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Electrochemical sensor, preparation method and application thereof in quick detection of aflatoxin B1 (AFB1)

A sensor and electrochemical technology, applied in the direction of electrochemical variables of materials, can solve the problems of unfavorable long-term storage, high cost of antibody preparation, strict environmental requirements, etc. Effect

Inactive Publication Date: 2016-11-09
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these immunological-based methods require long-term pretreatment of the electrode. Using the antigen-antibody immunoaffinity method, the cost of antibody preparation is high, and the environmental requirements are relatively strict, which is not conducive to long-term storage.

Method used

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  • Electrochemical sensor, preparation method and application thereof in quick detection of aflatoxin B1 (AFB1)
  • Electrochemical sensor, preparation method and application thereof in quick detection of aflatoxin B1 (AFB1)
  • Electrochemical sensor, preparation method and application thereof in quick detection of aflatoxin B1 (AFB1)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Assembly of the electrochemical sensor of the present invention and a schematic diagram of the detection process of AFB1, such as figure 1 Shown; detailed implementation is as follows:

[0039] (1) Electrode pretreatment: 1.0, 0.3 and 0.05 µm Al 2 o 3 The gold electrode was polished to a mirror surface with powder, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0040] (2) Preparation of buffer: prepare 0.2 M and 5 mM K respectively 2 HPO 4 solution with 0.2 M and 5 mM KH 2 PO 4 solution, mix the two solutions of the same concentration and adjust the pH to 7.0, prepare 0.2 M PB and 5 mM PB for storage.

[0041] (3) Preparation of methylene blue (MB) solution: Weigh 0.0224 g MB solid sample, dissolve it with the 5 mM PB solution prepared in step (2), and dilute to 100 mL to obtain 0.6 mM MB stock solution.

[0042] (4) Preparation of ssDNA solution: HS-ssDNA was prepared with 0.2 M PB prepared in step...

Embodiment 2

[0052] (1) Electrode pretreatment: 1.0, 0.3 and 0.05 µm Al 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0053] (2) Preparation of buffer: prepare 0.2 M and 5 mM K respectively 2 HPO 4 solution with 0.2 M and 5 mM KH 2 PO 4 solution, mix the two solutions of the same concentration and adjust the pH to 7.0, prepare 0.2 M PB and 5 mM PB for storage.

[0054] (3) Preparation of methylene blue (MB) solution: Weigh 0.0224 g MB solid sample, dissolve it with the 5 mM PB solution prepared in step (2), and dilute to 100 mL to obtain 0.6 mM MB stock solution.

[0055] (4) Preparation of ssDNA solution: HS-ssDNA was prepared with 0.2 M PB prepared in step (2) to prepare HS-ssDNA solution with a concentration of 100 µM; its complementary sequence was prepared with 0.2 M PB buffer solution prepared in step (2) Prepare target DNA solutions with a concentration of 4 µM. ...

Embodiment 3

[0063] (1) Electrode pretreatment: 1.0, 0.3 and 0.05 µm Al 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0064] (2) Preparation of buffer: prepare 0.2 M and 5 mM K respectively 2 HPO 4 solution with 0.2 M and 5 mM KH 2 PO 4 solution, mix the two solutions of the same concentration and adjust the pH to 7.0, prepare 0.2 M PB and 5 mM PB for storage.

[0065] (3) Preparation of methylene blue (MB) solution: Weigh 0.0224 g MB solid sample, dissolve it with the 5 mM PB solution prepared in step (2), and dilute to 100 mL to obtain 0.6 mM MB stock solution.

[0066] (4) Preparation of ssDNA solution: HS-ssDNA was prepared with 0.2 M PB prepared in step (2) to prepare HS-ssDNA solution with a concentration of 100 µM; its complementary sequence was prepared with 0.2 M PB buffer solution prepared in step (2) Prepare target DNA solutions with a concentration of 4 µM. ...

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Abstract

The invention discloses an electrochemical sensor, a preparation method and application thereof in quick detection of aflatoxin B1 (AFB1). The electrochemical sensor comprises a three-electrode system and a detection cell, wherein the three-electrode system comprises a working electrode, an Ag / AgCl reference electrode and a platinum wire counter electrode, and the working electrode is a modified gold electrode; the detection cell is filled with a base solution formed by an electroactive indicator and a buffer solution; the electrodes are modified by utilizing DAN to perform electrochemical detection on AFB1, expensive monoclonal AFB1 antibody is not needed, the problems of complicated operation, high cost, high sample amount, long time consumption and the like of general chromatography and immunological detection methods can be solved, and instant detection of AFB1 is realized. The electrochemical sensor is convenient and quick, has the advantages of high detection sensitivity, 10 ng / mL low detection limit, small required sample amount, extremely low detection cost, small interference and the like, and can be used for quickly, easily, conveniently and accurately quantitatively detecting AFB1.

Description

technical field [0001] The invention belongs to the technical field of food detection and biosensing, and in particular relates to an electrochemical sensor, a preparation method and its application in rapid detection of aflatoxin B1. Background technique [0002] It is estimated that 25% of the world's food crops are contaminated with mycotoxins every year. The Food and Agriculture Organization of the United Nations estimates that the world's annual economic losses caused by this are hundreds of billions of dollars. Therefore, feed mycotoxin infection has become a problem that cannot be ignored in the feed industry and animal husbandry production. [0003] There are many kinds of toxins produced by feed contamination under natural conditions, among which aflatoxin (AF) is the most representative mycotoxin in feed, and aflatoxin B1 (AFB1) is the most toxic. Aflatoxin is a highly toxic substance, produced by toxic Aspergillus flavus and Aspergillus parasitica, all livestock...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 叶永康操小栋高娇娜方俊杰孙汉臣魏兆军
Owner HEFEI UNIV OF TECH
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