Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New method for simultaneous identification of toxoplasma gondii, rubella virus and herpes simplex virus

A herpes simplex virus and rubella virus technology, applied in the field of detection and identification, can solve the problems of wrong judgment of pathogens, unclear identification of pathogens, etc., and achieve the effect of accurate results, saving inspection costs, and saving reagents and sample requirements.

Active Publication Date: 2018-04-13
BINZHOU MEDICAL COLLEGE
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using classical methods, there is a possibility of errors that sometimes lead to unclear identification of the pathogen and thus to a wrong diagnosis of the pathogen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New method for simultaneous identification of toxoplasma gondii, rubella virus and herpes simplex virus
  • New method for simultaneous identification of toxoplasma gondii, rubella virus and herpes simplex virus
  • New method for simultaneous identification of toxoplasma gondii, rubella virus and herpes simplex virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A method for simultaneous detection and / or identification of Toxoplasma gondii, rubella virus and herpes simplex virus, including the following steps:

[0068] 1. Optimization of silicon wafer surface modification methods:

[0069] In this example, a variety of modification methods are used, and two modification methods are screened for optimization. The steps are as follows:

[0070] 1.1 Rinse the silicon wafer with deionized water 3 times, soak the silicon wafer in 1 mL of aminosilane and 3 mL of concentrated sulfuric acid for half an hour, rinse the silicon wafer 3 times in PBS, and soak the silicon wafer in 1 mL of glutaraldehyde and 3 mL of PBS for 1 hour to obtain an aldehyde-based chemical surface. Chip.

[0071] 1.2 Rinse the silicon wafer 3 times in deionized water, soak the silicon wafer in 1 mL of aminosilane and 3 mL of concentrated sulfuric acid for half an hour, rinse the silicon wafer 3 times in anhydrous alcohol, add saturated sodium succinate anhydrous alcohol ...

Embodiment 2

[0122] A kind of ellipsometric microscopic imaging protein chip, which is prepared by the following method:

[0123] Assemble the identification probes on the silicon wafers with carboxyl modified surfaces: assemble the antigens of Toxoplasma gondii, rubella virus and herpes simplex virus on the detection sites, and then wash them with PBS; then seal the detection sites with a blocking reagent to obtain Ellipsometry microscopic imaging protein chip; the preparation parameters refer to the parameters in the standardized detection and / or identification procedure in Example 1.

[0124] Quantitative detection of rubella virus antibody

[0125] 1. Calibration curve and quantitative detection of clinical serum samples

[0126] A calibration curve was established, and the serum samples (No. 942, 218 international units / ml) were serially diluted, and the dilution concentration was 0011, 0043, 0170, 0681, 2725 IU / mL rubella virus antibody with 5 concentration gradients, diluted with PBST. Rub...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

A disclosed novel method for identifying toxoplasma, rubella virus and herpes simplex virus at same time comprises (1) preparing an identification chip; (2) injecting a to-be-identified solution into detection sites of the identification chip in the step (1), after an antibody molecule in the to-be-identified solution is specifically combined with an antigen molecule on the identification chip and accordingly the thickness or density of the molecular film layer on the surface of the detection sites of the identification chip is changed, performing flushing; and (3) detecting the variation in the step (2) by using an ellipsometric microscopy imaging instrument, so as to determine whether the to-be-identified solution contain toxoplasma, rubella virus and herpes simplex virus and obtain the antibody content of toxoplasma, rubella virus and herpes simplex virus. The method is capable of rapidly accurately qualitatively and / or quantitatively determining toxoplasma, rubella virus and herpes simplex virus.

Description

Technical field [0001] The invention belongs to the technical field of detection and identification, and specifically relates to a new method for simultaneously identifying Toxoplasma gondii, rubella virus and herpes simplex virus. Background technique [0002] Toxoplasma gondii is an obligate intracellular parasite, coccidia, Eucoccidia, Isosporidae, and Toxoplasma. The worm is distributed worldwide and can be infected by humans and many animals. It can parasitize in all nucleated cells except red blood cells. It flows with the blood and reaches various parts of the body. It damages the brain, heart, and fundus of the eyes, resulting in a decline in the body’s immunity. Suffer from various diseases. The human body is mostly in a recessive infection state after infection. Only when the body's immunity is low, the worms are activated and multiply rapidly, and the pathogenicity is enhanced. As TOX poses a great threat to human health, the requirements for timeliness and accuracy ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569G01B11/06
CPCG01B11/0625G01N33/56905G01N33/56983G01N33/6803G01N2333/19G01N2333/45
Inventor 孙红柳王巧云
Owner BINZHOU MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com