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Method for preparing ACE inhibitory peptide through enzymolysis of bromelain on konjak protein

The technology of bromelain and inhibitory peptides is applied in the field of preparation of ACE inhibitory peptides, which can solve the problems of low utilization value, achieve the effects of low cost, widen development and utilization fields, and facilitate mass production

Inactive Publication Date: 2016-08-10
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Konjac powder contains about 20% protein. At present, the utilization of konjac is mainly concentrated on konjac fine powder (glucomannan), and konjac powder is mostly sold as feed or desiccant at a low price, and its utilization value is low.

Method used

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  • Method for preparing ACE inhibitory peptide through enzymolysis of bromelain on konjak protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) konjac protein is dissolved in pure water to be configured as a konjac protein solution with a mass fraction of 1%;

[0034] (2) After adjusting the pH value with 1.0moL / L NaOH solution, add pineapple white enzyme and mix evenly, and carry out enzymolysis reaction on a constant temperature magnetic stirrer to obtain an enzymolysis solution; wherein, the enzymolysis conditions are: the pH value is 4.0, Substrate (being konjac protein solution) mass fraction is 1%, and enzyme amount is 3000U / g (promptly adding the amount of pineapple white enzyme in every gram of konjac protein solution is 3000U), enzymolysis time is 1h, and enzymolysis temperature is 40°C;

[0035] (3) Place the enzymatic hydrolysis solution in a constant temperature water bath at 100°C to inactivate the enzyme for 10 minutes;

[0036] (4) After the temperature of the enzymolysis solution drops to room temperature, adjust its pH value to the isoelectric point of konjac protein, then centrifuge at 90...

Embodiment 2

[0039] (1) konjac protein is dissolved in pure water to be configured as a konjac protein solution with a mass fraction of 2%;

[0040] (2) After adjusting the pH value with 1.0moL / L NaOH solution, add pineapple white enzyme and mix evenly, and carry out enzymolysis reaction in a constant temperature magnetic stirrer to obtain an enzymolysis solution; wherein, the enzymolysis conditions are: pH value is 5.0, bottom The mass fraction of the substance is 2%, the amount of enzyme added is 3500U / g (that is, the amount of pineapple white enzyme added to each gram of konjac protein solution is 3500U), the enzymolysis time is 2h, and the enzymolysis temperature is 50°C;

[0041] (3) Put the enzymatic solution in a constant temperature water bath at 100°C to inactivate the enzyme for 15 minutes;

[0042] (4) After the temperature of the enzymolysis solution drops to room temperature, adjust its pH value to the isoelectric point of konjac protein, then centrifuge at 9500r / min for 15min...

Embodiment 3

[0045] (1) konjac protein is dissolved in pure water to be configured as a konjac protein solution with a mass fraction of 3%;

[0046] (2) After adjusting the pH value with 1.0moL / L NaOH solution, add pineapple white enzyme and mix evenly, and carry out enzymolysis reaction in a constant temperature magnetic stirrer to obtain an enzymolysis solution; wherein, the enzymolysis conditions are: pH value is 6.0, bottom The mass fraction of the substance is 3%, the amount of enzyme added is 4000U / g (that is, the amount of pineapple white enzyme added to each gram of konjac protein solution is 4000U), the enzymolysis time is 3h, and the enzymolysis temperature is 55°C;

[0047] (3) Place the enzymatic hydrolysis solution in a constant temperature water bath at 100°C to inactivate the enzyme for 10 minutes;

[0048] (4) After the temperature of the enzymolysis solution drops to room temperature, adjust its pH value to the isoelectric point of konjac protein, then centrifuge at 10000r...

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Abstract

The invention discloses a method for preparing ACE inhibitory peptide through enzymolysis of bromelain on konjak protein. The konjak protein serves as a raw material, bromelain is used for conducting enzymolysis, enzymolysis is conducted for a while under a certain enzymolysis condition, enzymatic hydrolysate is obtained through enzyme deactivation, cooling, pH value adjusting and centrifugation, high-purity ACE inhibitory liquid is obtained after the enzymatic hydrolysate is subjected to ultrafiltration, SephadexG-25 gel chromatography and RT-HPLC separation and purification, and by means of cooling and drying, konjak ACE inhibitory peptide powder is obtained. The konjak powder is effectively utilized, and the method has important significance in producing high-value-added products of the konjak powder.

Description

technical field [0001] The invention belongs to the field of preparation of ACE inhibitory peptides, and in particular relates to a method for preparing ACE inhibitory peptides by enzymolyzing konjac protein with bromelain. Background technique [0002] The national annual output of konjac powder is 1500-2000 tons. Konjac flour contains about 20% protein. At present, the utilization of konjac is mainly concentrated on konjac powder (glucomannan), and konjac flour is mostly sold as feed or desiccant at a low price, and its utilization value is low. [0003] Hypertension is a chronic disease characterized by continuous elevation of arterial blood pressure, which often causes lesions in heart, brain, kidney and other important organs with corresponding consequences. Hypertensive patients generally need to take antihypertensive drugs for a long time, which may lead to adverse reactions such as cough, decreased renal function, proteinuria, abnormal liver function, and angioneuro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 毛跟年周亚丽贺磊曹晴许牡丹
Owner SHAANXI UNIV OF SCI & TECH
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