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Glucolase biofuel cell

A glucose and enzyme electrode technology, applied in biochemical fuel cells, battery electrodes, circuits, etc., can solve the problems of unresolved reaction efficiency and inability to obtain solutions, and achieve large potential difference, strong ability to receive electrons, and increase contact area Effect

Inactive Publication Date: 2016-11-09
西安岳达生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No fundamental solution can be obtained without solving the problem of reaction efficiency

Method used

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  • Glucolase biofuel cell
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Embodiment

[0010] The enzyme purification method is: take 2.0mL of protease turbid solution in a test tube, add 2.0mL of saturated ammonium sulfate solution, stir evenly, let stand for 2 minutes, centrifuge at 4000r / min for 10 minutes, discard the supernatant, and precipitate the desired protein , add 2.0 mL of distilled water to dissolve the glucose dehydrogenase crude solution, and store at 4.0°C. The assay method of enzyme activity is: configure 10.0mM phosphate buffer (pH=7.4), 270g / L glucose solution, 60mM NADP (weigh 0.47244gNADP and dissolve to 10.0mL), take 2.7mL under normal temperature respectively Put phosphate buffer saline, 0.2mL glucose, and 0.1mL NADP in a cuvette, place it in a UV spectrophotometer, and return the absorbance to zero, take 0.05mL glucose dehydrogenase diluted 100 times with phosphate buffer solution and add it to the cuvette Mix well and start timing, read the absorbance value at the wavelength of 340nm at two minutes, the value read here is 0.522, which i...

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Abstract

The invention provides an enzyme electrode for redox reaction with glucose dehydrogenase serving as a catalyst. On the enzyme electrode, GDH (glucose dehydrogenase) and NADP (nicotinamide adenine dinucleotide phosphate) in an anode chamber catalyze glucose reaction to generate hydrogen ions and electrons, the hydrogen ions reach a cathode chamber through a proton exchange membrane between the two chambers, and the electrons reach a cathode electrode from an anode electrode through wire loading. Due to efficient performing of the redox reaction close to the electrodes, the enzyme electrode is capable of increasing obtained electric energy output, thereby being applicable to design of various fuel cells.

Description

technical field [0001] The invention relates to an enzyme biofuel cell. The enzymes that play a catalytic role carry out redox reactions and are modified to achieve higher output, which also involves the key component of the fuel cell, the enzyme electrode. Background technique [0002] Accompanying drawing has described the reaction scheme of general biofuel cell, and in the biofuel cell that uses glucose as fuel shown in the figure, the oxidation reaction of glucose is carried out at the negative electrode (as shown in figure (a)), and the oxygen in the atmosphere ( o 2 ) reduction reaction at the positive electrode (as shown in (b)). At the negative electrode, electrons are continuously transferred to glucose, glucose dehydrogenase, nicotinamide adenine dinucleotide (NAD + ), diaphorase, electron transfer mediator, and finally transferred to the carbon electrode. In contrast, at the positive electrode, electrons released from the negative electrode are continuously tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M8/16H01M4/86H01M4/90
CPCH01M4/86H01M4/90H01M8/16Y02E60/50
Inventor 张天卫泽明杨凡田萌
Owner 西安岳达生物科技股份有限公司