Application of oridonin combined with curcumin in the preparation of drugs for treating leukemia
A technology of oridonin and curcumin, applied in the field of biomedicine, can solve the problems of retardation of proliferation, poor targeting, high cost, etc., achieve wide application prospects, and improve the effect of proliferation inhibition
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[0045] Example 1 Combination drug study of oridonin and curcumin
[0046] A certain concentration gradient of oridonin and curcumin were used to treat leukemia K562 cells for 24 hours, and at the same time, the concentration of the anticancer drug oridonin was fixed, and curcumin with different gradient concentrations was used to treat leukemia K562 cells for 24 hours. MTT colorimetric method was used to calculate the inhibitory intensity of cell proliferation under three different drug treatment modes. The specific research methods are as follows:
[0047] 1. Experimental cell lines
[0048] K562 chronic myelogenous leukemia cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).
[0049] 2. Cell culture:
[0050] K562 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture at a constant temperature of 37°C and 5% CO 2 conditions.
[0051] 3. Drug ...
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