobutyronitrile in the mixed solution one after nitrogen deoxygenation, make the mixed solution two;

[0053] D. Pour the mixed solution II into a 100mm×4.6mm stainless steel chromatographic column tube I and seal it, and reac...

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Abstract

The invention discloses a method for preparing a molecularly imprinted monolithic column. Sulfamethoxazole (SMO) with the molecular structure similar to that of triamterene serves as a pseudo template molecule to prepare the molecularly imprinted monolithic column capable of achieving selective testing of triamterene (TAT).The problem that traditional template molecule leakage affects detection accuracy is solved.

Description

technical field [0001] The invention relates to the technical field of molecular imprinted chromatography, in particular to a method for preparing a molecularly imprinted monolithic column for the selective determination of triamterene. Background technique [0002] Molecular Imprinted Monolithic Column (Molecular Imprinted Monolithic Column) is a new type of chromatographic packing monolithic column that has developed rapidly in recent years. The chromatographic filler macroporous polymer medium is synthesized by in-situ polymerization method, that is, the solution of monomer, initiator, crosslinking agent and porogen is polymerized in an empty column. Molecularly imprinted monolithic column has the advantages of simple preparation process, fast mass transfer rate, good repeatability, low column pressure, etc. It is a kind of chromatographic stationary phase with great application potential, so it has received more and more attention. [0003] Molecularly imprinted monolit...

Claims

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Application Information

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IPC IPC(8): B01J20/281B01J20/30B01D15/22C08F222/14C08F220/06C08J9/28
CPCB01D15/22B01J20/281B01J2220/52B01J2220/54B01J2220/58B01J2220/82C08F222/1006C08J9/28C08J2335/02C08F222/102C08F220/06
Inventor 黄力彦李学斌陈大志谭艳来李恩如梁勇方建章孙创奇饶俊元李阳芬陈润发陈燕李诗瑶
Owner GUANGDONG ENG TECH INST
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