Poly(p-phenylene vinylene) precursor, preparation method thereof and application of poly(p-phenylene vinylene) precursor to high-molecular fluorescent microspheres

Abstract

The invention discloses a poly(p-phenylene vinylene) precursor, a preparation method thereof and application of the poly(p-phenylene vinylene) precursor to high-molecular fluorescent microspheres. The preparation method includes taking hydroquinone as a starting material, substituting bromoacetic acid for hydrogen on a phenolic hydroxyl group to obtain p-phenoxy diacetate, introducing bromomethyl into an ortho-position of acetoxy on a benzene ring, substituting a thiophene group for bromine to obtain a polymer monomer, and preparing the poly(p-phenylene vinylene) precursor with carboxyls on a pendant group in an alkaline aqueous solution environment. The poly(p-phenylene vinylene) precursor is loaded on every matrix microsphere with amino or hydroxyl on the surface, and temperature is controlled during thermal elimination, so that the high-molecular fluorescent microspheres with different fluorescence emission wavelengths are obtained. The microspheres obtained from the poly(p-phenylene vinylene) precursor with a great number of carboxyls on the pendant group demonstrate the biological molecular reaction site characteristic from the surfaces, and accordingly the application field of the microspheres is expanded.

Description

technical field [0001] The invention relates to a precursor of poly(p-phenylenevinylene) with carboxyl groups in side groups and a preparation method thereof, which is applied to the preparation of polymer fluorescent microspheres with biomolecular reaction sites on the surface and adjustable fluorescence emission wavelengths The invention belongs to the technical field of fluorescent sensing materials. Background technique [0002] The poly(p-phenylenevinylene) precursor can be heat-treated at different temperatures to obtain poly(p-phenylenevinylene) with different fluorescence emission, and realize the adjustment of the fluorescence emission wavelength. The literature (Journal of Materials Chemistry, 1998, 8 (4), 919-924) reported a polyparaphenylene vinylene precursor that can be used to modify the substrate film, and the precursor is on the side group of each repeating unit With only one carboxyl group, poly(p-phenylenevinylene) can be loaded on the substrate through t...

AI Technical Summary

Problems solved by technology

The matrix microspheres used in CN 102660083 A have a particle size of 35 μm, and the prepared fluorescent microspheres have no reaction sites on the surface, and cannot be applied to fields such as biological detection

However, the matrix microspheres used in CN104371708 A have a particle size of 10 μm, and the layer-by-layer self-assembly technology is used to introduce carboxyl groups on the surface of the microspheres. However, the emission wavelength of the prepared fluorescent microspheres is single (about 500 nm), and the fluorescence emission of the microspheres cannot be achieved. The adjustment of wavelength cannot meet the needs of fluorescent coding technology, and the preparation method is cumbersome

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