Tissue culture rapid propagation method for moonlight jujubes

A tissue culture rapid propagation and moonlight technology, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of different jujube tissue culture methods, large differences in breeding conditions, and difficulty in jujube tissue culture. Accelerate the rapid promotion and development and utilization, increase the reproduction speed, and facilitate the effect of plant utilization

Active Publication Date: 2016-09-07
象州县科学技术局
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

So far, people have made some achievements in the study of jujube tissue culture, and the technology of using tissue culture to propagate fine varieties of jujube has been gradually applied to production. However, the tissue culture of jujube is difficult and expensive, and different varieties There are large differences in the propagation conditions among different varieties of jujube trees, and the tissue culture methods of different varieties of jujube trees are also different.
[0005] At present, in the breeding of Moonlight jujube,

Method used

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  • Tissue culture rapid propagation method for moonlight jujubes

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Effect test

Embodiment 1

[0026] A method for tissue culture and rapid propagation of Moonlight Jujube, comprising the following steps:

[0027] (1) Establishment of explants: in mid-May, cut the 2-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 15 minutes, and then remove them at a mass concentration of Soak in 0.4% washing powder solution for 12 minutes, then rinse with tap water, then soak in 75% alcohol for 35 seconds, rinse with sterile water 5 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 10 seconds minutes, then rinsed with sterile water 7 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as explants;

[0028] (2) Start-up culture: Inoculate the sterilized explants into the high-temperature sterilized start-up medium for culture. The compos...

Embodiment 2

[0034] A method for tissue culture and rapid propagation of Moonlight Jujube, comprising the following steps:

[0035] (1) Establishment of explants: in mid-May, cut the 2-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 15 minutes, and then remove them at a mass concentration of Soak in 0.4% washing powder solution for 13 minutes, then rinse with tap water, then soak in alcohol with a mass concentration of 75% for 35 seconds, rinse with sterile water for 6 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 11 minutes, then rinsed with sterile water 8 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as explants;

[0036] (2) Start-up culture: Inoculate the sterilized explants into the high-temperature sterilized start-up mediu...

Embodiment 3

[0042] A method for tissue culture and rapid propagation of Moonlight Jujube, comprising the following steps:

[0043] (1) Establishment of explants: in mid-May, cut the 2-year-old jujube head branches, cut off the tender shoots and the aging parts of the base, and leave the semi-lignified stems, rinse with tap water for 15 minutes, and then remove them at a mass concentration of Soak in 0.4% washing powder solution for 15 minutes, then rinse with tap water, then soak in alcohol with a mass concentration of 75% for 35 seconds, rinse with sterile water 6 times, drain the water, and disinfect with 1.0% sodium hypochlorite solution for 12 minutes, then rinsed with sterile water 8 times, picked up the cleaned stems with tweezers, blotted the surface moisture with sterilized filter paper on the aseptic operating table, and cut 1.5-2.5cm budded stems as explants;

[0044] (2) Start-up culture: Inoculate the sterilized explants into the high-temperature sterilized start-up medium fo...

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Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a tissue culture rapid propagation method for moonlight jujubes. The method includes the steps of establishment of an explant, culture starting, subculture, rooting culture, hardening, transplanting and the like. The moonlight jujubes are studied, most suitable culture components are adopted at different stages, the propagation coefficient is high, the rooting rate is high, the method is easy and convenient to operate and low in cost, and a large number of moonlight jujube tissue culture seedlings can be acquired effectively and conveniently within a short time; the obtained tissue culture seedlings are high in survival rate, and the seedlings can keep good genetic characters of superior trees; a large number of orderly regenerated plants with stable genetic characters can be acquired through the tissue culture rapid propagation method for the moonlight jujubes, the test result is stable, repeatability is good, the method can be applied to large-scale commercial seedling growing, the industrial development of the moonlight jujubes is promoted, and the method is suitable for large-area regional planting in north mountainous areas of Guangxi, particularly planting in karst landform mountainous areas.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Moonlight jujube. Background technique [0002] Moonlight jujube is originally produced in Mancheng County, Hebei Province, and is called "Lingzao" locally. It is a new variety of extremely early maturity, high quality, high yield, cold resistance and fresh food selected by Hebei Agricultural University. Moonlight jujube is a rare variety of jujube tree evolved in long-term evolution. It has premature, high and stable yields, very small core, thin peel, fine and crisp flesh, rich juice, sweet and sour taste, can be eaten directly, and mature Early and other advantages, it is an excellent fresh variety that is widely promoted in current production. Moonlight jujube matures very early, and the fresh jujube has a long supply period; its fruit is medium-large, unique and beautiful in shape, with a single f...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 雷军强韦颖婷覃稳梅李玉秋莫晓君韦倩梅
Owner 象州县科学技术局
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