Immunochromatographic detection test strip for joint detection of NSE and CEA, and preparation method and application method thereof

Abstract

The invention discloses an immunochromatographic detection test strip for combined detection of NSE and CEA, which adopts a double-antibody sandwich method, and includes a backing plate, a sample pad, a binding pad, and a chromatography pad laid on the backing plate in sequence overlapping each other. pad, water-absorbing pad; the first nano-magnetic bead immune probe and the second nano-magnetic bead immune probe are arranged on the binding pad; the first nano-magnetic bead immune probe includes carboxyl nano-magnetic beads and anti-NSE monoclonal antibody, and the second The nano-magnetic bead immunoprobe includes carboxyl nano-magnetic beads and anti-CEA monoclonal antibody; the chromatography pad is provided with a first detection line, a second detection line and a quality control line in sequence, wherein the quality control line is compared with the first detection line Closer to the absorbent pad; the first detection line is equipped with mouse NSE detection antibody, the second detection line is equipped with mouse CEA detection antibody; the quality control line is equipped with goat anti-mouse secondary antibody. The invention can greatly improve the sensitivity and accuracy of detecting NSE and CEA.

Description

technical field [0001] The invention relates to a test strip and a preparation method and an application method thereof, in particular to an immunochromatographic detection test strip for joint detection of NSE and CEA, a preparation method and an application method thereof. Background technique [0002] Near-immune chromatography technology is a new type of detection technology that combines immunolabeling technology and chromatography technology. It can use the chromogenic properties of markers to achieve qualitative, semi-quantitative, and quantitative analysis of the analyte. It uses the microporous filter membrane as the solid phase carrier, the liquid to be tested as the mobile phase, and uses the capillary action and siphon effect of the microporous filter membrane to guide the liquid to be tested to flow forward. The antigen or antibody on the filter membrane reacts to form an immune complex, which stays on the detection line. Through the optical density analysis of ...

AI Technical Summary

Problems solved by technology

[0009] 1. Traditional enzyme-linked immunosorbent assay and mature immunochromatographic detection can only detect one marker at a time, which cannot meet the needs of simultaneous detection of multiple markers

[0010] 2. The existing detection technology, because the detection results can only be judged by visual inspection, is very subjective; therefore, only qualitative detection, semi-quantitative or inaccurate quantitative detection can be realized; the detection sensitivity of immunochromatography also needs to be improved

[0011] 3. Quantitative immunoassay technology based on photoelectric effect can only detect the light of immune particles on the surface of nitrocellulose membrane, so it is difficult to truly reflect the total amount of immune particles on the detection line, which is not conducive to the accuracy of quantitative detection

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