Method for preparing feruloyl oligosaccharides through rice bran fermentation

A technology for fermentation of feruloyl oligosaccharides and rice bran, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reducing costs, eliminating separation and purification processes, and improving productivity

Inactive Publication Date: 2016-11-09
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are few research reports on the preparation of FOs by fermentation, and there is no research on the preparation of FOs by using rice bran as raw material and edible fungi as fermentation strains at home and abroad.

Method used

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  • Method for preparing feruloyl oligosaccharides through rice bran fermentation
  • Method for preparing feruloyl oligosaccharides through rice bran fermentation
  • Method for preparing feruloyl oligosaccharides through rice bran fermentation

Examples

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Effect test

Embodiment 1

[0027] Example 1 Preparation of feruloyl oligosaccharides by fermenting rice bran with yellow mushroom

[0028] Mother medium (PDA): 200g of potatoes, washed and peeled, added with 1000ml of water, boiled for 30min, filtered through gauze, added with 15g of glucose, fully dissolved and packed into Erlenmeyer flasks, sterilized at 121°C for 20min, taken out and cooled for later use.

[0029] After the rice bran is dried or air-dried naturally, it is crushed and sieved through an 80-mesh sieve.

[0030] Fermentation medium: 50g rice bran plus 1L water, 15g glucose, 15g lactose, 15g peptone, 1gKH 2 PO, 1gMgSO 4 ·7H 2 O, 0.1gVB 1 .

[0031] Cultivation of the mother species: inoculate 1 g of the mother species of Pleurotus ulmoides into Erlenmeyer flasks with a liquid volume of 50 mL / 250 mL, and culture for 6 days at 25°C and at a shaker speed of 100 r / min.

[0032] Inoculate 11% (or 4-10 billion cfu / L medium) in a conical flask with a filling volume of 60 mL / 250 mL, and cult...

Embodiment 2

[0047] Example 2 Preparation of feruloyl oligosaccharides by fermenting solid rice bran with yellow mushroom

[0048] A method for preparing feruloyl oligosaccharides by fermenting rice bran,

[0049] (1) After the rice bran is dried or naturally air-dried, it is crushed and sieved through an 80-mesh sieve to make a solid medium;

[0050] (2) inoculate and ferment ferulite chrysanthemum and carry out solid fermentation for 90-120 days to obtain feruloyl oligosaccharides; the inoculum amount of the edible fungus is 8-18 billion cfu / Kg culture medium.

Embodiment 3

[0051] Example 3 Preparation of feruloyl oligosaccharides by fermenting rice bran with yellow mushroom

[0052] A method for preparing feruloyl oligosaccharides by fermenting rice bran is carried out according to the following steps:

[0053] (1) Preparation of rice bran liquid: after the rice bran is dried or naturally air-dried, crushed and sieved through an 80-mesh sieve, added to water and stirred to prepare a rice bran liquid with a solid content of 65 g / L;

[0054] (2) Configure culture medium: add 15g glucose, 8g nano lapis lazuli powder, 15g lactose and 15g peptone to 1L rice bran liquid;

[0055] (3) Fermentation preparation: Use the culture medium prepared in step (2) to inoculate the yellow elm mushroom for liquid fermentation, control the initial fermentation pH to 4, the fermentation temperature is 25°C, the shaking table speed is 100r / min, and ferment for 8 days to obtain feruloyl Oligosaccharides; the inoculation amount of the yellow elm mushroom is 6 billion c...

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Abstract

The invention discloses a method for preparing feruloyl oligosaccharides through rice bran fermentation. According to the method, a rice bran culture medium is prepared through preparation of a rice bran solution, and the feruloyl oligosaccharides are prepared. The feruloyl oligosaccharides are prepared with a fermentation method, the process of enzyme production by means of microorganisms and the enzymolysis process are integrated, the separation and purification procedure in the enzymic preparation production process is omitted, the cost is reduced, and the yield is increased. Besides, the problem about treatment of hydrolysis substances in the enzymolysis process is solved through utilization of starch, protein and other ingredients in the microorganism growth process, resources are reasonably utilized, and environment pollution is reduced. The method is simple and easy to implement, the yield of the prepared feruloyl oligosaccharides is high, and purification is easy.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides by fermentation, in particular to a method for preparing feruloyl oligosaccharides by fermenting rice bran. Background technique [0002] my country is the main producer of rice, and rice production accounts for about 37% of the global rice production. Rice bran is the main by-product of rice processing. In foreign countries, rice bran is known as "a source of nutrition given by God", and the United Nations Industrial Development Organization calls rice bran "an underutilized resource", which has a very high value of health care development. Rice bran contains a variety of nutritional elements and physiologically active substances, including a certain amount of ferulic acid, which can be further prepared by enzymatic hydrolysis to obtain feruloyl oligosaccharides. Feruloyl oligosaccharides (FOs) have excellent biological activity . [0003] Feruloyl oligosaccharides are a kind of functi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12P19/12C12P19/00C12R1/645
CPCC12P19/00C12P19/04C12P19/12C12P19/14
Inventor 迟燕平李敏胡楠楠王景会姜媛媛代永刚刘香英
Owner JILIN ACAD OF AGRI SCI
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