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Method for purifying mercury and selenium in sewage through escherichia coli under aerobiotic and anaerobic conditions

A technology of Escherichia coli and anaerobic conditions, which is applied in the field of environmental protection and sewage purification treatment, can solve the problems of poisonous living organisms, and achieve the effects of no secondary pollution, low treatment cost and large treatment range

Inactive Publication Date: 2016-09-07
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

But the gap between harmful and beneficial doses of selenium is very small, and excess selenium is toxic to living organisms

Method used

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  • Method for purifying mercury and selenium in sewage through escherichia coli under aerobiotic and anaerobic conditions

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Embodiment

[0013] a, strain source and cultivation: the selected facultative anaerobic bacteria Escherichia coli, preservation number: GIM1.223, was purchased from Guangdong Microbial Strain Collection Center (GIM), and Escherichia coli was inoculated into components consisting of peptone 10‰, In the nutrient broth medium of beef extract 1.5‰, yeast extract powder 1.5g / L, and sodium chloride 5‰, cultivate it under aerobic and anaerobic conditions at a temperature of 20-30°C for 8-15 hours until the stable growth period. N 2 / H 2 As a gas to maintain anaerobic environment, the anaerobic environment is provided by DWSDG250 anaerobic workstation;

[0014] B, purification process: the Escherichia coli in the stable growth phase of step a is added to the water containing divalent mercury and selenite with an inoculum size of 3%-10% by volume, the initial concentration of divalent mercury and selenite in the water 40μg / L and 50mg / L, add 10-20g / L nutrient broth at the same time as the nutriti...

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Abstract

The invention relates to a method for purifying mercury and selenium in sewage through escherichia coli under the aerobiotic and anaerobic conditions. The method comprises the steps that enrichment culture is conducted on the escherichia coli in a nutrient broth culture medium under the aerobiotic and anaerobic conditions until the escherichia coli is in a stable growth period, then water containing divalent mercury and selenite radicals is inoculated with the escherichia coli, bacterium growth is maintained by taking nutrient broth as nutrition, after the escherichia coli is cultured for several days at room temperature under the aerobiotic and anaerobic conditions, the escherichia coli reduces selenite ions contained in the water into negative bivalent selenium ions, the negative bivalent selenium ions are combined with bivalent mercury ions to generate mercury selenide precipitates, the mercury selenide precipitates can stably exist in a water body without being absorbed and utilized by organisms, therefore, divalent mercury contamination and selenite radical contamination in the water body are simultaneously removed, harm to a human body is reduced, and secondary contamination is avoided. The method has the advantages of being low in cost, wide in application range and the like.

Description

technical field [0001] The invention relates to a method for purifying mercury and selenium in sewage under aerobic and anaerobic conditions by using Escherichia coli, and belongs to the technical field of sewage purification and treatment in the field of environmental protection. Background technique [0002] Mercury is the only heavy metal element that exists in liquid form under normal temperature and pressure conditions. The United States Environmental Protection Agency lists mercury as one of the 129 hazardous chemicals, and some people list mercury as one of the three most dangerous heavy metal elements, mainly because mercury is a non-essential element that has no beneficial effects on organisms Biological function, great harm to human body. Mercury entering the human body will mainly cause damage to the central nervous system, kidneys, liver, etc., and even endanger life safety. The reason why mercury can cause great harm to the human body is mainly because of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F3/30C02F101/20C02F101/10
CPCC02F3/30C02F3/34C02F2101/106C02F2101/20
Inventor 潘响亮王潇男宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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