Culture medium for producing kasugamycin by fermenting actinomyces microaureus and culture method

Abstract

The invention relates to a culture medium for producing kasugamycin by fermenting actinomyces microaureus and a culture method. According to the culture medium and the culture method, disclosed by the invention, a seed culture medium for producing the kasugamycin by fermenting the actinomyces microaureus, a carbon source and a nitrogen source in a fermentation culture medium and the optimal matching proportion are confirmed; particularly, oil sludge is added in the culture medium, so that the using amount of carbon source cottonseed oil is reduced; fermented dried bean curd dregs are added in the fermentation culture medium, so that the using amount of chrysalis oil is reduced. By utilizing a culture medium formula and a fermentation control technology which are provided in the invention, the fermentation unit of the kasugamycin can be increased, the fermentation cost can be reduced, the influence of raw auxiliary materials on the fermentation unit can be reduced to the maximum, the sources of the raw auxiliary materials are not influenced by the environment, sufficient supply of the kasugamycin is ensured, and stable and efficient production of the kasugamycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a culture medium and a culture method for fermenting and producing kasugamycin by Actinomyces aureus. Background technique [0002] Kasugamycin is a weakly alkaline antibiotic produced by Streptomyces. It is named Little Aureus Streptomycin because the producing bacteria secrete aureus in the culture medium. Kasugamycin is a dual-use antibiotic for agriculture and medicine. [0003] At present, domestic fermentation production of kasugamycin adopts a three-stage fermentation mode. The main problems of this method are as follows: [0004] 1 The fermentation level of industrialized large-scale production is low, and its fermentation level is maintained at about 10000-12000u / L. [0005] 2 There is a lack of literature reports on the complete fermentation process of kasugamycin in China, and the relevant literature reports are mainly experiments, which cannot provide effectiv...

AI Technical Summary

Problems solved by technology

[0005] 2 There is a lack of literature reports on the complete fermentation process of kasugamycin in China, and the relevant literature reports are mainly experiments, which cannot provide effective technical support for large-scale production models

[0006] 3 The large-scale production of kasugamycin has a long fermentation cycle, generally around 170 hours, and high energy consumption

[0007] 4 The production cost of Kasugamycin fermentation is relatively high, and the product lacks market competitiveness