Preparation of noble metal nanocluster visualization reagent and method for visualization of latent sweat fingerprints
A nano-cluster, precious metal technology, applied in metal processing equipment, chemical instruments and methods, sensors, etc., can solve the problems of low synthesis efficiency, short fluorescence duration, and long time-consuming of gold nano-clusters
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Embodiment 1
[0098] A preparation method of a precious metal nano-cluster visualization reagent of the present invention comprises the following steps: dissolving 1% chloroauric acid in pure water to form a 0.01 mol / L solution; dissolving bovine serum albumin in pure Water was prepared as a 50 mg / mL solution; potassium hydroxide was dissolved in pure water to form a 1 mol / L solution; the above prepared chloroauric acid solution and bovine serum albumin solution were mixed at a volume ratio of 1:1, placed in React under a magnetic stirrer for 3 minutes; use an infusion needle to slowly add potassium hydroxide solution dropwise to the mixed solution of chloroauric acid solution and bovine serum albumin solution described in this step, and adjust the pH to 12. At this time, the 0.01mol / The volume ratio of 1 L of chloroauric acid solution, 50 mg / mL bovine serum albumin solution, and 1 mol / L potassium hydroxide solution is 10:10:1; the mixed solution with a pH of 12 is first placed in an ultras...
Embodiment 2
[0100] A preparation method of a precious metal nano-cluster visualization reagent of the present invention comprises the following steps: dissolving 1% silver nitrate in pure water to form a 0.01 mol / L solution; dissolving bovine serum albumin in pure water at 37°C Prepare a solution; dissolve potassium hydroxide in pure water to form a 1 mol / L solution; mix the silver nitrate solution and bovine serum albumin solution prepared above at a volume ratio of 1:1, and place them under a magnetic stirrer for 3 minutes Slowly drip potassium hydroxide solution into the mixed solution of silver nitrate solution and bovine serum albumin solution described in this step with an infusion needle, adjust the pH to be 12, the silver nitrate solution of 0.01 mol / L used at this moment, 50 The volume ratio of mg / mL bovine serum albumin solution and 1 mol / L potassium hydroxide solution is 10:10:1; put the mixed solution with pH 12 in an ultrasonic washing machine for 1 hour; then put it in a hous...
Embodiment 3
[0102] A preparation method of a precious metal nano-cluster visualization reagent of the present invention comprises the following steps: dissolving 1% chloroauric acid in pure water to form a 0.01 mol / L solution; dissolving 1% silver nitrate in pure water to form a 0.01 mol / L solution; mol / L solution; dissolving bovine serum albumin in pure water at 37°C to form a solution; dissolving potassium hydroxide in pure water to form a 1 mol / L solution; Silver nitrate solution and bovine serum albumin solution were mixed at a volume ratio of 1:1:2, and placed under a magnetic stirrer to react for 3 minutes; the potassium hydroxide solution was slowly added dropwise to the above mixed solution with an infusion needle, and the pH was adjusted to 12, The chloroauric acid solution of 0.01 mol / L used at this moment, the silver nitrate solution of 0.01 mol / L, the bovine serum albumin solution of 50 mg / mL, the potassium hydroxide solution volume ratio of 1 mol / L are 5:5: 10:1; the mixed so...
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