Sea cucumber fat-soluble extract, lip balm containing the extract and preparation method
A fat-soluble extract, extract technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve long-term problems, achieve low temperature, good biocompatibility, and simple preparation methods
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Embodiment 1
[0037] Embodiment 1: the extraction of sea cucumber fat-soluble extract
[0038]Rinse 1 kg of fresh sea cucumber with clean water quickly, cut open the abdomen, remove viscera, gonads, sediment, etc., take its body wall, wash the body wall with tap water and double distilled water respectively, cut it into pieces, soak in double distilled water for about 0.5h, then try to remove water, weighed. Add about 8 times the amount (mass ratio) of double distilled water to the shredded sea cucumber body wall, and use a disperser to homogenize to obtain a sea cucumber homogenate. Put the above sea cucumber homogenate in a constant temperature water bath at 50°C, adjust the pH to 2.0-2.5 with 6mol / L HCl solution, add a certain amount of pepsin according to 0.4% of the weight of the sea cucumber, then adjust the pH to 2.3, and bathe the enzyme in a constant temperature water bath at 50°C Solution for 5h, keep stirring, and keep the pH in the range of 2.0-2.5. After pepsin enzymatic hydr...
Embodiment 2
[0040] Embodiment 2: the extraction of sea cucumber fat-soluble extract
[0041] Rinse 5 kg of fresh sea cucumber with clean water quickly, cut open the abdomen, remove viscera, gonads, sediment, etc., take its body wall, wash the body wall with tap water and double distilled water respectively, cut it into pieces, soak in double distilled water for about 0.5h, then try to remove water, weighed. Add about 8 times the amount (mass ratio) of double distilled water to the shredded sea cucumber body wall, and use a disperser to homogenize to obtain a sea cucumber homogenate. Put the above sea cucumber homogenate in a constant temperature water bath at 50°C, adjust the pH to 2.0-2.5 with 6mol / L HCl solution, add a certain amount of pepsin according to 0.4% of the weight of the sea cucumber, then adjust the pH to 2.3, and bathe the enzyme in a constant temperature water bath at 50°C Solution for 5h, keep stirring, and keep the pH in the range of 2.0-2.5. After pepsin enzymatic hyd...
Embodiment 3
[0044] Rinse 5 kg of fresh sea cucumber with clean water quickly, cut open the abdomen, remove viscera, gonads, sediment, etc., take its body wall, wash the body wall with tap water and double distilled water respectively, cut it into pieces, soak in double distilled water for about 0.5h, then try to remove water, weighed. Add about 9 times the amount (mass ratio) of double distilled water to the shredded sea cucumber body wall, and use a disperser to homogenize to obtain a sea cucumber homogenate. Put the above sea cucumber homogenate in a constant temperature water bath at 50°C, adjust the pH to 2.0-2.5 with 6mol / L HCl solution, add a certain amount of pepsin according to 0.35% of the weight of the sea cucumber, and then adjust the pH to 2.3, and bathe the enzyme in a constant temperature water bath at 50°C Solution for 4.5h, keep stirring, and keep the pH in the range of 2.0-2.5. After pepsin enzymatic hydrolysis, put the enzymatic hydrolysis solution in a constant tempera...
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