A method for extracting high-purity ceramide from rice bran

A ceramide, high-purity technology, applied in the separation/purification of carboxylic acid amide, organic chemistry, etc., can solve the problems of increasing the difficulty of purification treatment, more impurities in the extract, and increasing the purification process, so as to shorten the extraction cycle and efficiency, and extract The effect of shortening the time and saving the extraction solvent

Active Publication Date: 2018-09-04
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many impurities in the extract, which increases the difficulty of subsequent purification treatment, increases the purification process, and prolongs the production cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Pre-treatment: remove impurities from the dried rice bran raw material, pulverize, and sieve to obtain 20-40 mesh rice bran raw material powder, then take 500kg rice bran raw material powder and add sphingomyelinase preparation enzymatic solution (3kg sphingomyelinase preparation is dissolved in 1500L of pure water), at 36°C for 70 minutes, and filtered to obtain 1849 kg of enzymatically hydrolyzed rice bran (containing water). After the enzymolyzed rice bran raw material was dried, the content of the target product ceramide was 0.076%;

[0042] (2) Microwave countercurrent extraction: Add 2550L of ethanol (88% volume fraction) to 1849kg of enzymatically hydrolyzed rice bran (containing water) obtained in step (1), and conduct microwave countercurrent at 55°C with a microwave power of 700W and a microwave frequency of 7000MHz Extract for 50 minutes, heat-preserve and filter to obtain 2515 kg of rice bran extract;

[0043] (3) Concentration: In a vacuum concentrator,...

Embodiment 2

[0050] (1) Pre-treatment: Remove impurities from the dried rice bran raw material, crush and sieve to obtain 20-40 mesh rice bran raw material powder, then take 500kg rice bran raw material powder and add sphingomyelinase preparation enzymatic solution (4kg sphingomyelinase preparation is dissolved in 1500L of pure water), at 38°C for 90 minutes, filtered to obtain 1856 kg of enzymatically hydrolyzed rice bran (containing water), and the content of the target product ceramide after drying the enzymatically hydrolyzed rice bran raw material was 0.077%;

[0051] (2) Microwave countercurrent extraction: add 3000L of ethanol (86% volume fraction) to 1856 kg of enzymatically hydrolyzed rice bran (containing water) obtained in step (1), and conduct microwave extraction at 60°C with a microwave power of 900W and a microwave frequency of 5000MHz. Countercurrent extraction for 45 minutes, insulation and filtration, to obtain 2985 kg of rice bran extract;

[0052] (3) Concentration: In ...

Embodiment 3

[0059] (1) Pre-treatment: Remove impurities from the dried rice bran raw material, crush and sieve to obtain 20-40 mesh rice bran raw material powder, then take 500kg rice bran raw material powder and add sphingomyelinase preparation enzymatic solution (5kg sphingomyelinase preparation is dissolved in 1500L of pure water), at 37°C for 90 minutes, and filtered to obtain 1838 kg of enzymatically hydrolyzed rice bran (containing water). After the enzymolyzed rice bran raw material was dried, the content of the target product ceramide was 0.0775%;

[0060] (2) Microwave countercurrent extraction: Add 3200L ethanol (88% volume fraction) to 1838 kg of enzymatically hydrolyzed rice bran (containing water) obtained in step (1), and conduct microwave extraction at 60°C with a microwave power of 900W and a microwave frequency of 5000MHz. Countercurrent extraction for 50 minutes, heat preservation and filtration, to obtain 3195 kg of rice bran extract;

[0061] (3) Concentration: In a va...

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Abstract

The invention discloses a method for extracting high-purity ceramide from rice bran. The method comprises the following steps: (1) pretreatment: cleaning the rice bran raw material, grinding and sieving; and then performing enzymolysis and filtration to obtain enzymolysis rice bran; (2) microwave countercurrent extraction: adding an organic solvent into the enzymolysis rice bran, and performing microwave countercurrent extraction and thermal-insulation filtration to obtain rice bran extract; (3) concentration: concentrating the rice bran extract and recycling the organic solvent to obtain a rice bran concentrate; (4) organic solvent extraction and separation: stirring and extracting the rice bran concentrate with the organic solvent, and performing vacuum concentration to obtain a tarry lipid mixture; (5) performing silica gel chromatography adsorption separation, eluting the organic solvent and collecting the ceramide target fraction; and (6) concentrating and drying to obtain a ceramide product. The method disclosed by the invention has the advantages of simple technology and low energy consumption and cost and is suitable for industrial continuous production; and the purity of the obtained ceramide product is greater than or equal to 99%, and the yield is greater than or equal to 0.075%.

Description

technical field [0001] The invention relates to a method for extracting high-purity substances from rice bran, in particular to a method for extracting high-purity ceramide from rice bran which is suitable for industrial production. Background technique [0002] Rice bran is the main by-product of rice processing. Research results at home and abroad show that rice bran is rich in various nutrients and physiologically active substances. In Japan, rice bran oil was used as cosmetics as early as the 1950s and 1960s. Formal research on the ingredients in rice bran oil began in 1956. Especially in recent studies, it was found that rice bran contains ceramide (Eeramide) and squalene (Squalene), which are very compatible with the skin. Therefore, there are Patent literature proposes that rice bran can be used to directly produce cosmetic facial masks. [0003] At present, there are many studies on the nutritional function of rice bran and its application in livestock and poultry...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C235/08C07C233/18C07C231/24
CPCC07C231/24C07C235/08C07C233/18
Inventor 龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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