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Fermenting method for joint production of alpha-ketoglutaric acid and pyruvic acid

A technology of ketoglutaric acid and fermentation method, applied in the field of fermentation engineering, can solve the problems of adding new impurities to sulfuric acid, low substrate conversion rate, prolonged fermentation period, etc., to promote the accumulation of carboxylic acid, improve economic benefits, shorten the The effect of the fermentation cycle

Active Publication Date: 2016-11-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this has the following disadvantages: prolonged fermentation period, addition of sulfuric acid will increase new impurities, and low substrate conversion rate; in industrial production, these disadvantages will be magnified and bring inconvenience to production

Method used

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  • Fermenting method for joint production of alpha-ketoglutaric acid and pyruvic acid
  • Fermenting method for joint production of alpha-ketoglutaric acid and pyruvic acid
  • Fermenting method for joint production of alpha-ketoglutaric acid and pyruvic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Carboxylic acid co-production under the condition of initial glycerol concentration 50g / L of embodiment 1 and adding glycerol later stage

[0024] Step 1. Inoculate Yarrowia lipolytica WSH-Z06 from a glycerol tube to the slant of an eggplant bottle for activation. After culturing at 28°C for 24 hours, use an inoculation loop to connect the activated seeds to the first-level seed medium, and incubate at 28°C for 18 hours. Finally, connect the primary seed solution to the secondary seed medium at 0.5% inoculation amount, and after cultivating at 28°C for 18 hours, then transfer the secondary seed liquid to the fermentation medium at 10% inoculation amount, and ferment at 28°C to cultivate. The primary seed cultivation method is: 50mL of liquid in a 500mL Erlenmeyer flask, cultivation temperature of 28°C, rotational speed of 200r / min, cultivation for 17-18h. The secondary seed cultivation method is as follows: 10.5L liquid volume in 15L seed tank, 0.5% inoculum size, 28°C...

Embodiment 2

[0028] Carboxylic acid coproduction under the condition of embodiment 2 initial glycerol concentration 150g / L

[0029] Step 1. Inoculate Yarrowia lipolytica WSH-Z06 from a glycerol tube to the slant of an eggplant bottle for activation. After culturing at 28°C for 24 hours, use an inoculation loop to connect the activated seeds to the first-level seed medium, and incubate at 28°C for 18 hours. Finally, connect the primary seed solution to the secondary seed medium at 0.5% inoculation amount, and after cultivating at 28°C for 18 hours, then transfer the secondary seed liquid to the fermentation medium at 10% inoculation amount, and ferment at 28°C to cultivate.

[0030] Step 2, in the early stage of fermentation, rely on the calcium carbonate of 10g / L in the fermentation medium as the pH buffering agent, when the pH naturally drops to 3.0, use 20% Ca(OH) 2 The solution pH was maintained at 3.0.

[0031] During the fermentation process, the initial glycerol concentration was 1...

Embodiment 3

[0033] Example 3 Only α-ketoglutaric acid is produced under the condition of initial glycerol concentration of 50g / L and adding glycerol later

[0034] Step 1. Inoculate the bacteria from the glycerol tube to the slope of the eggplant bottle for activation. After culturing at 28°C for 24 hours, use an inoculation loop to connect the activated seeds to the primary seed medium. After cultivating at 28°C for 18 hours, inoculate the The liquid was received into the secondary seed culture medium at 0.5% inoculation amount, and after culturing at 28°C for 18 hours, the secondary seed liquid was received at 10% inoculum amount in the fermentation medium for fermentation and cultivation, and the fermentation was completed after 144 hours of fermentation at 28°C.

[0035] Step 2, relying on 10g / L calcium carbonate in the fermentation medium as a pH buffer in the early stage of fermentation, when the pH naturally drops to 3.0, use 20% Ca(OH) 2 Control the pH and maintain it at 3.0. When...

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Abstract

The invention discloses a fermenting method for joint production of alpha-ketoglutaric acid and pyruvic acid, and belongs to the technical field of fermenting engineering. The fermenting method is characterized in that because the difference of commercial values of alpha-ketoglutaric acid and pyruvic acid is small, the two types of carboxylic acids are jointly produced; in the fermenting process for joint production of carboxylic acids, the proper initial concentration and flowing accelerated speed of glycerinum are controlled, so as to promote the growth of bacteria and the accumulation of carboxylic acids. The fermenting method has the advantages that the conversion rate of substrate is greatly improved, the output of total acids is increased, and the production intensity is improved; the fermenting cycle is shortened, the energy-saving and consumption-decreasing effects are realized, and the economic benefit is improved.

Description

technical field [0001] The invention relates to a fermentation method for the joint production of α-ketoglutaric acid and pyruvic acid, belonging to the technical field of fermentation engineering. Background technique [0002] α-ketoglutaric acid (α-KG for short) and pyruvic acid (Pyruvic acid) are two important short-chain carboxylic acid molecules in the process of cell metabolism, both of which can be widely used in food, medicine, chemical industry, etc. field. At present, these two organic acids are mainly synthesized by chemical methods in industrial production, but there are disadvantages such as the use of toxic substances and low yields in chemical synthesis, which limit the use of the products in the fields of medicine and food. Microbial fermentation can use a variety of renewable carbon sources to produce a variety of products, which can not only improve product safety, but also reduce pollution in the production process. [0003] Y. lipolytica (Y. lipolytica)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/50C12P7/40C12R1/645
CPCC12P7/40C12P7/50
Inventor 周景文陈坚张海林堵国成刘松方芳
Owner JIANGNAN UNIV
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