Preparation method of photosynthetic bacterium solid medium

Abstract

The invention relates to a preparation method of a photosynthetic bacterium solid medium, and belongs to the technical field of medium preparation. The preparation method mainly includes the steps that mistletoe leaves are pretreated, stir-fried and ground, and mistletoe leaf powder is obtained; then, the mistletoe leaf powder, Prunus persica (L.) Batsch and liquid supernatant in a biogas digester are stirred, mixed and fermented, and mixed fermentation liquor is obtained; then, the mixed fermentation liquor and substances such as sodium acetate and ammonium chloride are stirred and mixed, the mixture is sterilized, and the photosynthetic bacterium solid medium is obtained. According to the preparation method, the mistletoe leaves are fully utilized for fermentation, so that the photosynthetic bacterium solid medium is loose and porous, and the problems that the production process is difficult to control and large-scale production is difficult to achieve in a shallow-layer or thin-layer fermentation process are avoided; besides, transportation is convenient, packaging is easy, and cost is lowered.

Description

technical field [0001] The invention relates to a method for preparing a solid medium for photosynthetic bacteria, belonging to the technical field of medium preparation. Background technique [0002] Photosynthetic bacteria is a general term for a large class of prokaryotic organisms that can perform non-oxygenous photosynthesis under anaerobic light conditions. There are 4 families in the family, and the fungal cells are mainly divided into two different shapes: rod-shaped and spherical. It is one of the pioneer species of the origin of life on earth. Among them, Rhodopseudomonas and Violet Sulfur-free bacteria are the photosynthetic bacteria species with more industrial production. The protein content of photosynthetic bacteria is as high as more than 60%. At the same time, it is rich in coenzyme Q10 (ubiquinone) that can be used as a cell metabolism activator and natural antioxidant, and carotenoids with anticancer effects. It has important nutritional and medicinal val...

AI Technical Summary

Problems solved by technology

[0003] Most of the products currently used are liquid preparations prepared by liquid fermentation process. Liquid preparations have a quick effect, but they are basically released in the form of original culture fluid. The effective bacteria amount in a unit liquid preparation is relatively low, about 109cell / ml. The product is bulky, the cost of the packaging container is high, and the transportation cost is high

Moreover, the production of high-quality liquid preparations has high requirements for production equipment and filling equipment. The aseptic operation must be controlled throughout the production process, and the culture medium and culture equipment need to be thoroughly sterilized. Filling also requires expensive equipment and high requirements. Sterile environment, high investment and production costs

To overcome this problem, solid fermentation can be used. However, due to the limitation of light, solid fermentation generally adopts shallow or thin layer fermentation technology and drying to prepare products. The preparation is stable and easy to store and transport, but the production process is difficult to control and difficult to achieve scale. change