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Method for purification of taxifolin

A technology of Douglas fir and Douglas fir, which is applied in the field of separation of natural products, can solve problems such as unsuitable for industrial production, complex process, cumbersome steps, etc., and achieve the effects of increasing raw material utilization, accelerating extraction efficiency, and overcoming heavy pollution

Inactive Publication Date: 2016-11-02
南京泽朗医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method has cumbersome steps, complex process and heavy pollution, and is not suitable for industrial production

Method used

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  • Method for purification of taxifolin

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Effect test

Embodiment 1

[0020] Take Douglas fir and crush it, weigh 5kg, add 30kg of 40% ethanol solution, ultrasonically extract twice, the ultrasonic frequency is 65KHz, the ultrasonic time is 20min, filter, the filtrate is concentrated under reduced pressure, and the concentrated solution is placed on a D-101 macroporous resin column Adsorption, 3BV water → 4BV70% ethanol elutes the resin column, collects the eluate, concentrates until it has no alcohol smell, and passes through a 3000D hollow fiber polysulfone membrane ultrafiltration, controlling the inlet pressure to 0.25MPa, and the filtrate passes through a 400D hollow fiber Concentrate by nanofiltration of polysulfone membrane, control the inlet pressure to 2.0MPa, and vacuum-dry the concentrate at 75°C to obtain 4.36g of taxifolin with a content of 88.3%.

Embodiment 2

[0022] Take Douglas fir and crush it, weigh 2kg, add 20kg of 50% ethanol solution, ultrasonically extract twice, the ultrasonic frequency is 40KHz, the ultrasonic time is 30min, filter, the filtrate is concentrated under reduced pressure, and the concentrated solution is placed on an AB-8 macroporous resin column Adsorption, 4BV water → 6BV90% ethanol elutes the resin column, collects the eluate, concentrates until it has no alcohol smell, and passes through a 6000D hollow fiber polysulfone membrane ultrafiltration, controlling the inlet pressure to 0.2MPa, and the filtrate passes through a 500D hollow fiber Concentrate by nanofiltration of polysulfone membrane, control the inlet pressure to 1.0MPa, and vacuum-dry the concentrate at 75°C to obtain 2.16g of taxifolin with a content of 89.4%.

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Abstract

The invention relates to a method for purification of taxifolin. The process steps are as below: (1) crushing Douglas fir, adding ethanol solution, carrying out ultrasonic extraction, filtering and conducting vacuum concentration on the filtrate; (2) passing the concentrate through a macroporous resin adsorption column, washing by water to remove impurity, eluting the resin column by ethanol, and collecting alcohol eluent; and (3) concentrating the eluent to a state without alcohol taste, conducting ultrafiltration, nanofiltration condensation, and drying to obtain taxifolin. The method has the advantages of simple process, low cost, less pollution and good quality, and is an environment-friendly purification method.

Description

technical field [0001] The invention belongs to the field of separation of natural products and relates to a method for purifying taxifolin. Background technique [0002] Taxifolin, also known as taxifolin, toxin, comes from Douglas fir, a pinaceae plant, with a molecular formula of C 15 h 12 o 7 , the molecular weight is 304.25, and the structural formula is as follows: [0003] [0004] Taxifolin is a colorless needle crystal, easily soluble in ethanol, acetic acid, boiling water, slightly soluble in cold water, almost insoluble in benzene. [0005] Pharmacological effects of Taxifolin: (1) Anti-cancer: It is active against mouse leukemia P388, and the life extension rates of 150 and 100 mg / kg are 40% and 37% respectively; (2) Antibacterial: Against Staphylococcus aureus, large intestine Bacillus, Shigella and Salmonella typhi have strong antibacterial effect. In addition, taxifolin, as a powerful natural antioxidant, can be used as a preservative in the food indus...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 杨成东
Owner 南京泽朗医药技术有限公司
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