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Commercial-use tissue culture seedling breeding method of American red maple October Glory

A technology of tissue culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, applications, etc., can solve the problems of being out of economic feasibility, unable to apply large-scale commercial production, etc., and achieve stable traits and neat seedlings Effect

Active Publication Date: 2016-11-09
江苏东郁植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to factors such as the longer physiological life of woody plants, more chromosome numbers, high degree of cortex and xylem differentiation, and the scientific research and education system, a large number of research results carried out by many research institutes in my country are separated from the economic feasibility of commercial production. Various indicators such as stability and seedling uniformity cannot be applied to large-scale commercial production

Method used

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  • Commercial-use tissue culture seedling breeding method of American red maple October Glory
  • Commercial-use tissue culture seedling breeding method of American red maple October Glory
  • Commercial-use tissue culture seedling breeding method of American red maple October Glory

Examples

Experimental program
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Effect test

Embodiment 1

[0050] (1) selection of explants: selecting the non-lignified tender shoots of 4-year-old seedlings in the spring of natural germination with the age of greenhouse growth, the tender shoots taking materials length is 15cm, with 4 internodes; remove leaves and overlong petioles, sterilize; The disinfection method is as follows:

[0051]Wipe the dust on the surface of the explant with alcohol cotton, then rinse it in 1:3 bleach (commercial bleach: sterile water, volume ratio) for 15 minutes, shake it gently 4 times during this period, and then rinse in sterile water 3 times, let stand for 2 minutes, then use sterile filter paper to absorb the surface water, cut off the dead part of the petiole and the upper and lower incisions of the stem segment, and retain 1 cm at the upper end and 1.5 cm at the lower end;

[0052] (2) Start-up culture: Inoculate the sterilized explants in the start-up medium, and transfer them to the culture room, cultivate at 25°C for 48 hours in dark light,...

Embodiment 2

[0079] (1) selection of explants: selection of growth years is the non-lignified tender shoots of natural germination in spring of 3-year seedlings, and the length of tender shoots is 10cm, with 3 internodes;

[0080] In addition to leaves and long petioles, disinfection; disinfection methods are as follows:

[0081] Disinfection with hydrogen peroxide, the disinfection conditions are: the concentration of hydrogen peroxide is 10%, the disinfection time is 20 minutes, and then the surface water is blotted dry on sterile filter paper;

[0082] (2) Start-up culture: Inoculate the sterilized explants in the start-up medium, and transfer them to the culture room, cultivate at 25°C for 48 hours in dark light, and then give 1500 LUX of light and a light period of 16 / 8 hours for start-up culture After 3 weeks, the dormant axillary buds germinated, and the initiation culture was completed.

[0083] The described start-up medium, with water as a solvent, comprises the following compon...

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Abstract

The invention provides a commercial-use tissue culture seedling breeding method of American red maple October Glory. The commercial-use tissue culture seedling breeding method comprises the following steps: (1) selecting individual tender shoots of nursery stock, removing leaves and too long petiole, sterilizing, and cutting dead parts; (2) inoculating the individual tender shoots into a start culture medium for culture; (3) cutting germinated axillary buds into single buds in the longitudinal directions of stems, and inoculating the buds into a solid propagating medium for propagating culture; (4) separating propagated cluster buds, and transferring into a seedling strengthening culture medium for seedling strengthening culture; (5) cutting off bottom stems of tissue culture seedlings after seedling strengthening culture, and inserting the stems into a rooting culture medium for rooting induction culture; (6) acclimatizating and transplanting the tissue culture seedlings after rooting induction culture in a greenhouse, thereby obtaining commercial seedlings. The commercial-use tissue culture seedling breeding method is a woody plant tissue culture method which is economic and feasible, stable in property, tidy in seedling and applicable to large-scale commercial production, and requirements of commercial nursery stock production can be met.

Description

technical field [0001] The invention relates to a seedling breeding method for commercial large-scale tissue culture of American red maple "October Brilliant". Background technique [0002] American red maple "October Glory", Acer rubrum 'October Glory' is a woody plant of the maple family Aeraceae, and it is one of the improved horticultural species of American red maple. It is a large deciduous tree with the fastest growth, strong adaptability, cold resistance, drought resistance and humidity resistance. Acidic to neutral soils enhance fall colors. Strong resistance to harmful gases, especially strong absorption of chlorine. It is widely used for planting in parks, communities, and streets. It can be used for both garden landscaping and street trees. It is deeply loved by people. It is also the only coleus species that can be used as a street tree. At present, American red maple "October Brilliant" seedlings can be obtained through cutting propagation and seeding propa...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 胡春宏常苹王婷婷季翔
Owner 江苏东郁植物科技有限公司
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