Simple method for rapidly detecting physalospora piricola infection amount in apple branch
A technology of ring ring bacteria and apple branches, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the difficulty of knowing the exact amount of bacteria carried by trees, researchers difficult to control the effect of pathogenic bacteria agents, and restrict the epidemiology of ring ring disease and prevention and control technology research and development, to achieve the effect of simple operation method and reliable detection results
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[0022] Application case 2: The detection rate of pathogenic bacteria after inoculation at different times: at the end of July 2014, use 10 per ml 5 The conidia suspension of a ringworm fungus was inoculated into branches of Fuji apples with a diameter of 0.3-1.0 cm, and covered with plastic bags to keep them moist for 24 hours after inoculation; the inoculated branches were marked and divided into 4 groups on average, and 7 days after inoculation respectively. After 1, 14, 21 and 28 days, girdling was performed and treated with paraquat, 24 shoots were treated at each time. From the 5th day after paraquat treatment, the number of shoots with necrotic cortex and the number of shoots with pycnidia of R. verticillium were checked and recorded.
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