Immunochromatographic test paper strip, portable detection instrument and detection method

Abstract

The invention provides an immunochromatographic test paper strip for quantitatively detecting aflatoxin M1. The immunochromatographic test paper strip comprises a sample binding region and a detection line, wherein the sample binding region is sprayed with aflatoxin Ml monoclonal antibodies marked by quantum dots; the detection line is coated with an aflatoxin Ml antigen / hapten-carrier protein conjugate. The light emission wavelength of each of the aflatoxin Ml monoclonal antibodies marked by the quantum dots is 520-650 nm; each of the quantum dots is at most coupled with the two aflatoxin Ml monoclonal antibodies. The invention further provides a portable detection instrument which comprises a light source module and a display module and cooperates with the immunochromatographic test paper strip to quantitatively detect the aflatoxin Ml. In addition, the invention further provides a method for detecting the aflatoxin Ml by using the immunochromatographic test paper strip and the portable detection instrument.

Description

technical field [0001] The invention relates to the field of food detection, in particular to an immunochromatographic test strip, a portable detector and a detection method for rapid and quantitative detection of aflatoxin M1 by using quantum dot fluorescence immunochromatography on the basis of preferred materials. Background technique [0002] Aflatoxins are a group of compounds with similar chemical structures and the same toxic groups produced by the metabolism of certain molds (such as Aspergillus flavus and Aspergillus parasiticus), often found in improperly stored peanuts, corn, rice, wheat, sorghum, sunflower Found in bulk commodities such as seeds, nuts, cotton seeds, etc. At present, more than 20 kinds of aflatoxins have been isolated and identified. Among them, there are mainly aflatoxins B1, B2, G1, G2 and the metabolites M1 and M2 derived from the hydroxylation of B1 and B2 in vivo. Aflatoxins B1 and M1 are carcinogenic, teratogenic, and mutagenic toxic compou...

AI Technical Summary

Problems solved by technology

The quantum dots synthesized in organic systems have almost perfect crystal structure, good monodispersity, strong photostability and high fluorescence yield, but tributylphosphine, trioctyl Organic phosphine compounds such as phosphine are highly toxic, flammable, unstable, explosive, expensive, and have high requirements for the operating environment, which limits the large-scale production and application of quantum dot products

In addition, in the process of preparing quantum dot fluorescent probes, the coupling products of quantum dots and corresponding antibody molecules have poor uniformity and dispersion, are prone to agglomeration, low luminous efficiency, and the half-peak width of the emission spectrum is too broad, etc. Problems that affect the detection performance of related products

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