Preparation method of photosynthetic bacterium solid medium

A technology of solid medium and photosynthetic bacteria, applied in the direction of bacteria, etc., can solve the problems of high investment and production costs, difficult control of the production process, high cost of packaging containers, etc., and achieve the effects of reducing production costs, simple production process, and convenient transportation

Inactive Publication Date: 2016-08-31
郭舒洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the products currently used are liquid preparations prepared by liquid fermentation process. Liquid preparations have a quick effect, but they are basically released in the form of original culture fluid. The effective bacteria amount in a unit liquid preparation is relatively low, about 109cell / ml. The product is bulky, the cost of the packaging container is high, and the transportation cost is high
Moreover, the production of high-quality liquid preparations has high requirements for production equipment and filling equipment. The aseptic operation must be controlled throughout the production process, and the culture medium and culture equipment need to be thoroughly sterilized. Filling also requires expensive equipment and high requirements. Sterile environment, high investment and production costs
To overcome this problem, solid fermentation can be used. However, due to the limitation of light, solid fermentation generally adopts shallow or thin layer fermentation technology and drying to prepare products. The preparation is stable and easy to store and transport, but the production process is difficult to control and difficult to achieve scale. change

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First weigh 500g of fresh mistletoe leaves, after manual removal of impurities, wash with deionized water 5 times, then evenly spread it on a pineapple sieve in a dark and ventilated place, the laying thickness is 4cm, lightly turn it every 2h , air-dried for 10 hours; after the leaves of mistletoe were air-dried, put them into an iron pan, heat to 160°C, stir-fry quickly for 4 minutes, stop heating, continue to stir-fry for 10 minutes using the residual temperature of the iron pan, and discharge the material. Spread mistletoe leaves evenly on a stone mill, and grind for 8 minutes to obtain mistletoe leaf powder; then add 160 g of mistletoe leaf powder obtained above, 30 g of peach gum and 25 mL of For the supernatant of the biogas tank, start the agitator, stir and mix at a speed of 300r / min for 8 minutes, stop stirring, heat up to 40°C, and carry out anaerobic fermentation at a constant temperature for 8 hours. 5min, discharge, and naturally cool to room temperature t...

example 2

[0019] First, weigh 300g of fresh mistletoe leaves, after manual removal of impurities, wash with deionized water for 3 times, then evenly spread it on a pineapple sieve in a dark and ventilated place, the laying thickness is 2cm, and lightly turn it every 1h , air-dried for 8 hours; after the leaves of mistletoe were air-dried, put them into an iron pot, heat to 140°C, stir-fry quickly for 3 minutes, stop heating, use the remaining temperature of the iron pot to continue stir-fry for 7 minutes, and discharge the material. Spread mistletoe leaves evenly on a stone mill, and grind for 6 minutes to obtain mistletoe leaf powder; then add 120 g of mistletoe leaf powder obtained above, 20 g of peach gum and 20 mL of For the supernatant of the biogas tank, start the agitator, stir and mix at 200r / min for 5 minutes, stop stirring, heat up to 35°C, and carry out anaerobic fermentation at a constant temperature for 6 hours. 3min, discharge, and naturally cool to room temperature to obt...

example 3

[0022]First, weigh 400g of fresh mistletoe leaves, after manual removal of impurities, wash with deionized water 4 times, and then evenly spread it on a pineapple sieve in a dark and ventilated place, laying a thickness of 3cm, turning it lightly every 1h , air-dried for 9 hours; after the leaves of mistletoe were air-dried, put them into an iron pot, heat to 150°C, stir-fry quickly for 4 minutes, stop heating, use the remaining temperature of the iron pot to continue to stir-fry for 8 minutes, discharge the material, and stir-fry the Spread mistletoe leaves evenly on a stone mill, grind for 7 minutes to obtain mistletoe leaf powder; then add 130 g of mistletoe leaf powder obtained above, 25 g of peach gum and 22 mL of For the supernatant of the biogas tank, start the agitator, stir and mix at a speed of 250r / min for 7 minutes, stop stirring, heat up to 37°C, and carry out anaerobic fermentation at a constant temperature for 7 hours. 4min, discharge, and naturally cool to room...

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Abstract

The invention relates to a preparation method of a photosynthetic bacterium solid medium, and belongs to the technical field of medium preparation. The preparation method mainly includes the steps that mistletoe leaves are pretreated, stir-fried and ground, and mistletoe leaf powder is obtained; then, the mistletoe leaf powder, Prunus persica (L.) Batsch and liquid supernatant in a biogas digester are stirred, mixed and fermented, and mixed fermentation liquor is obtained; then, the mixed fermentation liquor and substances such as sodium acetate and ammonium chloride are stirred and mixed, the mixture is sterilized, and the photosynthetic bacterium solid medium is obtained. According to the preparation method, the mistletoe leaves are fully utilized for fermentation, so that the photosynthetic bacterium solid medium is loose and porous, and the problems that the production process is difficult to control and large-scale production is difficult to achieve in a shallow-layer or thin-layer fermentation process are avoided; besides, transportation is convenient, packaging is easy, and cost is lowered.

Description

technical field [0001] The invention relates to a method for preparing a solid medium for photosynthetic bacteria, belonging to the technical field of medium preparation. Background technique [0002] Photosynthetic bacteria is a general term for a large class of prokaryotic organisms that can perform non-oxygenous photosynthesis under anaerobic light conditions. There are 4 families in the family, and the fungal cells are mainly divided into two different shapes: rod-shaped and spherical. It is one of the pioneer species of the origin of life on earth. Among them, Rhodopseudomonas and Violet Sulfur-free bacteria are the photosynthetic bacteria species with more industrial production. The protein content of photosynthetic bacteria is as high as more than 60%. At the same time, it is rich in coenzyme Q10 (ubiquinone) that can be used as a cell metabolism activator and natural antioxidant, and carotenoids with anticancer effects. It has important nutritional and medicinal val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 郭舒洋薛培龙许博伟
Owner 郭舒洋
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