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Biological method for preparing nano-silver

A technology of nano-silver and nano-silver particles, which is applied in the biological field of preparing nano-silver, can solve the problems of product stability, toxic reagent residues, disputes, hazards, etc. Effect

Inactive Publication Date: 2016-09-14
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physical or chemical reduction methods are well applied because of the advantages of simple experimental conditions, low cost, and energy saving. Currently, these methods are mostly used in commercial scale production, but the product stability and toxic reagents involved are The problem of residues is very controversial, because the chemical reagents used are harmful to the human body and the environment to a certain extent, and stabilizers need to be added to prevent the aggregation of nanoparticles, and most stabilizers or dispersants are carcinogenic, thus limiting Application of AgNPs in medicine, food and other fields

Method used

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  • Biological method for preparing nano-silver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Cultivation of fungi.

[0031] Galactomycesgeotrichum KG-1 strain, stored on PDA slant at 4°C. Use an inoculation needle to inoculate mycelium into YPD culture medium, cultivate at 120rpm and 25°C for 5 days to obtain an activated fermentation broth; take the activated fermentation broth, and culture it in a new YPD culture medium at 120rpm and 25°C for 4 days according to 10% inoculum .

[0032] YPD formula is: yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000mL, sterilized at 121°C for 20min.

Embodiment 2

[0033] Embodiment 2: bacterial cell separation

[0034] Get the lactic acid bacteria fungal culture obtained in Example 1, filter with 4 layers of medical gauze, discard the filtrate, and keep the thalline. The cells were further washed with deionized water until the filtrate was colorless to obtain wet cells.

Embodiment 3

[0035] Embodiment 3: the preparation of thalline soaking liquid

[0036] Take 100 g of the fungal thallus obtained in Example 2, add 500 ml of purified water, and place it under room temperature and natural light for 48 hours. Then filter with 4 layers of gauze or filter paper, and collect the filtered clear liquid, which is the cell soaking liquid.

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Abstract

The invention discloses a biological method for preparing nano-silver. Independently separated and screened fungi strain Galactomycesgeotrichum KG-1 is adopted to be cultivated, products secreted by thallus of the fungi strain Galactomycesgeotrichum KG-1 in the innutritious pure water environment are utilized, silver nitrate is restored to obtain nano-silver particles, and through simple centrifugal treatment, nano-silver particle precipitation is obtained; and the method is easy to operate, mild in condition and capable of meeting the green and ecological development tendency.

Description

technical field [0001] The invention belongs to the field of food preservation materials, and in particular relates to a biological method for preparing nano silver. Background technique [0002] In recent years, there have been many studies on the synthesis of metal nanoparticles. Nanoparticles refer to particles with a size between 1 and 100nm. They are located in the transition zone between atomic clusters and macroscopic objects. They have unique physical and chemical properties and photoelectric properties. They are widely used in electronics, optics, chemical ceramics, biology and medicine. Has been widely used. Humans have long recognized that silver has a broad-spectrum bactericidal effect and is used for sterilization and disinfection. Therefore, among many nanomaterials, nano-silver (AgNPs) has the most extensive and in-depth research, and reports on the antibacterial activity of nano-silver emerge in endlessly. Nano-silver also has anti-virus, anti-tumor and oth...

Claims

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Application Information

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IPC IPC(8): B22F9/24C12N1/14B82Y40/00C12R1/645
CPCB22F9/24B82Y40/00C12N1/14
Inventor 刘小莉周剑忠刘源夏秀东李莹王英张丽霞黄自苏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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