Tissue culture rapid propagation method for moonlight jujubes

Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a tissue culture rapid propagation method for moonlight jujubes. The method includes the steps of establishment of an explant, culture starting, subculture, rooting culture, hardening, transplanting and the like. The moonlight jujubes are studied, most suitable culture components are adopted at different stages, the propagation coefficient is high, the rooting rate is high, the method is easy and convenient to operate and low in cost, and a large number of moonlight jujube tissue culture seedlings can be acquired effectively and conveniently within a short time; the obtained tissue culture seedlings are high in survival rate, and the seedlings can keep good genetic characters of superior trees; a large number of orderly regenerated plants with stable genetic characters can be acquired through the tissue culture rapid propagation method for the moonlight jujubes, the test result is stable, repeatability is good, the method can be applied to large-scale commercial seedling growing, the industrial development of the moonlight jujubes is promoted, and the method is suitable for large-area regional planting in north mountainous areas of Guangxi, particularly planting in karst landform mountainous areas.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Moonlight jujube. Background technique [0002] Moonlight jujube is originally produced in Mancheng County, Hebei Province, and is called "Lingzao" locally. It is a new variety of extremely early maturity, high quality, high yield, cold resistance and fresh food selected by Hebei Agricultural University. Moonlight jujube is a rare variety of jujube tree evolved in long-term evolution. It has premature, high and stable yields, very small core, thin peel, fine and crisp flesh, rich juice, sweet and sour taste, can be eaten directly, and mature Early and other advantages, it is an excellent fresh variety that is widely promoted in current production. Moonlight jujube matures very early, and the fresh jujube has a long supply period; its fruit is medium-large, unique and beautiful in shape, with a single f...

AI Technical Summary

Problems solved by technology

So far, people have made some achievements in the study of jujube tissue culture, and the technology of using tissue culture to propagate fine varieties of jujube has been gradually applied to production. However, the tissue culture of jujube is difficult and expensive, and different varieties There are large differences in the propagation conditions among different varieties of jujube trees, and the tissue culture methods of different varieties of jujube trees are also different.

[0005] At present, in the breeding of Moonlight jujube, due to the use of traditional methods such as cuttings and grafting for breeding, there are problems such as very low breeding and reproduction rate, slow growth rate, and far from meeting the large demand of the market; The tissue culture and rapid propagation method of Moonlight Jujube can promote the rapid propagation of excellent genetic materials and realize the mass reproduction and rapid growth of Moonlight Jujube seedlings. It is an effective method to solve the market demand and maintain the characteristics of excellent trees.

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