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Separation and purification method for puerarin

A technology of separation and purification, puerarin, applied in the direction of organic chemistry, etc., to achieve the effect of good elution effect and good washing effect

Inactive Publication Date: 2016-07-27
HUNAN UNIV OF SCI & ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But although the puerarin that above-mentioned these methods obtain is very high, the recovery rate is all below 70%, therefore, its recovery rate still needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Get Pueraria mirifica, pulverize after drying, pass through a 60-mesh sieve after pulverization, and the obtained under-sieve is used for reflux extraction;

[0034] The solvent of reflux extraction is that volume concentration is 80% ethanol aqueous solution, and reflux extracts altogether 3 times, and the volume of the used 80% ethanol aqueous solution of each reflux extraction is 8 times of Pueraria pueraria powder volume, and each extraction time is 3 hours, obtains extract ;

[0035] The extracts were combined, concentrated under reduced pressure to obtain the extract, the calculated extraction rate was 97%, and the puerarin content was about 11%;

[0036] Get medicinal extract, extract with organic solvent, and organic solvent is the mixed solution of water-saturated n-butanol and ammonium acetate, and the pH of mixed solution is adjusted to 4.5, and the addition of ammonium acetate is: the concentration of ammonium acetate in water-saturated n-butanol is 48g / L, ...

Embodiment 2

[0043] Get Pueraria mirifica, pulverize after drying, pass through a 60-mesh sieve after pulverization, and the obtained under-sieve is used for reflux extraction;

[0044] The solvent of reflux extraction is that volume concentration is 75% ethanol aqueous solution, reflux extracts altogether 4 times, the volume of the used 75% ethanol aqueous solution of each reflux extraction is 7 times of Pueraria pueraria powder volume, each extraction time is 2 hours, obtains extract ;

[0045] The extracts were combined, concentrated under reduced pressure to obtain the extract, the calculated extraction rate was 96.2%, and the puerarin content was about 11%;

[0046] Get medicinal extract, extract with organic solvent, organic solvent is the mixed solution of water-saturated n-butanol and ammonium acetate, the pH of mixed solution is adjusted to 4.4 with hydrochloric acid, the addition of ammonium acetate is: ammonium acetate in water-saturated n-butanol The concentration of puerarin ...

Embodiment 3

[0053] Get Pueraria mirifica, pulverize after drying, pass through a 60-mesh sieve after pulverization, and the obtained under-sieve is used for reflux extraction;

[0054] The solvent of reflux extraction is that the volume concentration is 85% ethanol aqueous solution, total reflux extraction 2 times, the volume of the used 85% ethanol aqueous solution of each reflux extraction is 9 times of Pueraria pueraria powder volume, each extraction time is 5 hours, obtains extract ;

[0055] The extracts were combined, concentrated under reduced pressure to obtain the extract, the calculated extraction rate was 96.5%, and the puerarin content was about 11%;

[0056] Get extract, extract with organic solvent, organic solvent is the mixed solution of water-saturated n-butanol and ammonium acetate, the pH of mixed solution is adjusted to 4.6 with hydrochloric acid, the addition of ammonium acetate is: ammonium acetate in water-saturated n-butanol The concentration of puerarin is 50g / L,...

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Abstract

The invention relates to the field of puerarin extraction, in particular to a separation and purification method for puerarin.The method includes the following steps that the root of kudzu vine is smashed, reflux extraction is conducted with ethanol water with the volume concentration of 80%+ / -5%, an obtained extracting solution is subjected to vacuum concentration, and extract is obtained; the extract is extracted, extract liquor adopted for extraction is mixed liquor of water-saturated n-butyl alcohol and ammonium acetate, the concentration of ammonium acetate in water-saturated n-butyl alcohol ranges from 46 g / L to 50 g / L, and pH of the mixed liquor ranges from 4.4 to 4.6; the extract obtained after extraction is diluted and then separated and purified with macroporous resin, eluant obtained after gradient elution is subjected to vacuum drying and recrystallization, and puerarin is obtained.According to the separation and purification method for puerarin, the purity of finally-obtained puerarin is 98% or above, and the recovery rate is 84% or above.

Description

technical field [0001] The invention relates to the field of puerarin extraction, in particular to a puerarin separation and purification method. Background technique [0002] Puerarin is a single-component flavonoid glycoside extracted from Pueraria mirifica, a leguminous plant. It has the functions of promoting blood circulation and removing blood stasis, improving microcirculation, dilating coronary arteries and cerebral blood vessels, and reducing myocardial oxygen consumption. Its preparation, puerarin injection, is clinically used It is used in the treatment of cardiovascular and cerebrovascular diseases, retinal vascular diseases, fundus diseases and sudden deafness. With the widespread clinical use of puerarin, the demand for puerarin is also increasing. [0003] There are many existing puerarin extraction methods. For example, the application number is 201410168838.1, which discloses a puerarin extraction process. Puerarin is crushed at -4 to 0°C and passed through...

Claims

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Application Information

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IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 邵金华何福林李玲陈小明刘小文姜红宇王宗成吕宏再张存根
Owner HUNAN UNIV OF SCI & ENG
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