Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells

A photoelectrochemical, cell surface technology, used in measurement devices, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of expensive instruments, high biological damage, complicated operations, etc., and achieve low cost and high detection sensitivity. , the effect of simple operation

Inactive Publication Date: 2016-10-12
UNIV OF JINAN
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  • Application Information

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Problems solved by technology

The current methods for polysaccharide expression analysis (mass spectrometry, nuclear magnetic resonance, high performance liquid chromatography, etc.) have disadvantages such as complicat

Method used

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  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells
  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells
  • Preparation of photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells

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Embodiment 1

[0039] Application of paper-based photoelectrochemical devices in detecting the expression of polysaccharides on the surface of MCF-7 cells:

[0040] (1) Use Adobe illustrator CS4 software to design the printing pattern of the paper chip on the computer;

[0041] (2) Print the printing pattern designed in step (1) on No. 1 chromatographic paper with a wax printer, then place the A4 chromatographic paper with the wax pattern in a flat heater or an oven, and heat it at 130 ºC for 2 min , so that the wax melts and saturates the entire thickness of the paper, forming a hydrophobic wall;

[0042] (3) Print the carbon working electrode, Ag / AgCl reference electrode and carbon counter electrode on the wax-printed chromatographic paper obtained in step (2) by screen printing technology;

[0043] (4) After adding gold to the sample plate working area of ​​the chromatographic paper obtained in step (3), connect photoelectric materials zinc oxide, cadmium telluride quantum dots, and gold...

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Abstract

The invention discloses a preparation method of a photo-induced chemical paper chip for detecting expression of polysaccharides on surfaces of cancer cells. The preparation method comprises the following steps: designing lyophobic wax printing patterns of the paper chip by using Adobe illustrator CS4 software; printing the paper chip by using a wax printer; printing a working electrode, a reference electrode and a counter electrode by using a screen printing technique; growing gold nanoparticles in a working region, then connecting photoelectric material zinc oxide, cadmium telluride quantum dots and gold-grafted meso-porous silicon, modifying HRP-mdsDNA, and providing a built-in light source and binding sites of recognition cells for a photo-induced chemical system; and finally, adding a polysaccharides inhibitor, and detecting expression of the polysaccharides on the surfaces of the tumor cells by change of photocurrent response.

Description

technical field [0001] The invention relates to the technical fields of nanomaterial technology, hybridization chain reaction, paper chip technology, and polysaccharide expression detection on the surface of tumor cells, and more specifically relates to the construction of a paper-based analysis device suitable for in situ detection of polysaccharide expression on the surface of tumor cells. Background technique [0002] Glycosylation is a significant modification of proteins that can regulate protein functions. Polysaccharides are important structures on the cell surface and are closely related to cell migration and differentiation. The expression of polysaccharides on the cell surface is a dynamic process closely related to the cell environment and state. The abnormal expression of polysaccharides is not only related to many pathological phenomena but also a marker of many diseases. Therefore, detecting the expression of polysaccharides on the cell surface is of great si...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 葛慎光兰飞飞梁琳琳张彦李丽杨红梅任娜于京华颜梅
Owner UNIV OF JINAN
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