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A chip for microbial oil and gas exploration and its application

A microbial and oil-gas technology, applied in the chip field of microbial oil and gas exploration, can solve the problem of unculturable microorganisms, and achieve the effects of saving time for bacterial culture, high accuracy, and easy operation

Active Publication Date: 2020-06-30
ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical means based on molecular biology can overcome the technical shortcomings of the culture-based microbial technology means that most microorganisms cannot be cultivated, but there are no reports on gene chips related to oil and gas development.

Method used

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  • A chip for microbial oil and gas exploration and its application
  • A chip for microbial oil and gas exploration and its application
  • A chip for microbial oil and gas exploration and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Entrust the service company to synthesize probes for the probe sequence of SEQ ID NO: 1-92, and use the crystal core TM16 spotting instrument to spot the probes on the crystal core Polymer three-dimensional substrate to complete the preparation of the chip;

[0042] Collect samples from an exploration area to be evaluated. Samples are collected inside and outside the target area to be evaluated. The sample collection depth is 20cm. After the samples are sent back to the laboratory, FastDNA Spin Kit for Soil soil DNA extraction kit for soil DNA extraction;

[0043] Use the Agilent SureTag DNA Labeling Kit to fluorescently label the extracted DNA, and use the QiagenQIAquick Kit to purify the labeled DNA;

[0044] Use the Agilent Oligo aCGH / ChIP-Chip Hybridization Kit hybridization kit to hybridize the purified labeled product with the prepared chip. The hybridization temperature is 65 degrees Celsius. The chip is cleaned;

[0045] Use the Agilent G4900DA micro array sc...

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PUM

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Abstract

The invention relates to the technical field of biological detection and particularly relates to a chip for microbial prospecting of oil and gas and application thereof. The invention provides a probe set, a chip and a kit for prospecting of oil and gas and a prospecting method. The probe set has good specificity according to the design of a microbial 16s rRNA gene as well as an alkB gene, an alkH gene, an alkJ gene, an alkK gene, a pmoA gene, an mmoX gene or a BmoX gene. By applying the primer set or the chip to prospecting of oil and gas resources, the flora culture time of the conventional method is saved, the operation is simple and convenient, and the accuracy is high. A more reliable basis can be provided for prospecting of oil and gas resources.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a chip for microbial oil and gas exploration and its application. Background technique [0002] Exploration of oil and gas resources is a high-risk commercial activity. Due to the complexity of underground geological conditions, the success rate of oil and gas exploration and drilling in the industry has been maintained at about 30%. During the development of oil and gas exploration technology, a set of Based on petroleum geology research, it is an exploration technology system based on geophysics, geochemistry, and drilling technologies. However, this technology system has technical defects such as high exploration costs and the inability to determine the oil and gas of the target. It can be further improved. Technologies with a success rate of exploration for oil and gas resources have always been the needs of the industry. [0003] Oil and gas microbial explorati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01C12R1/265C12R1/365C12R1/38C12R1/06C12R1/465C12R1/07
CPCC12Q1/689
Inventor 邓诗财郝纯梅海张勇褚厚娟
Owner ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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