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A kind of enrichment purification method of zearalenone toxoid

A technology of zearalenone and toxoids, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of simultaneous purification of multiple toxins, expensive, time-consuming and laborious, etc., and achieve stable purification methods and low prices , good purification effect

Active Publication Date: 2018-11-23
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

However, commercial solid-phase extraction columns are expensive, and most of them are aimed at the purification of a certain toxin, and cannot achieve the purpose of purifying multiple toxins at the same time, and most solid-phase extraction columns require multi-step operations such as sample loading, rinsing, and elution. The process is cumbersome and time-consuming
At present, there is still a lack of an inexpensive, easy-to-operate, and rapid enrichment and purification method that can simultaneously and effectively enrich and purify the six zearalenone toxins in agricultural products

Method used

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  • A kind of enrichment purification method of zearalenone toxoid
  • A kind of enrichment purification method of zearalenone toxoid
  • A kind of enrichment purification method of zearalenone toxoid

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Embodiment 1

[0028] Implementation 1: Enrichment and purification of six zearalenone toxoids in corn

[0029] The first step is to synthesize magnetic nanomaterials: 300mg multi-walled carbon nanotubes (8nm i.d., 10-30μm length, 500m 2 / g) (Nanjing Xianfeng Nano Material Technology Co., Ltd.) was added with 250 mL of deionized water, after ultrasonication for 1 h, it was transferred to a constant temperature water bath at 60°C, and nitrogen gas was passed for 0.5 h while stirring to exhaust the oxygen in the reaction environment. After adding 0.81 g of ferric chloride hexahydrate and 0.30 g of ferrous chloride tetrahydrate to react for 0.5 h, adjust the pH to 12 with 1 mol / L sodium hydroxide solution, and continue the reaction for 2 h. During the whole reaction process, a constant temperature of 60° C., constant stirring and nitrogen gas flow were maintained. After the reaction is completed, the bottom of the container is adsorbed by a magnet, the upper layer liquid is discarded, and an a...

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Abstract

The invention provides an enrichment purification method of zearalenone toxoid. The method comprises the steps that magnetic nanoparticles synthesized by adopting multiwalled carbon nanotubes as raw materials are added into a sample extracting solution of an agricultural product, toxin adsorption is performed, a magnet is used for gathering magnetic nanoparticles containing zearalenone toxoid, a supernate is removed, a desorption solution is added for desorption, and then a target condensation solution is obtained. The enrichment purification method of zearalenone toxoid is stable, rapid, mature, applicable to simultaneous enrichment and purification of six zearalenone toxoids in corns, wide in application range and good in purification effect.

Description

technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a method for enriching and purifying zearalenone toxoids. Background technique [0002] Zearalenone toxoids are a class of mycotoxins produced by Fusarium fungi, mainly including zearalenone (ZEN) and its derivatives zearalenone (ZAN), α-zearalenol (α- ZOL), α-zearalenol (α-ZAL), β-zearalenol (β-ZOL) and β-zearalenol (β-ZAL). This type of toxin mainly exists in corn, wheat, barley, rice and other crops and their products. It has strong reproductive toxicity and can cause hyperestrogenism in animals, leading to infertility or abortion in animals, and bringing economic losses to animal husbandry. Contamination in food can cause precocious puberty in children. [0003] The existing methods for the enrichment and purification of zearalenone toxoids in food and agricultural products are mainly solid-phase extraction column purification, and commercial s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/08
CPCG01N30/06G01N30/08
Inventor 韩铮赵志辉董茂锋蒋可秋郭文博范楷聂冬霞
Owner SHANGHAI ACAD OF AGRI SCI
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