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Metabonomics analysis method for biological fermentation feed

A technology of biological fermentation feed and metabolomics, which is applied in the direction of material analysis by resonance, material analysis, and nuclear magnetic resonance analysis, etc. efficiency, improve quality, and simplify the extraction process

Inactive Publication Date: 2016-08-24
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

However, the hot acetonitrile extraction method will cause the loss of volatile small molecular substances due to boiling at 70°C for 5 minutes, resulting in low extraction efficiency and even chemical reactions of amino acids and sugars.
The extraction efficiency of the 50% methanol method (-20°C) is not as high as that of the acidic methanol-acetonitrile method (-20°C).
However, in general, due to the temperature of the extraction process and the polarity of the extraction reagents, the detection efficiency varies greatly
Moreover, reagents such as methanol and acetonitrile have the disadvantages of high toxicity, cumbersome operation, long time consumption, and large environmental pollution.
Although the concentration of palmitic acid, stearic acid, oleic acid and linoleic acid can be determined simultaneously, short-chain fatty acids such as formic acid, acetic acid, propionic acid and butyric acid cannot be detected at the same time, nor can amino acids and other compounds be detected simultaneously

Method used

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  • Metabonomics analysis method for biological fermentation feed
  • Metabonomics analysis method for biological fermentation feed
  • Metabonomics analysis method for biological fermentation feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Experimental method

[0032] 1.1 Sample

[0033] Sorghum-based wine tank fermented by Bacillus subtilis

[0034] 1.2 Experimental equipment

[0035] Analytical balance (Sartorius BS110S), Sartorius Company, Germany; micro-sampler, Thermo Company, USA; acidity meter (HANNA pH 211 type), HANNA Company, Italy; vortex oscillator (SI-Vortex-Genie 2 type), Scientific Industries Company, USA ; Low-temperature refrigerated large-capacity centrifuge (Anke DL-4000B type), Shanghai Anting Scientific Instrument Factory; ultra-low temperature refrigerator, Qingdao Haier Company; Bruker Avance DRX-600 high-resolution nuclear magnetic resonance spectrometer, Germany Bruker Biospin Company.

[0036] 1.3 The sample extraction method of the present invention is as follows:

[0037] Get about 20g of thawed or fresh bio-fermented feed samples, fully crushed (all through a 20 mesh sieve), then weigh 0.1g of bio-fermented feed samples, add 0.2M phosphate buffer (pH value 7.4, 0.002%TSP...

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Abstract

The invention discloses a metabonomics analysis method for biological fermentation feed. By means of the method, the metabolin extraction rate is improved, the quality of nuclear magnetic spectra is improved, the extraction process is simplified, metabonomics analysis for the biological fermentation feed is achieved, and the biological fermentation feed is well distinguished from raw materials.

Description

technical field [0001] The invention belongs to the technical field of metabolomics. In particular, it relates to a metabolomics analysis method for biologically fermented feed. Background technique [0002] Bio-fermented feed is currently a hot spot in research and development. It has shown great prospects in many aspects such as ensuring the safety of feed resources and livestock products, promoting emission reduction, and reducing environmental pollution. The development of bio-fermented feed has great strategic significance. Bio-fermented feed has been widely used in foreign countries, and the overall research level is higher than that in China, especially in the physiological and biochemical aspects of feed bioactive additives. In addition to a few traditional fermented products, the research on biological feed in my country generally has problems such as insufficient production technology, few varieties, and unmatched application technology. However, at present, both...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/088
Inventor 何庆华任萍萍李铁军孔祥峰印遇龙伍力崔志杰李凤娜刘莹莹
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI