Biological-ionic liquid combined pretreatment method of lignocellulose

A technology of lignocellulose and ionic liquid, which is applied in the field of lignocellulose raw material pretreatment, can solve the problems of low efficiency, long pretreatment time, loss and development of sugar components, etc., to simplify the process, preserve cellulose content, reduce The effect of energy consumption

Inactive Publication Date: 2016-11-09
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biological method has mild treatment conditions, no environmental pollution, and low cost, but the dis

Method used

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  • Biological-ionic liquid combined pretreatment method of lignocellulose
  • Biological-ionic liquid combined pretreatment method of lignocellulose
  • Biological-ionic liquid combined pretreatment method of lignocellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Washing, drying and pulverizing the rice straw, controlling its particle size distribution between 180 μm and 425 μm;

[0029] (2) Inoculate the LD-1 cells stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours to obtain the seed liquid of LD-1;

[0030] (3) wherein said LB liquid culture medium each composition ratio is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L, described LB slant is to add the agar of 15g / l on the basis of above-mentioned prescription;

[0031] (4) Centrifuge the LD-1 seed solution obtained in the previous step for 2 minutes at 10,000 rpm, discard the supernatant, and collect the bacteria;

[0032] (5) further inoculate the collected LD-1 thalline into lignocellulose liquid culture medium according to 10% (volume ratio) inoculum amount, and cultivate for 3 days at a temperature of 30° C. at natural pH;

[0033] (6) wherein said lignocellulose liquid culture medium each composition ratio...

Embodiment 2

[0039] (1) Washing, drying and pulverizing the rice straw, controlling its particle size distribution between 180 μm and 425 μm;

[0040] (2) Inoculate the LD-1 cells stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours to obtain the seed liquid of LD-1;

[0041] (3) Wherein said LB liquid culture medium each composition ratio is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L, described LB slant is to add the agar of 15g / l on the basis of above-mentioned formula;

[0042] (4) Centrifuge the LD-1 seed solution obtained in the previous step for 2 minutes at 10,000 rpm, discard the supernatant, and collect the bacteria;

[0043] (5) further inoculate the collected LD-1 thalline into lignocellulose liquid culture medium according to 10% (volume ratio) inoculum amount, and cultivate for 3 days at a temperature of 30° C. at natural pH;

[0044] (6) wherein said lignocellulose liquid culture medium each composition ratio is: li...

Embodiment 3

[0050] (1) Washing, drying and pulverizing the rice straw, controlling its particle size distribution between 180 μm and 425 μm;

[0051] (2) Inoculate the LD-1 cells stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours to obtain the seed liquid of LD-1;

[0052] (3) Wherein said LB liquid culture medium each composition ratio is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L, described LB slant is to add the agar of 15g / l on the basis of above-mentioned formula;

[0053] (4) Centrifuge the LD-1 seed solution obtained in the previous step for 2 minutes at 10,000 rpm, discard the supernatant, and collect the bacteria;

[0054] (5) further inoculate the collected LD-1 thalline into lignocellulose liquid culture medium according to 10% (volume ratio) inoculum amount, and cultivate for 3 days at a temperature of 30° C. at natural pH;

[0055] (6) wherein said lignocellulose liquid culture medium each composition ratio is: ...

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Abstract

The invention relates to a method for improving the lignocellulose saccharification efficiency by means of bacterium and ionic liquid combined pretreatment. The method comprises the steps that biological treatment is conducted on the lignocellulose raw materials by means of an efficient lignin degradation bacterium Sphingobacterium sp.LD-1 of which the preservation number is CGMCC No.10920, partial lignin and hemicellulose are removed, and the dense structure of the lignin is destroyed to a certain extent; pretreatment is conducted on the lignocellulose at a certain temperature for a certain period of time by means of an ionic liquid. According to the method for improving the lignocellulose saccharification efficiency by means of bacterium and ionic liquid combined pretreatment, the proportions of the lignin and the hemicellulose can be reduced, the degree of crystallinity of the lignocellulose is obviously decreased, the accessible surface of cellulase is enlarged, and the saccharification efficiency of the lignocellulose is improved. The method for improving the lignocellulose saccharification efficiency by means of bacterium and ionic liquid combined pretreatment has the advantages of being low in requirement of equipment on compression resistance and corrosion resistance, easy to operate, environmentally friendly and the like.

Description

technical field [0001] The invention belongs to the technical field of lignocellulosic raw material pretreatment, and specifically relates to a method for jointly pretreating lignocellulose raw material with bacteria Sphingobacterium sp.LD-1 and ionic liquid to improve enzymolysis efficiency. Background technique [0002] With the continuous development of the global economy and culture, people's demand for renewable energy is increasing, and lignocellulose is the most abundant natural renewable biomass resource on the earth. In recent years, the use of lignocellulose to produce fuel ethanol has attracted widespread attention from experts and scholars at home and abroad. The development of energy technologies for producing fuel ethanol from lignocellulose is of great significance for solving today's energy shortages, environmental pollution, and food crises. . [0003] Lignocellulose constitutes the cell wall of plants, which is mainly composed of three parts: cellulose, he...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12R1/01
CPCC12P19/14C12P2201/00
Inventor 陈跃辉周沫戴友芝
Owner XIANGTAN UNIV
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