SNP primers for early screening strongylocentrotus intermedius fine variety and screening method

A technology of intermediate balls and sea urchins, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as low output of progeny seedlings and poor genetic genes of parents

Active Publication Date: 2016-09-07
DALIAN OCEAN UNIV
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Problems solved by technology

In this way, during the growth process of sea urchins, it is necessary to measure the shell height, shell diameter and live weight of sea urchins regularly (such as every month, etc.), so as to carry out step-by-step screeni

Method used

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  • SNP primers for early screening strongylocentrotus intermedius fine variety and screening method
  • SNP primers for early screening strongylocentrotus intermedius fine variety and screening method
  • SNP primers for early screening strongylocentrotus intermedius fine variety and screening method

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] one. Screening process of SNP markers related to growth traits:

[0019] 1. Construction of High Density Genetic Linkage Map

[0020] 1.1 Mapping group construction

[0021] Using the pseudo-side crossing strategy, the second-generation male (♂) of Japanese self-propagating sea urchin and the female (♀) bred for more than five generations in Longwangtang, Lvshun, China were selected as the parents to cross-breed the F1 generation, and 194 F1 generations were constructed. Individuals and mapping populations of 2 parents were bred in the same environment.

[0022] 1.2 Enzyme digestion scheme determination

[0023] The sea urchin genome was selected as the reference genome sequence for electronic enzyme digestion prediction, and finally it was determined to use RsaI+HaeIII enzymes for enzyme digestion, and the length of the enzyme digestion fragment was 414-464bp.

[0024] 1.3 SLAF tag development and screening

[0025] Tissue DNA was extracted with a marine animal g...

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Abstract

The invention discloses SNP primers for early screening a strongylocentrotus intermedius fine variety and a screening method. The screening method comprises the following steps: selecting a strongylocentrotus intermedius second-generation male individual (Mars symbol) self-bred in Japan and a female individual (Venus symbol) bred for more than five generations in Longwangtang, Lvshun, China as male and female parents to crossbreed an F1 generation; establishing a mapping population containing 194 F1-generation individuals and two parents; cultivating in the same environment, extracting DNA, sequencing and taking other technical measures to find genetic markers of strongylocentrotus intermedius for the first time and design the relevant primers, wherein the primers can be used for early screening the strongylocentrotus intermedius fine variety. Compared with the prior art, the screening method has the advantages as follows: by the screening method, not only can manpower and material resources be saved and the seed selection be reduced, but also good genetic genes of the parents can be ensured, so that good progeny traits can be ensured.

Description

technical field [0001] The invention relates to a primer and a method for screening improved sea urchin intermedia, in particular to a SNP primer and a screening method for early screening improved sea urchin intermedius that can save manpower and material resources, reduce selection costs, and ensure good progeny traits. Background technique [0002] At present, traditional breeding methods such as crossbreeding and selective breeding are mostly used for the breeding of sea urchins intermedia. No matter it is crossbreeding or selective breeding, the parents are selected based on sea urchins with good growth characteristics. In this way, during the growth process of sea urchins, it is necessary to measure the shell height, shell diameter and live weight of sea urchins on a regular basis (such as every month, etc.), so as to carry out step-by-step screening, which not only consumes a lot of manpower and material resources, but also has all kinds of problems. The problem that ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 常亚青李丹丁君张伟杰
Owner DALIAN OCEAN UNIV
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