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Bacterium and chondrosulphatase ABC generated by same

A chondroitinase sulfate, bacillus technology, applied in bacteria, microorganism-based methods, enzymes, etc., to achieve the effects of low cost, high product activity, and good stability

Active Publication Date: 2016-07-27
OCEAN UNIV OF CHINA
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  • Description
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Problems solved by technology

[0005] Through a large amount of information retrieval, at the same time comparing the molecular weight of enzyme-producing microorganisms and enzymes, no chondroitinase ABC identical to the inventor's research was found.

Method used

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  • Bacterium and chondrosulphatase ABC generated by same

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Embodiment Construction

[0022] Below in conjunction with specific example the present invention will be further described

[0023] 1. Screening and purification of an Acinetobacter sp.C26 strain

[0024] The acquisition of Acinetobacter sp.C26 bacterial strain of the present invention: collect the intertidal zone silt of east coast of Jiaodong, join in the enrichment medium that is equipped with 25mL (chondroitin sulfate 0.5g, yeast extract 5g, K 2 HPO 4 1g, MgSO 4 0.5g, NaCl15g, H 2 (O1000mL, pH7.2) Erlenmeyer flask, culture at 28°C, 160rpm shake flask for 2 days, then dilute the enriched culture solution 100 times with sterile saline, take 0.1mL and apply the primary screening separation medium (chondroitin sulfate 0.5 g, K 2 HPO 4 1g, MgSO 4 0.5g, yeast extract 5g, NaCl 15g, CaCl 2 0.01g, agar powder 18.0g, H 2 (O1000mL, natural pH) plate. Inverted at 28°C for 2 days, light yellow colonies with smooth edges on the selected plate were obtained, and a single colony was picked and cultured i...

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Abstract

The invention belongs to the biotechnical field, and provides a bacterium and chondrosulphatase ABC generated by same. The invention relates to acinetobacter (Acinetobacter sp.C26) from intertidal zone sludge. The bacterial strain has the characteristics of being simple in nutritional condition, easy to culture and short in generation time; and chondrosulphatase produced by the bacterial strain has the characteristics of being high in activity, good in stability and low in cost, can realize large-scale culture and meets industrial application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an Acinetobacter and the chondroitinase ABC produced therefrom. Background technique [0002] Chondroitinsulfate is an acidic mucopolysaccharide, which is a kind of macromolecular polysaccharide formed by the disaccharide units formed by glucuronic acid and hexosamine, which are connected alternately. It is widely distributed in the cartilage of animals and humans, such as animal Laryngeal bone, nasal bone, cartilage, sarcolemma and vessel wall, etc. There are many isomers of chondroitin sulfate. According to the different positions of the sulfate group in the hexosamine, it can be divided into chondroitin sulfate A (disaccharide unit: →4GlcAβ1, 3GalNAc4Sβ1→), chondroitin sulfate B ( Disaccharide unit: →4IdoAα1, 3GalNAc4Sβ1→), chondroitin sulfate C (disaccharide unit: →4GlcAβ1, 3GalNAc6Sβ1→), chondroitin sulfate D (disaccharide unit: →4GlcA2Sβ1, 3GalNAc6Sβ1→), etc. The ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/88C12R1/01
CPCC12N9/88C12Y402/0202C12Y402/02021C12N1/205C12R2001/01
Inventor 江晓路朱常亮
Owner OCEAN UNIV OF CHINA
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