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Method for supercritical CO2 extraction of polysaccharide in grifola frondosus

A Grifola frondosa, supercritical technology, applied in the production of bulk chemicals, can solve the problems of complex extraction process steps, can not meet the manufacturers, long production cycle, etc., to achieve enhanced solubility and selectivity, perfect reaction, low cost Effect

Inactive Publication Date: 2016-11-09
JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the invention is to provide a supercritical CO 2 The method for extracting polysaccharides in Grifola frondosa solves the problem that the extraction method of polysaccharides in Grifola frondosa in the prior art has complex extraction process steps, long production cycle and high cost, and cannot meet the needs of manufacturers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A supercritical CO 2 The method for extracting Grifola frondosa polysaccharides comprises the following steps:

[0020] (1) Select fresh fruit bodies of Grifola frondosa with plump flesh, no mildew, and no insect erosion, and dry them until the water content is 5%, then use a pulverizer to crush them, and pass through a 80-mesh sieve to obtain Grifola frondosa powder .

[0021] (2) Using CO 2 As a solvent, ethanol solution with a concentration of 60% was used as an entrainer, and the mass ratio of the entrainer to Grifola frondosa powder was 1:1.

[0022] (3) Spray the entrainer evenly on the pulverized Grifola frondosa powder, add it into a supercritical extraction kettle for extraction, the pressure of the extraction kettle is 20MPa, the temperature is 30℃, CO 2 The flow rate was controlled at 5 L / h, and the extraction time was 100 min to obtain the extract.

[0023] (4) Add distilled water to the extract obtained by extraction and separation. The volume ratio of ...

Embodiment 2

[0027] A supercritical CO 2 The method for extracting Grifola frondosa polysaccharides comprises the following steps:

[0028] (1) Select fresh fruiting bodies of Grifola frondosa with plump flesh, no mildew, and no insect erosion, and dry them until the water content is 10%, then crush them with a pulverizer, and pass through an 80-mesh sieve to obtain Grifola frondosa powder .

[0029] (2) Using CO 2 As a solvent, ethanol solution with a concentration of 95% was used as an entrainer, and the mass ratio of the entrainer to Grifola frondosa powder was 2:1.

[0030] (3) Spray the entrainer evenly on the pulverized Grifola frondosa powder, add it into a supercritical extraction kettle for extraction, the pressure of the extraction kettle is 40MPa, the temperature is 55℃, CO 2 The flow rate was controlled at 20 L / h, and the extraction time was 180 minutes to obtain the extract.

[0031] (4) Add distilled water to the extract obtained by extraction and separation. The volume r...

Embodiment 3

[0035] A supercritical CO 2 The method for extracting Grifola frondosa polysaccharides comprises the following steps:

[0036] (1) Select fresh fruit bodies of Grifola frondosa with plump flesh, no mildew, and no insect erosion, and dry them until the moisture content is 7%, then use a pulverizer to crush them, and pass through an 80-mesh sieve to obtain Grifola frondosa powder .

[0037] (2) Using CO 2 As a solvent, ethanol solution with a concentration of 60% was used as an entrainer, and the mass ratio of the entrainer to Grifola frondosa powder was 1.5:1.

[0038] (3) Spray the entrainer evenly on the pulverized Grifola frondosa powder, add it into a supercritical extraction kettle for extraction, the pressure of the extraction kettle is 30MPa, the temperature is 42°C, CO 2 The flow rate was controlled at 12 L / h, and the extraction time was 140 min to obtain the extract.

[0039](4) Add distilled water to the extract obtained by extraction and separation. The volume ra...

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Abstract

A method for supercritical CO2 extraction of polysaccharide in grifola frondosus is provided. The extraction method comprises the following steps: selecting fresh grifola frondosus fruiting bodies, drying, crushing, and sieving, to obtain a grifola frondosus powder; taking CO2 as a solvent, taking an ethanol solution with the concentration of 60-95% as an entrainer, performing extraction, wherein the pressure of an extraction kettle is 20-40 MPa, the temperature is 30-55 DEG C, the CO2 flow speed is controlled to be 5-20 L / h, and the extraction time is 100-180 min; purifying an extract liquid obtained from extraction and separation with a composite enzyme, then adding ethanol to the concentration of 90%, carrying out static alcohol precipitation for 24 h, and centrifuging for 20 min at the speed of 4000 r / min, to obtain a precipitate; carrying out vacuum drying of the precipitate under the conditions of the pressure of 0.4-0.8 MPa and the temperature of 36 DEG C, and thus obtaining a dry substance of grifola frondosus polysaccharide with the water content of less than 8%. The extraction operation is convenient, the efficiency is high, the process is simple, the production efficiency is greatly improved, and the method is suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from Grifola frondosa, in particular to a supercritical CO 2 A method for extracting polysaccharides from Grifola frondosa. Background technique [0002] Grifola frondosa is a precious edible and medicinal fungus with great development value. , Thousand Buddha mushrooms, chestnut mushrooms, cloud mushrooms, etc., which are called Maitake mushrooms in Japan. [0003] What people pay more attention to in Grifola frondosa is its physiologically active substance - polysaccharide. Polysaccharide is a kind of fungal polysaccharide isolated from the fruiting body of Grifola frondosa. The study of fungal polysaccharide as a biological response regulator has been deeply carried out at home and abroad, and the remarkable curative effect of Grifola frondosa polysaccharide has gradually been recognized by people. After a lot of chemical and pharmacological analysis, it is found that the polysaccha...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0024Y02P20/54
Inventor 仲延龙朱惠芬
Owner JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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