Preparation method of Chinese chestnut functional fermented food
A fermented food and functional technology, applied in the fields of microbial fermentation and biomedicine, to achieve the effects of promoting growth and reproduction, invigorating the kidney and strengthening the gastrointestinal tract function, and improving the gastrointestinal tract function.
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Embodiment 1
[0026] A preparation method of chestnut functional fermented food is prepared by the following steps:
[0027] (1) Chestnut fermentation medium:
[0028] Wash the chestnuts, add 0.02 kg of ammonium chloride, 0.5 kg of maltose, 0.05 kg of magnesium sulfate, 0.01 kg of dipotassium hydrogen phosphate, and 100 L of water for every 100 kg of chestnuts;
[0029] (2) Monascus liquid strains:
[0030] After the Monascus strain was activated and cultured on a malt juice agar slant medium for 5 days, it was inserted into a liquid shake flask medium for liquid fermentation culture to prepare a liquid strain of Monascus;
[0031] (3) Lactic acid bacteria liquid strains:
[0032] After the lactic acid bacteria strains are anaerobically activated and cultivated on the MRS agar slant medium for 1 day, they are inserted into the liquid lactic acid bacteria expansion medium for liquid anaerobic fermentation culture to prepare lactic acid bacteria liquid strains;
[0033] (4) Preparation of ...
Embodiment 2
[0045] A preparation method of chestnut functional fermented food is prepared by the following steps:
[0046] (1) Chestnut fermentation medium:
[0047] Wash the chestnuts, add 0.02 kg of ammonium chloride, 0.5 kg of maltose, 0.05 kg of magnesium sulfate, 0.01 kg of dipotassium hydrogen phosphate, and 100 L of water for every 100 kg of chestnuts;
[0048] (2) Monascus liquid strains:
[0049] After the Monascus strain was activated and cultured on a malt juice agar slant medium for 5 days, it was inserted into a liquid shake flask medium for liquid fermentation culture to prepare a liquid strain of Monascus;
[0050] (3) Lactic acid bacteria liquid strains:
[0051] After the lactic acid bacteria strains are anaerobically activated and cultivated on the MRS agar slant medium for 1 day, they are inserted into the liquid lactic acid bacteria expansion medium for liquid anaerobic fermentation culture to prepare lactic acid bacteria liquid strains;
[0052] (4) Preparation of ...
Embodiment 3
[0064] A preparation method of chestnut functional fermented food is prepared by the following steps:
[0065] (1) Chestnut fermentation medium:
[0066] Wash the chestnuts, add 0.02 kg of ammonium chloride, 0.5 kg of maltose, 0.05 kg of magnesium sulfate, 0.01 kg of dipotassium hydrogen phosphate, and 100 L of water for every 100 kg of chestnuts;
[0067] (2) Monascus liquid strains:
[0068] After the Monascus strain was activated and cultured on a malt juice agar slant medium for 5 days, it was inserted into a liquid shake flask medium for liquid fermentation culture to prepare a liquid strain of Monascus;
[0069] (3) Lactic acid bacteria liquid strains:
[0070] After the lactic acid bacteria strains are anaerobically activated and cultivated on the MRS agar slant medium for 1 day, they are inserted into the liquid lactic acid bacteria expansion medium for liquid anaerobic fermentation culture to prepare lactic acid bacteria liquid strains;
[0071] (4) Preparation of ...
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