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Primers and method for identifying diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater

A technology for activated sludge and saponified wastewater, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc. It can solve the problems of inability to accurately identify and complex microbial community structure in activated sludge, etc. Achieve the effect of simple steps

Inactive Publication Date: 2016-10-26
UNIV OF JINAN
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Problems solved by technology

[0005] Although the above-mentioned identification method can identify the species information of the tested algae strain, due to the complex structure of the microbial community in the activated sludge, the above-mentioned method cannot be accurately identified, and most of the microorganisms cannot be isolated and cultured by the traditional method. It is difficult to accurately analyze the sludge microbial community through the enrichment, separation and cultivation of

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  • Primers and method for identifying diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater
  • Primers and method for identifying diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater
  • Primers and method for identifying diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater

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Embodiment 1

[0060] 1. NCBI search nucleotide sequence. Log on to www.ncbi.nlm.nih.gov / Gen-Bank, you can get the gene sequence from Gen Bank as a template.

[0061] The target amplification group of the present invention is eukaryotic algae, according to Burki (2008) to photosynthetic eukaryote, Adl (2012) to the molecular system taxonomy of eukaryote, the target amplification group is refined as green algae (Chlorophyceae) , Porphyridium, Euglena, Charophyta, Pyrrophyta, Cryptophyceae, Haptophyta, Chlorarachniophyta, Chrysophyceae, Diatom ), brown algae (Phaeophyta), yellow algae (Xanthophyta) and other 12 eukaryotic algae. Determine the DNA sequence that needs to be amplified according to the test requirements, and know the sequence of its coding structural gene region. The specific method is as follows:

[0062] Select Nucleotide in the Search dialog box, fill in the name of the Nucleotide to be searched in the dialog box, such as input Chlorophyceae (green algae), click search to get...

Embodiment 2

[0080] The activated sludge of propylene oxide saponification wastewater described in this example is obtained from the activated sludge in the treatment process of propylene oxide saponification wastewater of Binhua Group Co., Ltd.

[0081] A method for identifying the diversity of eukaryotic algae in the activated sludge of propylene oxide saponification wastewater, comprising the steps:

[0082] (1) get propylene oxide saponification wastewater activated sludge sample, extract total DNA;

[0083] The activated sludge is taken from the activated sludge of the sewage treatment plant of Shandong Binhua Group, which is the activated sludge for the treatment of propylene oxide saponification wastewater. The fresh sludge taken from the sewage treatment plant was centrifuged at 4000r / min at 4°C for 10min, and the supernatant was discarded and stored in a refrigerator at 4°C. According to the method described in Example 1 in Chinese patent document CN103789300A (application number...

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Abstract

The invention relates to primers and a method for identifying the diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater. There is a pair of the primers. The upstream primer nucleotide sequence is as shown in SEQ ID NO. 1, and the downstream primer nucleotide sequence is as shown in SEQ ID NO. 2. The invention also relates to a method for identifying the diversity of eukaryotic algae in activated sludge of epoxypropane saponified wastewater. By the design of the eukaryotic algae-specific primers, metagenome DNA of the activated sludge of epoxypropane saponified wastewater can undergo specific amplification to obtain different eukaryotic algae. Then, eukaryotic algae species in the activated sludge can be identified.

Description

technical field [0001] The invention relates to a primer and a method for identifying the diversity of eukaryotic algae in the activated sludge of propylene oxide saponification wastewater, belonging to the field of biotechnology. Background technique [0002] Propylene oxide saponification wastewater is a large amount of sewage produced during the production of propylene oxide by the chlorohydrin method. The wastewater has the characteristics of high pH value, high calcium chloride content, and high COD, and contains a large amount of chlorides that are difficult to biodegrade, such as chloropropanol, dichloroisopropyl ether, and dichloropropane. This high-salt, organic-rich wastewater can cause serious pollution to the environment, and reasonable and effective ways to treat this wastewater cannot be ignored. [0003] Biologically activated sludge method is currently the main way to treat propylene oxide saponification wastewater. Activated sludge is the main body of the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6895C12Q1/6869C12Q2531/113C12Q2537/165
Inventor 李强何荣樊祥宇李玉梅古鹏飞纪雁沈峻宇
Owner UNIV OF JINAN
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