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A strain for enhancing glycerol metabolism and its application

A technology of glycerol metabolism and bacterial strains, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of accumulation and inhibition of microbial cell growth, restore growth ability, change growth and metabolic performance, and achieve high efficiency The effect of using

Active Publication Date: 2019-07-26
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the reduction of the synthetic product is low (such as succinic acid), a large amount of NADH will be accumulated in the bacteria, and the excess reducing power will inhibit the growth of microorganisms

Method used

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  • A strain for enhancing glycerol metabolism and its application
  • A strain for enhancing glycerol metabolism and its application
  • A strain for enhancing glycerol metabolism and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example illustrates the construction method of the Actinobacillus succinogenes JF1315 strain of the present invention.

[0027] Actinobacillus succinate ( Actinobacillus succinogenes ) NJ113 strain has been disclosed in the patent previously applied by the same inventor, the patent number is ZL200610085415.9, and the preservation number is CGMCC No.1716.

[0028] Actinobacillus succinogenes ( Actinobacillus succinogenes ) JF1315 strain was produced by Actinobacillus succinogenes ( Actinobacillus succinogenes ) NJ113 was screened by ARTP mutagenesis.

[0029] The specific mutagenesis screening steps are as follows:

[0030] ARTP mutagenesis: the starting strain Actinobacillus succinogenes NJ113 was inoculated in an anaerobic serum bottle containing seed medium and cultured for 6-12 hours to obtain a strain in logarithmic growth phase. Properly dilute the seed culture solution to OD 660= 0.5-1.5 and then spread on the cooled slide for mutagenesis. The mutag...

Embodiment 2

[0037] This embodiment illustrates the physiological and biochemical characteristics of Actinobacillus succinogenes JF1315 obtained in the above-mentioned embodiment 1, specifically as follows:

[0038] Mutagenized strains and original bacteria Actinobacillus succinogenes There is no significant difference in the colony morphology and growth performance of NJ113: the strain is Gram-negative, and the plate colonies are round, with neat and smooth edges. Under anaerobic conditions, they can metabolize glucose and xylose to synthesize organic acids, and the main acid is succinic acid. , acetic acid, lactic acid and formic acid. The strain is glutamate-deficient and requires the addition of glutamate for growth on synthetic media.

[0039] The genetic stability test of Actinobacillus succinogenes JF1315 in Example 1, the strain subculture and fermentation experiments are as follows.

[0040] Mutagenized strain Actinobacillus succinogenes JF1315 was continuously subcultured ...

Embodiment 3

[0044] This embodiment illustrates the bacterial strain after mutagenesis in the present invention Actinobacillus succinogenes The superiority of JF1315 compared with the starting strain.

[0045] In the present invention, the Actinobacillus succinicum NJ113 and JF1315 strains are cultured on a solid plate medium and then inoculated into a seed medium for cultivation to obtain a seed liquid; then the seed liquid is inoculated into a low-pH fermentation medium for anaerobic fermentation. The method may include the steps of:

[0046] (1) Actinobacillus succinogenes NJ113 and JF1315 strains were activated on solid plate medium and then transferred to anaerobic serum bottle, cultured under anaerobic conditions at 37°C for 12-14 hours, then transferred to seed medium, at 37 Cultivate at 200 rpm for 6-8 hours to obtain seed liquid;

[0047] (2) Inoculate the above-mentioned seed liquid into a serum bottle containing a low pH fermentation medium (pH5.8) according to the inoculati...

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Abstract

The invention discloses a bacterial strain that strengthens glycerol metabolism, and the bacterial strain is classified and named as Actinobacillus succinate ( Actinobacillus succinogenes ) JF1315, which was deposited in China Center for Type Culture Collection on March 31, 2016, and its deposit number is: CCTCC NO: M 2016160. The present invention also relates to the application of the Actinobacillus succinogenes JF1315 in fermentative production of organic acids. Under the anaerobic condition, compared with the control strain, the strain can grow and metabolize at a lower pH condition, and can efficiently utilize glycerol to ferment and synthesize reduced organic acids. Under the condition of pH 4.8-6.8, the strain can grow normally, and metabolize glucose to synthesize organic acids such as succinic acid; in addition, under this condition, the strain can efficiently use glycerol for anaerobic fermentation, synthesize and accumulate organic acids. acid.

Description

technical field [0001] The invention relates to a bacterial strain that strengthens glycerol metabolism and its application, belonging to the technical field of industrial microbes and their fermentation. Background technique [0002] In the traditional engineering practice in the field of microbial fermentation, carbohydrates are generally used as carbon sources for fermentation, such as corn starch hydrolyzate; for some high value-added products, glucose is even directly used as raw material for product synthesis . Although a product with a higher concentration can be obtained when using raw materials such as glucose for fermentation, this greatly increases the economic cost of the entire production process and causes a waste of resources. In order to solve this problem, cheap biomass materials (such as cellulose hydrolyzate or glycerol) can be tried to replace glucose for fermentation, but the existence of toxic substances and low sugar concentration in cellulose hydroly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/46C12P7/40C12R1/01
CPCC12P7/40C12P7/46C12N1/205C12R2001/01
Inventor 姜岷冀亚亮马江锋陈美丽
Owner NANJING TECH UNIV
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