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Simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium

A Brassica and low accumulation technology is applied in the field of screening excellent Brassica plant germplasm materials with low accumulation of heavy metal cadmium, and can solve the problems of difficult breeding populations and germplasm resource material screening, complex sample preparation, and high detection costs , to achieve the effect of speeding up the screening process, low cost and shortening the screening cycle

Active Publication Date: 2016-11-09
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The traditional methods for screening the accumulation of heavy metal cadmium in Brassica plants mainly use graphite furnace atomic absorption spectrometry, flame atomic absorption spectrometry and inductively coupled plasma emission spectrometry to measure cadmium content. These methods have the advantage of high precision; but , the detection cost is very high, and the instrument operation and sample preparation are also extremely complicated, making it difficult to carry out large-scale screening of breeding populations and germplasm resources.

Method used

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  • Simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium
  • Simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium
  • Simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium

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Experimental program
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Effect test

Embodiment 1

[0053] To measure the Cd of 8 Brassica napus varieties in the control (double distilled water treatment) and 50mg / L 2+ The length of the radicle at the cotyledon stage after the solution treatment, using the cadmium damage coefficient as an index to evaluate the inhibitory effect on the length of the radicle at the cotyledon stage of the Brassica napus under the stress of the cadmium solution, and grade the inhibitory effect, and screen out the Brassica oleracea The kind of low accumulation heavy metal cadmium in the rape variety, the specific embodiment of the present invention is described; Then measure the cadmium accumulation in the cotyledons of the rapeseed rape seed cadmium stress of trying, statistically analyze the accumulation of heavy metal cadmium in the rapeseed cotyledons of different classifications Rule, to verify the screening results of the present invention.

[0054] 1. The specific implementation plan for screening low-accumulation cadmium Brassica napus vari...

Embodiment 2

[0091] To measure the Cd of 8 cabbage varieties in the control (double distilled water treatment) and 30mg / L 2+ The length of the radicle at the cotyledon stage after the solution treatment, using the cadmium damage coefficient as an index to evaluate the inhibitory effect on the length of the radicle at the cotyledon stage of the tested Chinese cabbage under the stress of the cadmium solution, and grade the inhibitory effect, and screen out the middle and low levels of the tested Chinese cabbage varieties. Accumulate the kind of heavy metal cadmium, the specific embodiment of the present invention is described; Then measure the amount of cadmium accumulation in the cotyledon of the cabbage seed cadmium stress of the test, and statistically analyze the accumulation rule of heavy metal cadmium in the cabbage cotyledon of different classifications, to verify the screening of the present invention result.

[0092] 1. The specific implementation plan for screening low-accumulation...

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Abstract

The invention discloses a simple rapid high-throughput screening method of brassica plants with low accumulation of heavy metal cadmium and relates to the technical field of brassica plants breeding and screening of germplasm resource. The method comprises the following steps: (1) seed sterilization; (2) preparation of a seed germination substratum; (3) seed distribution; (4) bud forcing treatment; (5) seed germination and growth; (6) radicle length measurement; (7) evaluation of radicle length inhibition effect in cotyledon stage; and (8) screening of brassica plants with low accumulation of heavy metal cadmium from the tested group. Only by processing the tested seeds of brassica plants with a certain concentration of cadmium solution on the seed germination substratum, brassica plants with low accumulation of heavy metal cadmium can be screened out. The method is simple and labor-saving. It only takes one week in screening. Thus, screening period is greatly shortened, and screening course is accelerated. Brassica plants with low accumulation of heavy metal cadmium can be screened from a large group with high throughput. The method is not dependent on expensive measuring instruments, is low-cost and is easy for large-scale promotion and application.

Description

technical field [0001] The invention relates to the technical field of Brassica plant breeding and germplasm resource screening, in particular to a simple, rapid, high-throughput screening method for Brassica plants with low accumulation of heavy metal cadmium, which is used for screening excellent Brassica plants with low accumulation of heavy metal cadmium germplasm material. Background technique [0002] Cadmium (Cadmium, Cd) is located in group IIB of the fifth period of the periodic table of elements, and its molecular weight is 112.41. It is a silvery white shiny metal and is widely used in the chemical industry. Heavy metal cadmium is a non-essential metal element for organisms, and it is one of the heavy metal elements that are toxic to humans, animals and plants; high concentrations of heavy metal cadmium can inhibit the growth and development of plants, resulting in reduced crop yields; in addition, accumulation in crops The heavy metal cadmium in the environment ...

Claims

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Application Information

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IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 伍晓明张付贵肖欣高桂珍闫贵欣陈碧云许鲲
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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