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A method for efficiently degrading sinapine in rapeseed meal based on germinated rapeseed enzymatic method

A technology of sinapine and rapeseed, which is applied in food processing, animal husbandry, and additional food elements, etc., can solve the problems of high-efficiency enzyme production and degradation of rapeseed sinapine, etc., and achieves easy realization, small damage, and simple process Effect

Active Publication Date: 2019-10-18
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no literatures and patents on germinating rapeseed to make it efficiently produce enzymes and degrade rapeseed sinapine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] A method for efficiently degrading sinapine in rapeseed meal by enzymatic method based on germinated rapeseed comprises the following steps:

[0031] (1) Rapeseed germination: take 5 germination boxes, first spread 4 layers of toilet paper on the bottom, and then spread a layer of emery cloth on top, sprinkle water to moisten. Weigh 3g rapeseed, disperse evenly on the emery cloth, sprinkle water to make the rapeseed appear to be in a wet state. Set the temperature of the constant temperature and humidity box to 30°C, put the germination box into the constant temperature and humidity box, observe whether the rapeseed is in a humid state every day, and add water appropriately according to the situation. After 4 days of germination, the rapeseed sprouts were removed and placed in a -20°C refrigerator to terminate germination.

[0032] (2) Crude enzyme liquid extraction: pulverize the rapeseed sprouts, weigh 10g and place it in a conical flask, add 50mL of sodium phosphate...

example 2

[0038] A method for efficiently degrading sinapine in rapeseed meal by enzymatic method based on germinated rapeseed comprises the following steps:

[0039] (1) Germination of rapeseed: with embodiment 1.

[0040] (2) Crude enzyme liquid extraction: pulverize the rapeseed sprouts, weigh 10g and place it in a conical flask, add 80mL of sodium phosphate buffer solution with a concentration of 8mM and a pH value of 6.5, mix well and place it on a magnetic stirrer, set Rotate at 300rmp, extract for 25min. Afterwards, the supernatant, namely the crude enzyme solution, was obtained by centrifugation under the condition of rotating speed of 15000 rpm.

[0041] (3) Enzymolysis: Weigh 50 g of rapeseed meal into an Erlenmeyer flask, add 150 mL of sodium phosphate buffer solution with a concentration of 10 mM and a pH value of 6.5 and 50 mL of crude enzyme solution, and stir well to make the system evenly mixed. Place the Erlenmeyer flask on a water bath shaker, set the temperature at ...

example 3

[0046] A method for efficiently degrading sinapine in rapeseed meal by enzymatic method based on germinated rapeseed comprises the following steps:

[0047] (1) Germination of rapeseed: with embodiment 1. Only the germination temperature is 28°C, and the germination time is 5 days.

[0048] (2) Crude enzyme liquid extraction: pulverize the rapeseed sprouts, weigh 5g and place it in a conical flask, add 20mL of sodium phosphate buffer solution with a concentration of 15mM and a pH value of 7.5, mix well and place it on a magnetic stirrer, set Rotate at 300rmp, extract for 20min. Then centrifuge under the condition of rotating speed of 10000rmp to obtain the supernatant, that is, the crude enzyme solution.

[0049] (3) Enzymatic hydrolysis: Weigh 10 g of rapeseed meal into an Erlenmeyer flask, add 30 mL of sodium phosphate buffer solution with a concentration of 12 mM and a pH value of 7.5 and 8 mL of crude enzyme solution, and stir well to make the system evenly mixed. Place...

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PUM

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Abstract

The invention relates to a method for efficiently degrading sinapine in rapeseed meal based on a germinating rapeseed enzymic method. The method comprises the following steps that firstly, rapeseeds germinate, wherein the rapeseeds with germination vigor germinate in a constant-temperature and constant-humidity chamber, and water is added according to needs in the germination period to enable the rapeseeds to be in a wet state all the time; secondly, crude enzyme is extracted, wherein germination of the rapeseeds is terminated, then, the rapeseeds are smashed, sodium phosphate buffer solution is used for stirring extraction, and after centrifugal separation, liquid supernatant is taken and serves as the crude enzyme; thirdly, enzymolysis is carried out, wherein the sodium phosphate buffer solution is added into the rapeseed meal, then, the crude enzyme is added, and sufficient vibration is carried out for enzymatic reaction. The method is used for degradation of the sinapine in the rapeseed meal, and has the advantages of being high in degradation efficiency, high in specificity and environmentally friendly (no organic solvent is involved in the whole process) and not damaging the nutritional quality of the rapeseed meal.

Description

technical field [0001] The invention relates to the technical field of food chemistry and comprehensive utilization of rapeseed, in particular to a method for degrading sinapine in rapeseed with enzymes produced by germinated rapeseed. Background technique [0002] Rapeseed is rich in polyphenols, and the defatted rapeseed meal (referred to as rapeseed meal) contains about 3%, which is about 30 times that of soybean meal. Rapeseed polyphenols are divided into phenolic acids and tannins, and phenolic acids include free phenolic acids and esterified phenolic acids, of which free phenolic acids account for 9-16% of the total phenols, and sinapic acid is the most important free phenolic acid. Acid: Sinapine is the choline ester of sinapinic acid, the most important esterified phenolic acid in rapeseed, accounting for about 80% of the total phenols, and the content in rapeseed is about 0.4-1.0%. After pressing and leaching to make oil, most of the polyphenols remain in rapeseed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/37A23K20/189
CPCY02P60/87
Inventor 郑畅杨湄周琦黄凤洪李文林张苗刘昌盛万楚筠
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI