Metabonomics analysis method for biological fermentation feed

Abstract

The invention discloses a metabonomics analysis method for biological fermentation feed. By means of the method, the metabolin extraction rate is improved, the quality of nuclear magnetic spectra is improved, the extraction process is simplified, metabonomics analysis for the biological fermentation feed is achieved, and the biological fermentation feed is well distinguished from raw materials.

Description

technical field [0001] The invention belongs to the technical field of metabolomics. In particular, it relates to a metabolomics analysis method for biologically fermented feed. Background technique [0002] Bio-fermented feed is currently a hot spot in research and development. It has shown great prospects in many aspects such as ensuring the safety of feed resources and livestock products, promoting emission reduction, and reducing environmental pollution. The development of bio-fermented feed has great strategic significance. Bio-fermented feed has been widely used in foreign countries, and the overall research level is higher than that in China, especially in the physiological and biochemical aspects of feed bioactive additives. In addition to a few traditional fermented products, the research on biological feed in my country generally has problems such as insufficient production technology, few varieties, and unmatched application technology. However, at present, both...

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Problems solved by technology

However, the hot acetonitrile extraction method will cause the loss of volatile small molecular substances due to boiling at 70°C for 5 minutes, resulting in low extraction efficiency and even chemical reactions of amino acids and sugars.

The extraction efficiency of the 50% methanol method (-20°C) is not as high as that of the acidic methanol-acetonitrile method (-20°C).

However, in general, due to the temperature of the extraction process and the polarity of the extraction reagents, the detection efficiency varies greatly

Moreover, reagents such as methanol and acetonitrile have the disadvantages of high toxicity, cumbersome operation, long time consumption, and large environmental pollution.

Although the concentration of palmitic acid, stearic acid, oleic acid and linoleic acid can be determined simultaneously, short-chain fatty acids such as formic acid, acetic acid, propionic acid and butyric acid cannot be detected at the same time, nor can amino acids and other compounds be detected simultaneously

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