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Modified antibacterial peptide and preparation of gel

A technology of peptide gel and antibacterial peptide, which is applied in the field of medicine, can solve the problems that have not been reported, and achieve the effects of good water solubility, resistance to bacteria, durability, and strong adhesion

Inactive Publication Date: 2016-08-03
SHANDONG INT BIOTECH PARK DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen that the modified antimicrobial peptide HC-17 of Qinghuan sea snake antimicrobial peptide Hc-CATH has a good application prospect, but there is no report on antimicrobial peptide HC-17 gel products.

Method used

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  • Modified antibacterial peptide and preparation of gel
  • Modified antibacterial peptide and preparation of gel
  • Modified antibacterial peptide and preparation of gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, a kind of antimicrobial peptide gel, its each component percentage content is respectively:

[0025]

[0026] The above raw materials are prepared according to the following method:

[0027] (1) Weigh Carbomer 940 powder according to the mass percentage of the raw materials, disperse it in an appropriate amount of purified water, soak it for 24 hours, and mix it evenly to obtain solution A;

[0028] (2) Weigh the antimicrobial peptide HC-17 powder and add it to an appropriate amount of purified water, perform ultrasonic treatment, and fully mix to obtain solution B;

[0029] (3) Azone and glycerin are mixed and added to solution A, and mixed to obtain solution C;

[0030] (4) Add solution B to solution C, make up the remaining purified water, stir and mix;

[0031] (5) Slowly add triethanolamine, stir evenly, adjust the pH to 6, the solution becomes jelly, and the antimicrobial peptide gel is obtained.

Embodiment 2

[0032] Embodiment 2, a kind of antimicrobial peptide gel, its each component percentage content is respectively:

[0033]

[0034] The above raw materials are prepared according to the following method:

[0035] (1) Weigh Carbomer 940 powder according to the mass percentage of the raw materials, disperse it in an appropriate amount of purified water, soak it for 24 hours, and mix it evenly to obtain solution A;

[0036] (2) Weigh the antimicrobial peptide HC-17 powder and add it to an appropriate amount of purified water, perform ultrasonic treatment, and fully mix to obtain solution B;

[0037] (3) Azone and propylene glycol are mixed and added to solution A, and mixed to obtain solution C;

[0038] (4) Add solution B to solution C, make up the remaining purified water, stir and mix;

[0039] (5) Slowly add sodium hydroxide, stir evenly, adjust the pH to 6, the solution becomes jelly, and the antimicrobial peptide gel is obtained.

Embodiment 3

[0040] Embodiment 3, a kind of antimicrobial peptide gel, its each component percentage content is respectively:

[0041]

[0042] The above raw materials are prepared according to the following method:

[0043] (1) Weigh Carbomer 940 powder according to the mass percentage of the raw materials, disperse it in an appropriate amount of purified water, soak it for 24 hours, and mix it evenly to obtain solution A;

[0044] (2) Weigh the antimicrobial peptide HC-17 powder and add it to an appropriate amount of purified water, perform ultrasonic treatment, and fully mix to obtain solution B;

[0045] (3) Azone and propylene glycol are mixed and added to solution A, and mixed to obtain solution C;

[0046] (4) Add solution B to solution C, make up the remaining purified water, stir and mix;

[0047] (5) Slowly add triethanolamine, stir evenly, adjust the pH to 6, the solution becomes jelly, and the antimicrobial peptide gel is obtained.

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Abstract

The invention belongs to the field of preparation of gel and specifically relates to modified antibacterial peptide HC-17 gel of Hydrophis cyanocinctus-derived antibacterial peptide Hc-CATH and a preparation method of the modified antibacterial peptide HC-17 gel. The preparation method comprises the following specific steps: (1) weighing a gel matrix according to mass percentage of raw materials, dispersing the gel matrix in a proper amount of purified water, immersing for 24h, and uniformly mixing to obtain a solution A; (2) weighing antibacterial peptide HC-17 and adding the antibacterial peptide HC-17 into a proper amount of purified water, and carrying out ultrasonic treatment to obtain a solution B; (3) uniformly mixing a skin penetration enhancer and a humectants and adding the mixture into the solution A, and uniformly mixing to obtain a solution C; (4) adding the solution B into the solution C, and complementing rest purified water; and (5) slowly adding a pH regulator, and uniformly stirring to obtain the antibacterial peptide gel. The product is safe and environmentally friendly, is nonirritating and is easy to coat and clean up. The technology of the invention is simple. The antibacterial peptide gel is a product with application potential.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an antimicrobial peptide HC-17 gel, which is a modified form of Qinghuan sea snake antibacterial peptide Hc-CATH, and a preparation method thereof. Background technique [0002] Cathelicidins are an important family of antimicrobial peptides in vertebrates with broad-spectrum and high-efficiency antimicrobial activity. The antibacterial peptides of the cathelicidins family that have been discovered so far have strong activity against Gram-positive bacteria, Gram-negative bacteria, fungi, molds, mycoplasma, chlamydia, protozoa and some enveloped viruses. [0003] Cathelicidins family antibacterial peptides have the characteristics of rapid bactericidal action and unique mechanism of action. By acting on the plasma membrane of microbial cells, a pore structure is formed on the plasma membrane, resulting in depolarization of the plasma membrane, changes in osmotic pressure inside and outside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61K9/06A61K47/32A61P31/04A61P31/10A61P33/02
Inventor 赵海婷张淑敏傅风华杨小平谢则平李东升宫世周
Owner SHANDONG INT BIOTECH PARK DEV
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