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Antibacterial combination containing nano silver and kanamycin

A kanamycin and nano-silver technology, applied in antibacterial drugs, medical preparations containing active ingredients, and resistance to vector-borne diseases, etc., can solve problems such as increased treatment costs, difficulties in antibacterial drugs, and threats to human health

Inactive Publication Date: 2016-10-12
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the widespread use of antibacterial drugs, bacterial resistance soon appeared, and bacteria like "super bacteria" that were difficult to kill by current antibacterial drugs appeared, which seriously threatened human health and increased treatment costs. social burden
However, the development of new antibacterial drugs is currently difficult and slowed down

Method used

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  • Antibacterial combination containing nano silver and kanamycin
  • Antibacterial combination containing nano silver and kanamycin
  • Antibacterial combination containing nano silver and kanamycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The separate or combined drug susceptibility analysis of embodiment 1 is carried out by micro broth dilution method and checkerboard dilution method, and the specific methods are as follows:

[0032] (1) Micro broth dilution method

[0033] Take out 200 μL of the monoclonal bacterial solution from the -80 °C refrigerator and add it to 5 mL of MH broth medium, and place it in a constant temperature shaker at 37 °C and 150 rpm for about 4 h. The bacterial solution changes from clarification to turbidity. reached the logarithmic growth phase. Use MH broth medium to adjust the concentration of the bacterial solution to make it OD 600 The value falls around 0.1, at this time the concentration of the bacterial solution is about 10 8 CFU / mL. Dilute the above bacterial solution to 2×10 5 CFU / mL was defined as the concentration of the loaded bacterial solution. The antimicrobial drugs to be tested (according to the experimental concentration of each drug) were diluted 10 t...

Embodiment 2

[0044] Example 2 further explores the mechanism of synergistic effect by TEM, NTA and ultraviolet.

[0045] The specific mode of action between the bacteria and the combined system was further observed by TEM. The final concentration of the bacterial solution was 10 7 CFU / mL Escherichia coli ATCC 25922 was treated with a certain concentration of nano-silver alone or in combination with kanamycin for 12 hours, and then the bacteria were collected by centrifugation, fixed with 2.5% glutaraldehyde for 2 hours, dehydrated step by step, and the final dispersion was dropped on copper online, and dried to prepare bacterial samples.

[0046] Figure 4 It can be clearly seen that when nano-silver alone acts on bacteria, there is no obvious adhesion of nano-silver on the surface of bacteria. When combined with kanamycin, a large amount of nanosilver adhered to the bacterial surface was clearly seen, which would lead to an increase in the local silver concentration on the bacterial s...

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Abstract

The invention provides a highly efficient antibacterial combination of nano silver (including nano silver, polyvinylpyrrolidone-modified nano silver and sodium citrate-modified nano silver) and kanamycin. Whether sensitive strains (escherichia coli) or drug-resistant strains (pseudomonas aeruginosa), compared with single drug administration, combined effects of a low dose of the nano silver and a low dose of the kanamycin can achieve synergistic antibacterial effect, that is to say, when the two components are combined, the concentrations of the two components are both less than 1 / 4 of the concentration of the nano silver or the kanamycin during alone acting, and a significant synergistic antibacterial effect is generated. The combined synergistic antibacterial effect of the sodium citrate-modified nano silver and the kanamycin is most significant. The combination method is simple, the cost is low, and the bactericidal effect is excellent; the antibacterial combination has good bactericidal property on the drug-resistant strains, can be applied in combined use of antibacterial agents in the medicines and agricultural production fields, and provides basic data and use guidance.

Description

Technical field [0001] The present invention provides nano -silver of different surface groups and common antibacterous antibiotics when combined with medication.Low-dose nano-silver (AG NPS), polyethylene pyrirol-modified nano-silver (AG-PVP NPS) and sodium citrate modified nano-silver (AG-CIT NPS) and low-dose carnamycin jointly act on threatening the human bodyHealthy common bacteria (E. coli and Greenworm) have obvious antibacterial effects.It is used in the field of medical antibacterial technology and agricultural production. Background technique [0002] Antibacterial drugs are used to treat various bacterial infections or their pathogenic microorganisms.Its discovery and application are one of the most important contributions to human health and become an important foundation for the success of modern medicine.Not only human beings, but also agricultural production cannot be separated. It plays an important role in preventing germs from infection and promoting animal grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/38A61K31/7036A61P31/04
CPCA61K31/7036A61K33/38A61K2300/00Y02A50/30
Inventor 王海芳武迪杨毅凡陈妮汪坤曹傲能
Owner SHANGHAI UNIV
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