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Pesticide residue detection method and detection kit

A pesticide residue detection and kit technology, which is applied in chemical analysis by titration, material analysis by observing the impact on chemical indicators, instruments, etc., can solve the problems of unrecyclable, expensive tool enzymes, and high detection costs , to achieve the effect of easy observation, easy operation and high sensitivity

Inactive Publication Date: 2016-09-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing on-site detection methods for pesticide residues have shortcomings, mainly because the tool enzymes used in the existing methods are expensive and cannot be recycled after use. In addition, no matter free or immobilized tool enzymes need to be stored and transported at low temperature, resulting in high detection costs

Method used

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  • Pesticide residue detection method and detection kit
  • Pesticide residue detection method and detection kit
  • Pesticide residue detection method and detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0064] This embodiment provides a method for making pesticide residue detection test paper, which specifically includes the following steps:

[0065] S1. Weigh 30 grams of silk, and the molar concentration is 0.02 Na 2 CO 3 Boil in an aqueous solution for 30 minutes, wash with deionized water, place in a fume hood to air-dry, place in lithium bromide with a molar concentration of 9.3, dissolve at 60 degrees for 4 hours to become a silk fibroin solution. Put the silk fibroin solution into a dialysis bag, dialyze in deionized water for 36 hours, change the water 6 times during the period; centrifuge to remove impurities, and dilute the obtained silk fibroin aqueous solution to a concentration of 4%;

[0066] S2. Take 2ml of silk fibroin solution and place it in an ultrasonic cell pulverizer. The ultrasonic power is 20-40%, and the ultrasonic time is 1-10 minutes. The ultrasonic power is preferably 30%, and the time is preferably 3-4 minutes; add acetylcholine therein Esterase,...

Embodiment 2

[0069] This embodiment adopts the test paper-enzyme-silk fibroin film provided in the first embodiment, and provides a method for detecting pesticide residues, which specifically includes the following steps:

[0070] S1. Take 50 μL of different concentrations of pesticides and 900 μL of disodium hydrogen phosphate-potassium dihydrogen phosphate buffer pH=8 with a concentration of 0.1mol / L and pH=8.0, and add them to the test paper-enzyme-silk fibroin In the protein-filmed 24-well plate, add 50 μL of paraoxon pesticides with concentrations of 0, 0.002, 0.02, 0.2, 1, 2, 4, 10, 20, 1000, and 200 mg / L into different wells, at 37°C Incubate for 15 minutes

[0071] S2. After the membrane is completely wetted, add 50 μL of acetylcholine solution with a concentration of 0.2 mol / L to each well, and react at 37°C. After 30 minutes, check the color of the test paper. figure 1 As shown, the concentration of pesticides added in the figure is 0, 0.002, 0.02, 0.2, 1, 2, 4, 10, 20, 1000, 20...

Embodiment 3

[0073] This embodiment adopts the test paper-enzyme-silk fibroin film provided in the first embodiment, and provides a method for detecting pesticide residues in fruits and vegetables, including the following steps:

[0074] S1. Grind the vegetables to be tested stained with pesticides, take a certain amount and add them to the centrifuge tubes respectively, and then add a certain amount of phosphate buffer solution with a concentration of 0.01-0.1mol / L to the centrifuge tubes. Shake for 10 minutes;

[0075] S2. Take out the mixed solution, add it to the prepared test paper-enzyme-silk fibroin film, and incubate at 37°C for 15 minutes;

[0076] S3. After the membrane is completely wetted, add acetylcholine solution, react at 37°C, and compare the color with the standard color card after 10 to 30 minutes.

[0077] The present invention takes cabbage contaminated with pesticides of different specific concentrations as an example to describe in detail, and the specific steps are...

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Abstract

The invention relates to the field of quick detection of food safety, in particular to a pesticide residue detection method and a detection kit. The method comprises the following steps: S1, embedding: embedding silk fibroin and acetylcholin esterase into a multi-hole substrate to repaper an enzyme substrate; S2, sample preparation: uniformly mixing a sample to be detected with a buffer solution to obtain a sample solution; S3, detection: adding the sample solution to the enzyme substrate for incubation, then adding an acetyl cholinesterase substrate for incubation for a period of time, and detecting the mixture to obtain a pesticide residue result in the sample to be detected by adopting a pH method, a potentiometric titration method, a manometric method or a colorimetric method. The kit comprises the multi-hole substrate embedded with the silk fibroin and the acetylcholin esterase, the buffer solution and an acetyl cholinesterase substrate solution. The pesticide residue detection method and the detection kit are used for detecting organophosphorus and carbamate insecticides, and are low in detection cost, high in stability and high in sensitivity.

Description

technical field [0001] The invention relates to the field of rapid detection of food safety, in particular to a pesticide residue detection method and a detection kit. Background technique [0002] my country is a big country in the production and use of pesticides, and the problem of pesticide residues in agricultural products is very serious. This not only affects my country's food export trade, but also poses a huge threat to human health and the ecological environment. Among them, organophosphorus (OPs) and carbamate (CBs) insecticides have been screened out as specific inhibitors of acetylcholinesterase in the insect nervous system and are widely used, accounting for more than 70% of the insecticide usage. In view of the severe toxicity of pesticides and their great threat to the environment and human health, it is particularly important to develop high-sensitivity and high-accuracy detection methods for pesticide residues. [0003] Traditional pesticide residue detect...

Claims

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Application Information

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IPC IPC(8): G01N21/80G01N31/16G01N21/78G01N7/18
CPCG01N21/80G01N7/18G01N21/78G01N31/16
Inventor 郑兆柱王晓沁鲁镝思
Owner SUZHOU UNIV
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