Method for producing probiotic functional food through buckwheat fermentation
A technology of fermented food and probiotics, applied in food science and other directions, can solve the problem of few types of products beneficial to diabetics, and achieve the effect of significantly lowering blood sugar, blood lipid and blood pressure, low cost and easy production.
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[0052] Embodiment 1. Probiotic liquid fermented beverage technology-determination of buckwheat and grain ratio
[0053] (1) After the buckwheat is hulled, the buckwheat and grains are washed, and the powder is passed through a 100-mesh sieve;
[0054] (2) Preparation of activation medium: add 5g of buckwheat flour per 100ml of water (the type of buckwheat flour in the activation medium is the same as the type of buckwheat flour in the fermentation medium); sterilize the activation medium under the conditions of 121°C, 20min ;
[0055] (3) Preparation of fermentation medium: add 5g sweet buckwheat flour / tartary buckwheat flour, 0-5g soybean flour / oat flour / wheat flour / corn flour / black bean flour per 100ml of water, sterilize at 121°C for 20min;
[0056] (4) Activation of strains, Lactobacillus plantarum and Bifidobacterium animalis glycerol tubes were respectively inoculated into the activation medium at 1% for activation, pH 7.2-7.4, and the control temperature was 37°C; afte...
Example Embodiment
[0090] Example 2: Probiotic liquid fermented beverage technology-determination of probiotic inoculum amount
[0091]Other conditions are the same as in Example 1, according to the ratio of sweet buckwheat flour: soybean flour is 5g: 2g configuration fermentation medium, Lactobacillus plantarum is inoculated according to the following amount of 1 × 10 6 , 5×10 6 , 1×10 7 , 5×10 7 CFU / mL were respectively inoculated into the fermentation medium, simultaneously inoculated with Bifidobacterium animalis, and the inoculation ratio of Lactobacillus plantarum and Bifidobacterium animalis was 1:1, and the fermentation was carried out. After the fermentation was completed, the viable probiotic bacteria in the fermentation broth were determined number.
[0092] The results are shown in Table 11: the optimal inoculum amounts of Lactobacillus plantarum TK9 and Bifidobacterium animalis 937 are both 5×10 7 CFU / mL. The total number of viable bacteria measured was 3.5 × 10 9 CFU / mL, of w...
Example Embodiment
[0100] Example 3: Probiotic liquid fermented beverage technology-determination of culture time
[0101] (1) Under the condition that the inoculation amount has been optimized, the liquid fermentation medium is selected to add 5g of sweet buckwheat flour and 2g of soybean flour per 100ml of water.
[0102] Lactobacillus plantarum TK9 and Bifidobacterium animalis 937 glycerol tubes were inoculated into sweet buckwheat activation medium at 1% for primary activation, both were activated for 20 hours, and then transferred to secondary activation culture at 10% (v / v) inoculum In the base, the secondary activation is 24h. The liquid fermentation medium was simultaneously inoculated with Lactobacillus plantarum TK9 and Bifidobacterium animalis 937 seed liquor for mixed bacterial fermentation.
[0103] Lactobacillus plantarum TK9 and Bifidobacterium animalis 937 were inserted into the liquid fermentation medium of sweet buckwheat, both of which were 5×10 7 CFU / mL; pH 7.2-7.4, culture...
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