Culture medium for producing kasugamycin by fermenting actinomyces microaureus and culture method

A technology of actinomycetes aureus and fermentation medium, applied in the field of fermentation, can solve the problems of high energy consumption, long kasugamycin fermentation cycle, and lack of literature reports on kasugamycin fermentation technology, so as to increase the fermentation unit and reduce the fermentation rate. The effect of energy consumption

Inactive Publication Date: 2016-10-12
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 There is a lack of literature reports on the complete fermentation process of kasugamycin in China, and the relevant literature reports are mainly experiments, which cannot provide effective technical support for large-scale production models...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Primary seed culture: primary seed medium volume 1m 3 .

[0068] Primary seed medium:

[0069] Cottonseed oil 4L, sludge 3kg, starch 7kg, cottonseed cake powder 11kg, silkworm chrysalis oil 4L, corn steep liquor 11L, ammonium sulfate 0.4 kg, light calcium carbonate 1kg.

[0070] Primary seed culture: Firstly, sterilize the primary seed medium and cool it to 20°C, and keep the pressure with sterile air. The quality of the primary seed medium after sterilization: amino nitrogen 30mg / L, dissolved phosphorus 120ug / ml, Fat 6g / L. Then, under the protection of the flame, the cultured Actinomyces aureus mother bottle fermentation liquid was mixed at 2 / m 3 The inoculum amount of the inoculum is transferred to the primary seed medium for primary seed cultivation. The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 20m 3 / h; the stirring speed is 60r / min; the bacterial cell concentration is 28%, and the culture time is 2...

Embodiment 2

[0092] Primary seed culture: primary seed medium volume 1m 3 .

[0093] Primary seed medium:

[0094] Cottonseed oil 5L, oil sludge 4kg, starch 8kg, cottonseed cake powder 12kg, silkworm chrysalis oil 5L, corn steep liquor 12L, ammonium sulfate 0.5kg, light calcium carbonate 2kg.

[0095] Primary seed cultivation: first sterilize the primary seed medium and cool it to 21°C, and keep the pressure with sterile air. The quality of the primary seed medium after sterilization: amino nitrogen 32mg / L, dissolved phosphorus 126ug / ml, Fat 6.5g / L. Then, under the protection of the flame, the fermented liquid of the mother bottle of Actinomyces aureus that has been cultivated is mixed at a rate of 2.2 / m 3 The amount of inoculum was transferred to the primary seed medium for seed cultivation. The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 25m 3 / h; the stirring speed is 65r / min; the bacterial cell concentration is 29%, and t...

Embodiment 3

[0117] Primary seed culture: primary seed medium volume 1m 3 .

[0118] Primary seed medium:

[0119] Cottonseed oil 6L, oil sludge 5kg, starch 9kg, cottonseed cake powder 13kg, silkworm chrysalis oil 6L, corn steep liquor 13L, ammonium sulfate 0.6kg, light calcium carbonate 3kg.

[0120] Primary seed culture: first sterilize the primary seed medium and cool it to 22°C, and keep the pressure with sterile air. The mass of the primary seed medium after sterilization: amino nitrogen 35mg / L, dissolved phosphorus 131ug / ml, Fat 7.1g / L. Then, under the protection of the flame, the cultured Actinomyces aureus mother bottle fermentation broth was prepared at a rate of 2.5 / m 3 The amount of inoculum was transferred to the primary seed medium for primary seed cultivation. The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-29°C; air flow: 30m 3 / h; the stirring speed is 70r / min; the bacterial cell concentration is 30%, and the cultivation time ...

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Abstract

The invention relates to a culture medium for producing kasugamycin by fermenting actinomyces microaureus and a culture method. According to the culture medium and the culture method, disclosed by the invention, a seed culture medium for producing the kasugamycin by fermenting the actinomyces microaureus, a carbon source and a nitrogen source in a fermentation culture medium and the optimal matching proportion are confirmed; particularly, oil sludge is added in the culture medium, so that the using amount of carbon source cottonseed oil is reduced; fermented dried bean curd dregs are added in the fermentation culture medium, so that the using amount of chrysalis oil is reduced. By utilizing a culture medium formula and a fermentation control technology which are provided in the invention, the fermentation unit of the kasugamycin can be increased, the fermentation cost can be reduced, the influence of raw auxiliary materials on the fermentation unit can be reduced to the maximum, the sources of the raw auxiliary materials are not influenced by the environment, sufficient supply of the kasugamycin is ensured, and stable and efficient production of the kasugamycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a culture medium and a culture method for fermenting and producing kasugamycin by Actinomyces aureus. Background technique [0002] Kasugamycin is a weakly alkaline antibiotic produced by Streptomyces. It is named Little Aureus Streptomycin because the producing bacteria secrete aureus in the culture medium. Kasugamycin is a dual-use antibiotic for agriculture and medicine. [0003] At present, domestic fermentation production of kasugamycin adopts a three-stage fermentation mode. The main problems of this method are as follows: [0004] 1 The fermentation level of industrialized large-scale production is low, and its fermentation level is maintained at about 10000-12000u / L. [0005] 2 There is a lack of literature reports on the complete fermentation process of kasugamycin in China, and the relevant literature reports are mainly experiments, which cannot provide effectiv...

Claims

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Application Information

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IPC IPC(8): C12P19/46C12N1/20C12R1/465
CPCC12P19/46C12N1/20
Inventor 任勇王隽梅
Owner 宁夏泰瑞制药股份有限公司
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