Quadruple fluorescent pcr rapid ultrasensitive detection kit for b19, htlv and hev and its application

A kit and re-detection technology, applied in the field of multiplex real-time PCR, can solve problems such as the inability to design quadruple non-interfering primer pairs and probes, distortion, and no prompting for probe concentration adjustments, etc.
CN105886665BActive Publication Date: 2019-06-28苏州华益美生物科技有限公司
11 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
苏州华益美生物科技有限公司
Publication Date
2019-06-28

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a B19 (Human parvovirus B19), HTLV (Human T-cell lymphotropic virus) and HEV (Hepatitis E virus) quadruple fluorescent PCR (polymerase chain reaction) quick hypersensitivity detection kit and an application thereof, and relates to a real-time PCR method for quadruple detection of target nucleic acid in a nucleic acid extraction liquid in a single PCR reaction container. The method is used for detecting B19, HTLV-I, HTLV-2 and HEV.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of nucleic acid detection. Specifically, the invention relates to the ability to simultaneously detect human parvovirus B19 (Human parvovirus B19, B19), human T-cell lymphotropic virus type I (Human T-cell lymphotropic virus 1, HTLV-I), A multiplex real-time PCR method for detecting four pathogenic pathogens of human T-cell lymphotropic virus 2 (HTLV-II) and hepatitis E virus (HEV), which is fast and ultrasensitive And complete in one step. In addition, the present invention also relates to the reagents involved in the above PCR method, such as primers, probes, etc. that are irrelevant to each other, as well as the detection kit used in the above method and the preparation and application of the corresponding detection kit. Background technique

[0002] Human parvovirus B19 (Human parvovirus B19) is a linear single-stranded DNA virus with a genome size of 5.6 kb. In 1975, when Cossart et al. detected the h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More