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Determination method for linarin in Buddleja officinalis

A determination method and a technology for mongolic acid, applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills, complex pharmacopoeia operation process, etc., and achieve the effects of good repeatability, consistent accuracy and simple operation.

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

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  • Determination method for linarin in Buddleja officinalis
  • Determination method for linarin in Buddleja officinalis
  • Determination method for linarin in Buddleja officinalis

Examples

Experimental program
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Embodiment 1

[0037] Pulverize the Mimeng flower through a pulverizer, pass through a No. 3 sieve, weigh about 0.5g, and mix it with 0.5g of diatomaceous earth. Add an appropriate amount of diatomaceous earth to the extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, transfer the extract to a 50ml volumetric flask, dilute to the mark with methanol, centrifuge at 15000r / min for 5min, take the supernatant, and enter it into LC for determination.

[0038] The extraction conditions (see table 1) are:

[0039] Table 1

[0040]

[0041] Analysis method is LC liquid chromatography, liquid chromatography analysis conditions:

[0042] a) Instrument: Thermo double ternary liquid phase (U-3000);

[0043] b) Chromatographic column: Thermo Syncronis C18 3*100mm 3um, that is, a carbon-18 chromatographic column with a diameter of 3mm, a length of 100mm, and a particle size of 3 microns;

[0044] c) ...

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PUM

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Abstract

The invention discloses a determination method for linarin in Buddleja officinalis. The method comprises the following steps: step 1, crushing Buddleja officinalis and then extracting the crushed Buddleja officinalis by using an ASE extraction method so as to obtain extract; step 2, subjecting the extract obtained in the previous step to centrifugation so as to obtain supernatant; and step 3, analyzing the supernatant by using liquid chromatography. Analysis conditions of liquid chromatography are that a) a used instrument is a dual three-component liquid chromatograph; b) a C-18 chromatographic column with specifications of 3*100 mm and 3 [mu]m is employed as a chromatographic column; c) column temperature is 40 DEG C; d) flow velocity is 0.5 mL / min; e) a mobile phase is a methanol-0.1% acetic acid solution, wherein a volume ratio of methanol to an acetic acid solution with a mass percent of 0.1% at 20 DEG C is 48: 52; and f) detection wavelength is 326 nm. The determination method for linarin in Buddleja officinalis is simple to operate and high in detection precision.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for determining saffron glucosinolate in Budisma japonica. Background technique [0002] In 2015, the Pharmacopoeia recorded the method of extracting fenugreek from Budisma japonica: Take about 0.5g of the coarse powder of this product, weigh it accurately, put it in a Soxhlet extractor, add 100ml of petroleum ether (60-90°C), and heat to reflux for 2 hours, discard the petroleum ether, evaporate the dregs, add 100ml of methanol and continue to heat and reflux for 4 hours, put the extract in an evaporating dish, concentrate to an appropriate amount, and transfer it to a 50ml measuring bottle. Incorporate into the same measuring bottle. Add methanol to the mark, shake well, filter, and take the continued filtrate, that is, too. Centrifuge at 15000r / min for 3min, take the supernatant, and enter the LC assay. [0003] The parameters of its liquid chromatography are: octa...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 陈学松王丽丽韦涛梁美艳黄新惠
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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